Anti-H3K27ac Antibody (Cat MO-MMB-0699), Rabbit IgG

Cat: MO-MMB-0699
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Host speciesRabbit (Oryctolagus cuniculus)
Species ReactivityBudding Yeast, Human (Homo sapiens)
ImmunogenThis Histone H3 acetyl Lys27 antibody is raised against peptides including the acetyl lysine 27 of histone H3.
FormatLiquid or Lyophilized
Buffer10 mM Sodium pH ospH ate, pH 7.5, 150 mM NaCl, 30% glycerol
Regulatory StatusFor Research Use Only
ShippingGel Pack
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
PurificationProtein A purified

Application Information

ApplicationChIP, ChIP-Seq, Native ChIP, WB, IF, IHC(P), PLA
Application NotesChIP: 10 µg per ChIP
ChIP-Seq: 5 µg each
IF: 1-5 µg/mL
WB: 0.1-1 µg/mL
The optimal dilution should be determined by the end user.


IntroductionHistone H3 is one of the core components of nucleosomes. The nucleosome is the smallest subunit of chromatin and consists of 147 DNA base pairs wrapped around the core histone octamer (histone H2A, histone H2B, histone H3, and histone H4). Histone H1 is a linking histone that exists at the interface between the nucleosome core and DNA entry/exit points; it is responsible for building higher-order chromatin structures. Chromatin undergoes a variety of chemical modifications, including post-translational modifications of histones and methylation of cytosine residues in DNA. The reported histone modifications include acetylation, methylation, phosphorylation, ubiquitination, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression.
Alternative NamesH3K27ac; Histone H3K27ac


For Research Use Only | Not For Clinical Use.

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