Rabbit Anti-ArfA Antibody (MO-DKB-02348W)


Cat: MO-DKB-02348W
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Specifications

Host speciesRabbit (Oryctolagus cuniculus)
Species ReactivityA. thaliana (Arabidopsis thaliana)
ImmunogenSynthetic peptides from Arabidopsis AT1G23490, AT5G14670, AT2G47170, AT1G70490, AT3G62290, AT301G, ArfAlA, ArfAlB, ArfAlC, ArfAlD, ArfAlE, ArfAlF.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
PurityPeptide affinity purified

Application Information

ApplicationWB
Application NotesWestern Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.

Target

IntroductionRescues ribosomes stalled at the 3' end of non-stop mRNAs (PubMed:21062370, PubMed:21435036). This activity is crucial when the stalled ribosome cannot be rescued by the SsrA(tmRNA)-SmpB quality control system (PubMed:21062370, PubMed:21435036). Binds the 30S subunit, contacting 16S rRNA with the N-terminus near the decoding center and its C-terminus in the mRNA entry channel; contacts change in the presence of release factor 2 (RF2, also named PrfB) (PubMed:25355516, PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875). Requires RF2/PrfB to hydrolyze stalled peptidyl-tRNA on the ribosome; recruits and probably helps position RF2/PrfB correctly in the ribosomal A site so RF2's GGQ motif can hydrolyze the peptidyl-tRNA bond (PubMed:22857598, PubMed:22922063, PubMed:25355516, PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875). Does not release ribosomes with a programmed pause caused by regulatory chains such as SecM-mediated pausing (PubMed:22857598). Binds tRNA which may stimulate its ribosome rescue activity (PubMed:22857598).
Product OverviewThis product is a Rabbit antibody against the ArfA. It can be used for ArfA detection in Western Blot.
Alternative NamesAlternative ribosome-rescue factor A; arfA; yhdL; b4550 JW3253; ArfA
See other products for " arfA "
For Research Use Only | Not For Clinical Use.
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