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Rabbit Anti-MAP1LC3B Antibody (AA 1-100)

Cat: MO-NAB-00358W
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Host speciesRabbit (Oryctolagus cuniculus)
Species ReactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus), Pig (Sus scrofa), Cattle (Bos taurus), Fish, Frog (Xenopus), Zebrafish (Danio rerio)
SpecificityThis antibody is binds to Human MAP1LC3B and has cross reactivity with Mouse, Rat, Pig, Cattle, Fish, Frog, Zebrafish.
ImmunogenRecombinant monoclonal LC3B antibody (1251A) is made of a synthetic peptide against the N-terminal part (between residues 1-100) of the human LC3B protein sequence.
EpitopeThe epitope is in the AA 1-100 of MAP1LC3B.
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PurificationProtein G purified
Cellular LocalizationType I form of LC3B is cytoplasmic; whereas the type II form of LC3B binds to the autophagic membranes

Application Information

ApplicationWB, FC, FC-IC, IF, IHC, IHC-Fr, IHC-P, IP, KO
Application NotesImmunofluorescence: 10-20 ug/ml
Immunohistochemistry: 1:100-1:500
Immunohistochemistry-Paraffin: 1:100-1:500
Immunoprecipitation: 2-10 ug


IntroductionMicrotubule-associated proteins 1A/1B light chain 3B (hereafter referred to as LC3) is a protein that in humans is encoded by the MAP1LC3B gene. LC3 is a central protein in the autophagy pathway where it functions in autophagy substrate selection and autophagosome biogenesis. LC3 is the most widely used marker of autophagosomes.
Product OverviewThis product is a Rabbit antibody against the AA 1-100 of the MAP1LC3B. It can be used for MAP1LC3B detection in Western Blot, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated.
Alternative NamesMAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta
Gene ID643246(Human)
UniProt IDA6NCE7(Human)
For Research Use Only | Not For Clinical Use.

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