Mouse Anti-Arabidopsis NDHH Antibody (CBMOAB-37241FYC)


Cat: CBMOAB-37241FYC
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityA. thaliana (Arabidopsis thaliana)
CloneMO37241FC
SpecificityThis antibody binds to Arabidopsis NDHH.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationChloroplast

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionNDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.
Product OverviewMouse Anti-Arabidopsis NDHH Antibody is a mouse antibody against NDHH. It can be used for NDHH detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesNAD(P)H-quinone oxidoreductase subunit H, chloroplastic; EC 1.6.5.-; NAD(P)H dehydrogenase subunit H; NADH-plastoquinone oxidoreductase 49 kDa subunit; NADH-plastoquinone oxidoreductase subunit H; ndhH; AtCg01110
UniProt IDP56753
Protein RefseqThe length of the protein is 393 amino acids long. The sequence is show below: MKRPVTGKDLMIVNMGPHHPSMHGVLRLIVTLDGEDVVDCEPILGYLHRGMEKIAENRAIIQYLPYVTRWDYLATMFTEAITVNGPEQLGNIQVPKRASYIRVIMLELSRIASHLLWLGPFMADIGAQTPFFYIFREREFVYDLFEAATGMRMMHNFFRIGGIAADLPYGWIDKCLDFCDYFLTEVVEYQKLITRNPIFLERVEGVGIIGGEEAINWGLSGPMLRASGIPWDLRKIDRYESYDEFEWEIQWQKQGDSLARYLVRLSEMTESIKIIQQALEGLPGGPYENLESRGFDRKRNPEWNDFEYRFISKKPSPTFELSKQELYVRVEAPKGELGIFLIGDQSGFPWRWKIRPPGFINLQILPELVKRMKLADIMTILGSIDIIMGEVDR.

Reference

Reference1. Méteignier, L. V., Ghandour, R., Meierhoff, K., Zimmerman, A., Chicher, J., Baumberger, N., ... & Hammani, K. (2020). The Arabidopsis mTERF-repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons. New Phytologist, 227(5), 1376-1391.
2. Meurer, J., Berger, A., & Westhoff, P. (1996). A nuclear mutant of Arabidopsis with impaired stability on distinct transcripts of the plastid psbB, psbD/C, ndhH, and ndhC operons. The Plant Cell, 8(7), 1193-1207.
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CBMOAB-34962FYBMouse Anti-Rice ndhH Antibody (CBMOAB-34962FYB)
MO-AB-00719HMouse Anti-Arabidopsis ndhH Antibody (MO-AB-00719H)
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Figure 1 Run-on transcription assay of chloroplast genes in Arabidopsis mda1. Slot-blot analysis of run-on transcript samples from chloroplasts of the indicated genotypes hybridized to DNA fragments representing chloroplast rrn16, ndhH, ndhA, psaC and psbE genes.
Reference: Méteignier, L. V., Ghandour, R., Meierhoff, K., Zimmerman, A., Chicher, J., Baumberger, N., ... & Hammani, K. (2020). The Arabidopsis mTERF-repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons. New Phytologist, 227(5), 1376-1391.

For Research Use Only | Not For Clinical Use.
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