Mouse Anti-Arabidopsis AT4G33760 Antibody (CBMOAB-0140FYC)


Cat: CBMOAB-0140FYC
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityA. thaliana (Arabidopsis thaliana)
CloneMO0140FC
SpecificityThis antibody binds to Arabidopsis AT4G33760.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationChloroplast; Mitochondrion; Cytosol

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionCatalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
Product OverviewMouse Anti-Arabidopsis AT4G33760 (clone MO0140FC) Antibody (CBMOAB-0140FYC) is a mouse antibody against AT4G33760. It can be used for AT4G33760 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesPutative aspartate--tRNA ligase; At4g33760 At4g33760/T16L1_250
UniProt IDQ8GWM7
Protein RefseqThe length of the protein is 528 amino acids long. The sequence is show below: MRLEYVVLVEGTVRSRPNESVNKKMKTGFVEVVAEHVEILNPVRTKLPFLVTTADENKDLIKEEIRLRFRCLDLRRQRMKNNIVLRHNVVKLIRRYLEDRHGFIEIETPILSRSTPEGARDYLVPSRIQSGTFYALPQSPQLFKQMLMVSGFDKYYQIARCFRDEDLRADRQPEFTQLDMEMAFMPMEDMLKLNEDLIRKVFSEIKGIQLPDPFPRLTYADAMDRYGSDRPDTRFDLELKDVSNVFTESSFRVFTEALESGGIIKVLCVPLGAKKYSNSALKKGDIYNEAMKSGAKGLPFLKVLDNGEIEGIAALVSSLDSAGKINFVKQCGAAPGDLILFGVGPVTSVNKTLDRLRLFVAHDMDLIDHSKHSILWVTDFPMFEWNEPEQRLEALHHPFTAPKPEDMDDLPSARALAYDMVYNGVEIGGGSLRIYKRDVQEKVLEIIGISPEEAESKFGYLLEALDMGAPPHGGIAYGLDRMVMMLGGASSIRDVIAFPKTTTAQCALTRTPSEVDPKQLQDLSIRTK.

Reference

Reference1. Roland, M., Przybyla-Toscano, J., Vignols, F., Berger, N., Azam, T., Christ, L., ... & Rouhier, N. (2020). The plastidial Arabidopsis thaliana NFU1 protein binds and delivers [4Fe-4S] clusters to specific client proteins. Journal of Biological Chemistry, 295(6), 1727-1742.
2. Kitagawa, M., Balkunde, R., Bui, H., & Jackson, D. (2019). An aminoacyl tRNA synthetase, OKI1, is required for proper shoot meristem size in Arabidopsis. Plant and Cell Physiology, 60(11), 2597-2608.
3. Duchêne, A. M., Giritch, A., Hoffmann, B., Cognat, V., Lancelin, D., Peeters, N. M., ... & Small, I. D. (2005). Dual targeting is the rule for organellar aminoacyl-tRNA synthetases in Arabidopsis thaliana. Proceedings of the National Academy of Sciences, 102(45), 16484-16489.

Figure 1 In vitro import into organelles of targeting sequence-GFP fusions. In vitro transcriptions/translations products (lane 1) were incubated in the presence of mitochondria or of chloroplasts and were partially processed into smaller polypeptides (lane 2), which corresponded to the fusion proteins upon cleavage of the predicted targeting sequences.

For Research Use Only | Not For Clinical Use.

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