Mouse Anti-FUT5 Antibody (CBMOAB-33595FYC)


Cat: CBMOAB-33595FYC
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  • Product List
  • Specifications
  • Application Information
  • Target
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  • Relate Reference Data
Sub Cat Clonality Species Reactivity Application Clone Conjugate Size  
CBMOAB-33595FYC Monoclonal A. thaliana (Arabidopsis thaliana), C. elegans (Caenorhabditis elegans), Cattle (Bos taurus), Chimpanzee (Pan troglodytes), Rhesus (Macaca mulatta) WB, ELISA MO33595FC 100 µg
CBMOAB-43229FYA Monoclonal Rhesus (Macaca mulatta) WB, ELISA MO43229FYA 100 µg
CBMOAB-04215HCB Monoclonal C. elegans (Caenorhabditis elegans) WB, ELISA MO04215HB 100 µg
MO-AB-15146W Monoclonal Chimpanzee (Pan troglodytes) WB, ELISA MO15146W 100 µg
MO-AB-12765R Monoclonal Cattle (Bos taurus) WB, ELISA MO12765R 100 µg

Specifications

Host speciesMouse (Mus musculus)
Species ReactivityA. thaliana (Arabidopsis thaliana), C. elegans (Caenorhabditis elegans), Cattle (Bos taurus), Chimpanzee (Pan troglodytes), Rhesus (Macaca mulatta)
CloneMO33595FC
SpecificityThis antibody binds to Arabidopsis FUT5.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationGolgi apparatus; Other locations

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionAtFUT3, AtFUT4 and AtFUT5 were expressed in tobacco (Nicotiana tabacum L. cv BY2) suspension culture cells and the resulting proteins did not transfer fucose (Fuc) from GDP-Fuc to tamarind xyloglucan. AtFUT3, AtFUT4, and AtFUT5 were overexpressed in Arabidopsis plants.
Product OverviewMouse Anti-Arabidopsis FUT5 (clone MO33595FC) Antibody (CBMOAB-33595FYC) is a mouse antibody against FUT5. It can be used for FUT5 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesFucosyltransferase 5; Galactoside 3-L-Fucosyltransferase; Alpha (1,3) Fucosyltransferase; Fucosyltransferase V; EC 2.4.1.65; FUC-TV; FucT-V; Alpha-(1,3)-Fucosyltransferase 5; EC 2.4.1
UniProt IDQ9SJP4
Protein RefseqThe length of the protein is 533 amino acids long. The sequence is show below: MYQKFQISGKIVKTLGLKMKVLIAVSFGSLLFILSYSNNFNNKLLDATTKVDIKETEKPVDKLIGGLLTADFDEGSCLSRYHKYFLYRKPSPYKPSEYLVSKLRSYEMLHKRCGPDTEYYKEAIEKLSRDDASESNGECRYIVWVAGYGLGNRLLTLASVFLYALLTERIILVDNRKDVSDLLCEPFPGTSWLLPLDFPMLNYTYAWGYNKEYPRCYGTMSEKHSINSTSIPPHLYMHNLHDSRDSDKLFVCQKDQSLIDKVPWLIVQANVYFVPSLWFNPTFQTELVKLFPQKETVFHHLARYLFHPTNEVWDMVTDYYHAHLSKADERLGIQIRVFGKPDGRFKHVIDQVISCTQREKLLPEFATPEESKVNISKTPKLKSVLVASLYPEFSGNLTNMFSKRPSSTGEIVEVYQPSGERVQQTDKKSHDQKALAEMYLLSLTDNIVTSARSTFGYVSYSLGGLKPWLLYQPTNFTTPNPPCVRSKSMEPCYLTPPSHGCEADWGTNSGKILPFVRHCEDLIYGGLKLYDEF.

Reference

Reference1. Sarria, R., Wagner, T. A., O'Neill, M. A., Faik, A., Wilkerson, C. G., Keegstra, K., & Raikhel, N. V. (2001). Characterization of a family of Arabidopsis genes related to xyloglucan fucosyltransferase1. Plant Physiology, 127(4), 1595-1606.
2. Ambrosone, A., Batelli, G., Nurcato, R., Aurilia, V., Punzo, P., Bangarusamy, D. K., ... & Grillo, S. (2015). The Arabidopsis RNA-binding protein AtRGGA regulates tolerance to salt and drought stress. Plant physiology, 168(1), 292-306.
3. Pogorelko, G. V., Reem, N. T., Young, Z. T., Chambers, L., & Zabotina, O. A. (2016). Post-synthetic defucosylation of agp by Aspergillus nidulans α-1, 2-fucosidase expressed in arabidopsis apoplast induces compensatory upregulation of α-1, 2-fucosyltransferases. PLoS One, 11(7), e0159757.

Relate Reference Data

Figure 1 RT-PCR showing the expression pattern of theAtFUT gene family. Southern analysis of RT-PCR products was done for genes that did not produce visible bands on ethidium bromide-stained gels.
Reference: Sarria, R., Wagner, T. A., O'Neill, M. A., Faik, A., Wilkerson, C. G., Keegstra, K., & Raikhel, N. V. (2001). Characterization of a family of Arabidopsis genes related to xyloglucan fucosyltransferase1. Plant Physiology, 127(4), 1595-1606.

For Research Use Only | Not For Clinical Use.
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