AibGenesis™ Mouse Anti-SGF29 Antibody (CBMOAB-03926CR)


Cat: CBMOAB-03926CR

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Lot Number

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  • Specifications
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Sub Cat Clonality Species Reactivity Application Clone Conjugate Size  
CBMOAB-03926CR Monoclonal Yeast, Chimpanzee (Pan troglodytes), Fruit fly (Drosophila melanogaster) WB, ELISA MO03926CR 100 µg
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CBMOAB-30897FYA Monoclonal Fruit fly (Drosophila melanogaster) WB, ELISA MO30897FYA 100 µg
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MO-AB-12744W Monoclonal Chimpanzee (Pan troglodytes) WB, ELISA MO12744W 100 µg
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityYeast, Chimpanzee (Pan troglodytes), Fruit fly (Drosophila melanogaster)
CloneMO03926CR
SpecificityThis antibody binds to Yeast SGF29.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationNucleus

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionChromatin reader component of the transcription regulatory histone acetylation (HAT) complexes SAGA and SLIK (PubMed:10026213, PubMed:15647753, PubMed:21685874, PubMed:24307402). In the SAGA complex, SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PubMed:21685874). SGF29 is also required for heterochromatin boundary formation function (PubMed:24307402). SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes At the promoters, SAGA is required for recruitment of the basal transcription machinery (PubMed:10026213). It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8) (PubMed:10026213). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs) (PubMed:10026213). SLIK is proposed to have partly overlapping functions with SAGA (PubMed:15647753). It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus (PubMed:15647753). (From uniprot, under CC BY 4.0)
Product OverviewMouse Anti-Yeast SGF29 Antibody is a mouse antibody against SGF29. It can be used for SGF29 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesSAGA-associated factor 29; 29 kDa SAGA-associated factor; SAGA histone acetyltransferase complex 29 kDa subunit; SGF29; YCL010C
UniProt IDP25554
Protein RefseqThe length of the protein is 259 amino acids long. The sequence is show below: MDGYWDVVVSSLQDIYNANEVIPFDDELQTKKLNFLNMSKDQLQLHLNTFQEHMENVNRVHRILDNVRSNLSLMLNQSREEKSEENTEDAEEGEGTRMALSQGKKAVGKVGRSYWTSEYNPNAPILVGSEVAYKPRRGSADGEWIQCEVLKVVADGTRFEVRDPEPDELGNSGKVYKCNRKELLLIPPGFPTKNYPPGTKVLARYPETTTFYPAIVIGTKRDGTCRLRFDGEEEVDKETEVTRRLVLPSPTALANLARK.
For Research Use Only | Not For Clinical Use.
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