Rabbit Anti-crp Antibody (MO-DKB-00289W)


Cat: MO-DKB-00289W
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Specifications

Host speciesRabbit (Oryctolagus cuniculus)
Species ReactivityE. coli (Escherichia coli)
SpecificityThis antibody is binds to Escherichia coli crp.
ImmunogenRecombinant E. coli cAMP-activated global transcription regulator CRP protein
EpitopeThe epitope is in the AA 1-210 of crp.
FormatLiquid or Lyophilized
BufferPBS pH 7.4, 50% Glycerol
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 95% was determined by SDS-PAEG
PurificationPurified with G affinity chromatography

Application Information

ApplicationELISA

Target

IntroductionA global transcription regulator. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus sequence 5'-AAATGTGATCTAGATCACATTT-3') to directly regulate the transcription of about 300 genes in about 200 operons and indirectly regulate the expression of about half the genome. There are 3 classes of CRP promoters; class I promoters have a single CRP-binding site upstream of the RNA polymerase (RNAP)-binding site, whereas in class II promoters the single CRP- and RNAP-binding site overlap, CRP making multiple contacts with RNAP. Class III promoters require multiple activator molecules, including at least one CRP dimer. It can act as an activator, repressor, coactivator or corepressor. Induces a severe bend in DNA (about 87 degrees), bringing upstream promoter elements into contact with RNAP. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. High levels of active CRP are detrimental to growth (PubMed:16260780). Plays a major role in carbon catabolite repression (CCR). CCR involves cAMP, adenylate cyclase (cyaA), CRP and the EIIA-Glc component of the PTS (crr). In the presence of glucose EIIA-Glc is dephosphorylated, and does not activate adenylate cyclase, leading to reduced cAMP and thus decreased CRP activity. Also plays a role in many other processes (see PubMed:22573269).
Product OverviewThis product is a rabbit antibody against the AA 1-210 of the crp. It can be used for crp detection in Enzyme-Linked Immunosorbent Assay.
Alternative NamescAMP-activated global transcriptional regulator CRP; Catabolite activator protein; CAP; Catabolite gene activator; cAMP receptor protein; CRP; cAMP regulatory protein; crp; cap csm; b3357 JW5702
UniProt IDP0ACJ8
For Research Use Only | Not For Clinical Use.
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