Cat: MO-MMB-0698
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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- Product Details
Specifications
| Host species | Rabbit (Oryctolagus cuniculus) |
| Species Reactivity | Fission Yeast, Human (Homo sapiens) |
| Immunogen | This histone H3 trimethyl Lys9 antibody is raised against peptides including the trimethyl lysine 9 of histone H3. |
| Format | Liquid or Lyophilized |
| Buffer | 30% Glycerol |
| Regulatory Status | For Research Use Only |
| Shipping | Gel Pack |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
Application Information
| Application | ChIP, ChIP-Seq, Native ChIP, IF, WB |
| Application Notes | ChIP: 2-10 µL per ChIP ChIP-Seq: 10 µL each IF: 1:500-1:1000 WB: 1:1000-1:5000 The optimal dilution should be determined by the end user. |
Target
| Introduction | Histone H3 is one of the core components of nucleosomes. The nucleosome is the smallest subunit of chromatin and consists of 147 DNA base pairs wrapped around the core histone octamer (histone H2A, histone H2B, histone H3, and histone H4). Histone H1 is a linking histone protein that exists at the interface between the nucleosome core and the DNA entry/exit point. Histone H1 is responsible for building higher-order chromatin structures. Chromatin undergoes a variety of chemical modifications, including post-translational modifications of histones and methylation of cytosine residues in DNA. The reported histone modifications include acetylation, methylation, phosphorylation, ubiquitination, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. |
| Alternative Names | H3K9me3; Histone H3K9me3 |
Reference
For Research Use Only | Not For Clinical Use.