Cat: MO-MMB-0549
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- Product Details
Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | C. elegans (Caenorhabditis elegans), Fruit fly (Drosophila melanogaster), Human (Homo sapiens), Rat (Rattus norvegicus), Yeast |
| Clone | M38F3 |
| Specificity | This clone was chosen because it is specific to ~34 kDa Fibrillarin. |
| Immunogen | Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae). |
| Format | Lyophilized |
| Regulatory Status | For Research Use Only |
| Shipping | Gel Pack |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Purity | Concentrated cell culture supernatant. |
Application Information
| Application | ICC, WB |
| Application Notes | WB: 1:1000-1:10000 ICC: 1:1000-1:5000 The optimal dilution should be determined by the end user. |
Target
| Introduction | Fibrillin is part of the small subunit processing complex and is involved in the processing of pre-18S ribosomal RNA. Nop1p is a yeast homolog of fibrillin. Fibrillarin/Nop1p is unusually conserved, so yeast and human proteins are 67% identical, and human proteins can functionally replace yeast proteins. This means that an appropriate cross-reactive antibody against Nop1p/fibrillarin (such as this antibody) can be used to reveal nucleoli and study fibrillarin/Nop1p in all eukaryotes and archaea tested so far. This antibody is being widely used as a convenient marker for nucleoli in many species. |
| Product Overview | This product is a mouse antibody against the FBRL. It can be used for FBRL detection in Immunocytochemistry, Western Blot. |
| Alternative Names | rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; FBL; FIB1; FLRN; Nop1p |
| UniProt ID | P15646 |
Reference
For Research Use Only | Not For Clinical Use.