Cat: CBMOAB-0149YC
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- Product Details
Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | E. coli (Escherichia coli ) |
| Clone | MO0149YC |
| Specificity | This antibody binds to Escherichia coli K-12 argP. |
| Format | Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Introduction | Controls the transcription of genes involved in arginine and lysine metabolism. Activates transcription of several genes, including argO, lysP, lysC, asd, dapB, dapD, lysA, gdhA and argK. Acts by binding directly to their promoter or control region (PubMed:10600368, PubMed:15150242, PubMed:17504942, PubMed:21441513, PubMed:21890697). ArgP dimer by itself is able to bind the argO promoter-operator region to form a binary complex, but the formation of a ternary complex with RNA polymerase is greatly stimulated only in presence of a coeffector. Both arginine and lysine are coeffectors at the argO promoter, but only arginine is competent to activate transcription. Lysine has repressive effects (PubMed:15150242, PubMed:17504942). ArgP also mediates lysine repression of dapB, and gdhA in vivo, but via an alternative mechanism: ArgP binding is directly reduced upon the addition of lysine (PubMed:21890697). Binds in vitro to the promoter region of dnaA and to the upstream region of the nrd promoter, but these genes are probably not regulated by ArgP in vivo (PubMed:9254708, PubMed:9819053, PubMed:21890697). In vitro, binds also to the three 13-mers located in the origin region (oriC) and blocks the initiation of replication (PubMed:1733927). |
| Product Overview | Mouse Anti-E. coli argP (clone MO0149YC) Antibody (CBMOAB-0149YC) is a mouse antibody against argP. It can be used for argP detection in Western Blot and Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | HTH-type transcriptional regulator ArgP; Inhibitor of chromosome initiation; OriC replication inhibitor; argP; iciA; b2916 JW2883 |
| UniProt ID | P0A8S1 |
| Protein Refseq | The length of the protein is 297 amino acids long. The sequence is show below: MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENMFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSLATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLGALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPCHIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPESRMMRKVTDALLDYGHKVLRQD. |
For Research Use Only | Not For Clinical Use.