Rabbit Anti-lexA Antibody (MO-DKB-00177W)


Cat: MO-DKB-00177W
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Specifications

Host speciesRabbit (Oryctolagus cuniculus)
Species ReactivityE. coli (Escherichia coli)
SpecificityThis antibody is binds to Escherichia coli lexA.
ImmunogenRecombinant Escherichia coli LexA Repressor Protein
EpitopeThe epitope is in the AA 1-202 of lexA.
FormatLiquid or Lyophilized
BufferPBS pH 7.4, 50% Glycerol
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 95% was determined by SDS-PAEG
PurificationPurified with G affinity chromatography

Application Information

ApplicationELISA

Target

IntroductionRepresses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed:20005847).The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352).
Product OverviewThis product is a rabbit antibody against the AA 1-202 of the lexA. It can be used for lexA detection in Enzyme-Linked Immunosorbent Assay.
Alternative NamesLexA repressor; EC 3.4.21.88; lexA; exrA spr tsl umuA; b4043 JW4003
UniProt IDP0A7C2
For Research Use Only | Not For Clinical Use.
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