Anti-H3K4me3 Antibody (Cat MO-MMB-0697), Rabbit IgG


Cat: MO-MMB-0697
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Specifications

Host speciesRabbit (Oryctolagus cuniculus)
Species ReactivityBudding Yeast, Human (Homo sapiens)
ImmunogenThis histone H3 trimethyl Lys4 antibody is raised against peptides including the trimethyl lysine 4 of histone H3.
FormatLiquid or Lyophilized
BufferPBS, pH 7.5, 30% glycerol
Regulatory StatusFor Research Use Only
ShippingGel Pack
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
PurificationProtein A purified

Application Information

ApplicationChIP-qPCR, ChIP-Seq, Native ChIP, IF, WB
Application NotesChIP: 3 µg per ChIP
ChIP-Seq: 4 µg each
IF: 1-2 µg/mL
WB: 2-4 µg/mL
The optimal dilution should be determined by the end user.

Target

IntroductionHistone H3 is one of the core components of nucleosomes. The nucleosome is the smallest subunit of chromatin and consists of 147 DNA base pairs wrapped around the core histone octamer (histone H2A, histone H2B, histone H3, and histone H4). Histone H1 is a linking histone protein that exists at the interface between the nucleosome core and the DNA entry/exit point. Histone H1 is responsible for building higher-order chromatin structures. Chromatin undergoes a variety of chemical modifications, including post-translational modifications of histones and methylation of cytosine residues in DNA. The reported histone modifications include acetylation, methylation, phosphorylation, ubiquitination, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Alternative NamesH3K4me3; Histone H3K4me3
See other products for " H3K4me3 "
For Research Use Only | Not For Clinical Use.
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