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- Product Details
|Host species||Mouse (Mus musculus)|
|Species Reactivity||C. elegans (Caenorhabditis elegans), Fruit fly (Drosophila melanogaster), Human (Homo sapiens), Rat (Rattus norvegicus), Yeast|
|Specificity||This clone was chosen because it is specific to ~34 kDa Fibrillarin.|
|Immunogen||Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).|
|Regulatory Status||For Research Use Only|
|Storage||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
|Purity||Concentrated cell culture supernatant.|
|Application Notes||WB: 1:1000-1:10000|
The optimal dilution should be determined by the end user.
|Introduction||Fibrillin is part of the small subunit processing complex and is involved in the processing of pre-18S ribosomal RNA. Nop1p is a yeast homolog of fibrillin. Fibrillarin/Nop1p is unusually conserved, so yeast and human proteins are 67% identical, and human proteins can functionally replace yeast proteins. This means that an appropriate cross-reactive antibody against Nop1p/fibrillarin (such as this antibody) can be used to reveal nucleoli and study fibrillarin/Nop1p in all eukaryotes and archaea tested so far. This antibody is being widely used as a convenient marker for nucleoli in many species.|
|Product Overview||This product is a mouse antibody against the FBRL. It can be used for FBRL detection in Immunocytochemistry, Western Blot.|
|Alternative Names||rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; FBL; FIB1; FLRN; Nop1p|