Mouse Anti-PARP2 Antibody (CBMOAB-38138FYC)

Sub Cat	Clonality	Species Reactivity	Application	Clone	Conjugate	Size
CBMOAB-38138FYC	Monoclonal	A. thaliana (Arabidopsis thaliana), Chimpanzee (Pan troglodytes), Frog (Xenopus laevis), Rat (Rattus norvegicus), Rhesus (Macaca mulatta), Zebrafish (Danio rerio)	WB, ELISA	MO38138FC		100 µg	Inquiry
Protein Refseq: The length of the protein is 637 amino acids long. The sequence is show below: MANKLKVDELRLKLAERGLSTTGVKAVLVERLEEAIAEDTKKEESKSKRKRNSSNDTYESNKLIAIGEFRGMIVKELREEAIKRGLDTTGTKKDLLERLCNDANNVSNAPVKSSNGTDEAEDDNNGFEEEKKEEKIVTATKKGAAVLDQWIPDEIKSQYHVLQRGDDVYDAILNQTNVRDNNNKFFVLQVLESDSKKTYMVYTRWGRVGVKGQSKLDGPYDSWDRAIEIFTNKFNDKTKNYWSDRKEFIPHPKSYTWLEMDYGKEENDSPVNNDIPSSSSEVKPEQSKLDTRVAKFISLICNVSMMAQHMMEIGYNANKLPLGKISKSTISKGYEVLKRISEVIDRYDRTRLEELSGEFYTVIPHDFGFKKMSQFVIDTPQKLKQKIEMVEALGEIELATKLLSVDPGLQDDPLYYHYQQLNCGLTPVGNDSEEFSMVANYMENTHAKTHSGYTVEIAQLFRASRAVEADRFQQFSSSKNRMLLWHGSRLTNWAGILSQGLRIAPPEAPVTGYMFGKGVYFADMFSKSANYCYANTGANDGVLLLCEVALGDMNELLYSDYNADNLPPGKLSTKGVGKTAPNPSEAQTLEDGVVVPLGKPVERSCSKGMLLYNEYIVYNVEQIKMRYVIQVKFNYKH.
CBMOAB-53915FYA	Monoclonal	Rhesus (Macaca mulatta)	WB, ELISA	MO53915FYA		100 µg	Inquiry
Protein Refseq: The length of the protein is 534 amino acids long. The sequence is show below: MPVTGGNANEDRTEDKQDGTPGRSWASKRVSESVKALLLKGKVPVDPECTAKVGKAHVYCEGSNVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWMRWGRVGKMGQHSLVACSGNLNKAKEIFEKKFLDKTKNNWEDREKFEKVPGKYDMLQMDYATNTQDEEETKKEESLKSPLKPESQLDLRVQELIKLICNVQAMEEMMMEMKYNTKKAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRPLMEACNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTELQSPEHPLDQHYRNLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVEKEGEKEAFREDLHNRMLLWHGSRLSNWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASHLKNIGLLLLSEVALGQCNELLEANPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYIVYNPNQVRMRYLLKVQFNFLQLW.
CBMOAB-91460FYA	Monoclonal	Zebrafish (Danio rerio)	WB, ELISA	MO91460FYA		100 µg	Inquiry
Protein Refseq: The length of the protein is 649 amino acids long. The sequence is show below: MRRTRSCRDKSVADSGSEASEAKTVWQWKGDEDEWEPYPSEVCEQLDAAKQAGEKSVSLALGPGYVVDLVKMTQTNTVSKYKRKIRLQKVKSEDLNEGNALNGNKKATGVVQIKEEKEEEEEQPVSKKRRNEGRSQKANKDKPVVKEECEVVKTLVMKGKAPVDPECKAKLGKAHVYNEGADVYDVMLNQTNLQFNNNKYYLIQLLEDDSAKAYSVWLRWGRVGKVGQNNLVSCGANLAQAKDTFKKKFFDKTKNEWEHRASFEKVAGKYDMVFMDYSTEDKGEEKAVVPSATQKKPCQLNSKVQSLLELICDLKAMEECVLEMKFDTKKAPLGKLTAEQIRAGYASLKRIEECLKKKGSNKELLDACNQFYTRIPHDFGLRTPPIIRSEEELKEKITLLETLSDIQIAVKMVQSNVKSDEHPLDRQYHSLNCQLQPLDTDSNEYKVIEKYLKSTHAPTHTDYTMTLLDVFAVEREVEKDNFNSELQNRMLLWHGSRLSNWVGILSQGLRVAPAEAPVTGYMFGKGIYFADMSSKSANYCFASQKNNQGLLLLSEVALGDSNELLDADYNADQLPSGKHSTKGLGQTAPDPKKSVSLNGVTVPLGPSVKTGVGQKGGYSLLYNEYIVYNPAQIQMKYLLRVQFNFSSLW.
MO-AB-05002W	Monoclonal	Rhesus (Macaca mulatta)	WB, ELISA	MO05002W		100 µg	Inquiry
Protein Refseq: The length of the protein is 570 amino acids long. The sequence is show below: MAARRRRSTRGGRARALNESKRINNGNTAPEDSSPAKKTRRCQRQGSKKMPVTGGNANEDRTEDKQDESVKALLLKGKVPVDPECTAKVGKAHVYCEGSNVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWMRWGRVGKMGQHSLVACSGNLNKAKEIFEKKFLDKTKNNWEDREKFEKVPGKYDMLQMDYATNTQDEEETKKEESLKSPLKPESQLDLRVQELIKLICNVQAMEEMMMEMKYNTKKAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRPLMEACNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTELQSPEHPLDQHYRNLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVKKEGEKEAFREDLHNRMLLWHGSRLSNWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASHLKNIGLLLLSEVALGQCNELLEANPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYIVYNPNQVRMRYLLKVQFNFLQLW.
MO-AB-11020W	Monoclonal	Chimpanzee (Pan troglodytes)	WB, ELISA	MO11020W		100 µg	Inquiry
Protein Refseq: The length of the protein is 570 amino acids long. The sequence is show below: MAARRRRSTGGGRARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESKKMPVAGGKANKDRTEDKQDESVKALLLKGKAPVDPECTAKVGKAHVYCEGNDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWMRWGRVGKMGQHSLVACSGNLNKAKEIFQKKFLDKTKNNWEDREKFEKVPGKYDMLQMDYATNTQDEEETKKEESLKSPLKPESQLDLRVQELIKLICNVQAMEEMMMEMKYNTKKAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRALMEACNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTELQSPEHPLDQHYRNLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVEKDGEKEAFREDLHNRMLLWHGSRMSNWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEANPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYIVYNPNQVRMRYLLKVQFNFLQLW.
MO-AB-06023H	Monoclonal	Frog (Xenopus laevis)	WB, ELISA	MO06023C		100 µg	Inquiry
Protein Refseq: The length of the protein is 558 amino acids long. The sequence is show below: MVQKNSQTQYERQIQRSLSGSNSAPQQKSIISAPAKRARGSNSVRNVENESEENKEQVRTLIMKGKAPVDPECSIKLGKAHVFCEGDDVYDVMLNQTNLQFNNNKYYLIQLLEDDGVKNFSVWMRWGRVGKVGQHSLVSCGSDLQKSKDIFQKKFYEKTKNLWTERAQFEKCAGKYDMLQMDYNATEEEKETTVKEESMADVPKPESQLDASIQELIELICNLKAMEETVLEMKFDTKKAPLGKLTVEQIRAGYCSLQQIENCIKKQKFGRDLLEACNEFYTRIPHDFGLRTPPLIRTLEDLTVKVRLLEALGDIQIAVKLASLELSSLEHPFDRQYRQLNCSMQPLDQTSSTFQLIEGYLQSTHAPTHNDYTMTLLRVFELQRVGEECNFREDLPNRMLLWHGSRLTNWVGILSQGLRVAPPEAPVTGYMFGKGIYFADVSSKSANYCFSSRDKNVGVLLLSEVALGECNELLAADCDAQKKIKSKHSTKGLGRSIPDPKKSIIHEGAVVPLGPLMDTGLTNDGGYTLNYNEYIVYDPHQVRMKYLLQVRFNYSSLW.
MO-AB-27726H	Monoclonal	Rat (Rattus norvegicus)	WB, ELISA	MO27726C		100 µg	Inquiry
Protein Refseq: The length of the protein is 139 amino acids long. The sequence is show below: MAPRRRRSGCGRRVLNEAKRVDNGNRATDDSPPGKKVRTCQKKGPAAGGEDTDRTKGKQDSVKTLLVKGRAPVDPECTSKVGKAHVYCEGDDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQQNFSVWMRWGRGKGFMMD.
MO-DKB-0359RA	Polyclonal	A. thaliana (Arabidopsis thaliana)	WB			50 µL	Inquiry
MO-DKB-01691W	Polyclonal	A. thaliana (Arabidopsis thaliana)	WB			100 µg	Inquiry

Specifications

Host species	Mouse (Mus musculus)
Species Reactivity	A. thaliana (Arabidopsis thaliana), Chimpanzee (Pan troglodytes), Frog (Xenopus laevis), Rat (Rattus norvegicus), Rhesus (Macaca mulatta), Zebrafish (Danio rerio)
Clone	MO38138FC
Specificity	This antibody binds to Arabidopsis PARP2.
Format	Liquid or Lyophilized
Storage	Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use
Purity	> 90% was determined by SDS-PAGE
Purification	Purified with Protein A or G affinity chromatography
Cellular Localization	Nucleus

Application Information

Application	WB, ELISA
Application Notes	ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

Introduction	This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.
Product Overview	Mouse Anti-Arabidopsis PARP2 (clone MO38138FC) Antibody (CBMOAB-38138FYC) is a mouse antibody against PARP2. It can be used for PARP2 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative Names	Poly(ADP-Ribose) Polymerase 2; ADP-Ribosyltransferase (NAD+; Poly(ADP-Ribose) Polymerase)-Like 2; ADP-Ribosyltransferase Diphtheria Toxin-Like 2; Poly (ADP-Ribose) Polymerase Family, Member 2; NAD(+) ADP-Ribosyltransferase 2; Poly[ADP-Ribose] Synthase 2; EC 2.4.2.30; PADPRT-2; ADPRTL2; ADPRT-2
UniProt ID	Q11207
Protein Refseq	The length of the protein is 637 amino acids long. The sequence is show below: MANKLKVDELRLKLAERGLSTTGVKAVLVERLEEAIAEDTKKEESKSKRKRNSSNDTYESNKLIAIGEFRGMIVKELREEAIKRGLDTTGTKKDLLERLCNDANNVSNAPVKSSNGTDEAEDDNNGFEEEKKEEKIVTATKKGAAVLDQWIPDEIKSQYHVLQRGDDVYDAILNQTNVRDNNNKFFVLQVLESDSKKTYMVYTRWGRVGVKGQSKLDGPYDSWDRAIEIFTNKFNDKTKNYWSDRKEFIPHPKSYTWLEMDYGKEENDSPVNNDIPSSSSEVKPEQSKLDTRVAKFISLICNVSMMAQHMMEIGYNANKLPLGKISKSTISKGYEVLKRISEVIDRYDRTRLEELSGEFYTVIPHDFGFKKMSQFVIDTPQKLKQKIEMVEALGEIELATKLLSVDPGLQDDPLYYHYQQLNCGLTPVGNDSEEFSMVANYMENTHAKTHSGYTVEIAQLFRASRAVEADRFQQFSSSKNRMLLWHGSRLTNWAGILSQGLRIAPPEAPVTGYMFGKGVYFADMFSKSANYCYANTGANDGVLLLCEVALGDMNELLYSDYNADNLPPGKLSTKGVGKTAPNPSEAQTLEDGVVVPLGKPVERSCSKGMLLYNEYIVYNVEQIKMRYVIQVKFNYKH.

Relate Reference Data

WB

Figure 1 PARP2 plays a dominant role in DNA damage response after bleomycin treatment. Two-week old Arabidopsis plants were transferred to 0, 2.5, 5 or 10 μg/ml of bleomycin for 18 h. Total proteins were extracted, separated by SDS-PAGE and analyzed by immunoblotting using an anti-PAR antibody. Equivalent loading of lanes was verified using Ponceau S stain. All samples shown and both blots were processed in parallel within the same experiment. Similar results were obtained in three separate experiments.

WB

Figure 2 PARP2 plays a dominant role in response to ionizing irradiation. Two-week old Arabidopsis plants grown on MS plates were irradiated with 150 Gy of γ-radiation and then flash-frozen 20, 40 or 60 min after removal from the radiation source. Total proteins were then extracted, separated by SDS-PAGE and analyzed by immunoblotting with an anti-PAR antibody. 0 min sample not exposed to γ-radiation source. All samples shown in (A) were processed in parallel within the same experiment.