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Lot Number
- Product Details
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Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | C. elegans (Caenorhabditis elegans) |
| Clone | MO00176HB |
| Specificity | This antibody binds to C. elegans AAT9. |
| Format | Lyophilized |
| Storage | Store at 4°C: short-term (1-2 weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Product Overview | Mouse Anti-C. elegans AAT9 (clone MO00176HB) Antibody (CBMOAB-00176HCB) is a mouse antibody against AAT9. It can be used for AAT9 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | Protein AAT-9, isoform b; aat-9 |
| Gene ID | 190250 |
| UniProt ID | Q56VY0 |
| Protein Refseq | The length of the protein is 527 amino acids long. The sequence is show below: MSSIEDLPTVGDVAATRHQMGLWSCMSYVIANIIGAGIFITPGTVLKLALTNGMALVVWLGCGLISLIGGICYIELGTSIRDPGCDFAYNVYVGWEGIAFSFMWVGVIMSFPASAAVQAQTFGQYIVAGLSPIWHLGSPYDVILERGLGFALIIILTVLNLYAIDKYASKFQIFVTIAKLLSLAIIIVTGFWYLIVKGETEHFKDAFTPLPNEKYDIGQISLAFYGALWSFAGWDILNYGTPEIKNPRRTMPIALLGGISAVTLVYMAMNVSYMTVLDTETLKNSSAVAADFARITLGDFSYAIPFMISILLIGTLNSNIFCGSRFTHAAAREGHLPTFLSCINAESNSPRAALLFQLICTIAVTFVDTESLITYVTFVMFGQRVFTMAALLWIRYRNIPVHPDAIRVPLIFSILFFMITIALVVTPFIEDFTTTIVGVGLVLMGFLLYMIFMKPKQLPGFLYRFNDGITRVTCKILFTTPDLKHASEIETLKSDDESVVSNDERTTLSTSQIDTAAAGTAEPMTRL. |
See other products for " AAT9 "
| CBMOAB-00177HCB | Mouse Anti-C. elegans AAT9 Antibody (CBMOAB-00177HCB) |
IF
Figure 1 C. elegans AAT-9 transporter expressed alone in Xenopus oocytes is localized at the cell membrane. Immunofluorescence with anti-AAT-9 antibody on cryosections of Xenopus oocytes injected with AAT-9 cRNA (left) or H2O (control; right) shows AAT-9 at the cell surface.
IF
Figure 2 AAT-9 expression in the head region of wild type C. elegans. A, Nomarski image of the animal shown in panels B and C. B and C, the GFP fluorescence signal (green) is observed in neuronal bodies (arrows) and in body wall muscles (arrowheads) of aat-9::gfp transgenic animals. The upper arrow in panel B indicates a neuron possibly corresponding to the one labeled by immunofluorescence (panel D). The signal in the posterior region (panel B, asterisks) is considered autofluorescence. In a different focal plane of the same worm (panel C), a specific GFP signal is seen in a symmetrical pair of presumably sensory neurons (upper arrow) localized ventral to the pharynx. D, immunofluorescence with AAT-9 antibody (red) shows a signal at the level of a neuronal body (arrow). Nuclei are stained with 4,6-diamidino2-phenylindole (blue).