NovaBlue(DE3) competent cells (MOFY-0822-FY508)


Cat: MOFY-0822-FY508
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Specifications

DescriptionNovaBlue (DE3) is derived from the K12 strain and has the highest transformation efficiency. It is a prokaryotic expression strain with high transformation efficiency. It can be used for the construction of common plasmids and the prokaryotic expression of proteins at the same time. The λ phage DE3 region is integrated into the chromosomal DNA of NovaBlue(DE3) strain, so that NovaBlue(DE3) strain can express T7 RNA polymerase and E. coli RNA polymerase at the same time, which is widely used for protein expression of pET series, pGEX, pMAL and other plasmids.
Gene TypesF`[ proA+B+ lacIq ZΔM15::Tn10 (TetR)] endA1 hsdR17 (rk12-,mk12+) supE44 thi -1 recA1 gyrA96 relA1 lac (DE3)
Size100 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection>5×109 cfu/μg DNA
Note1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency.
2. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating.
3. In order to obtain the required amount of protein, the induction time, temperature, and IPTG concentration need to be optimized by the experimenter.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
For Research Use Only | Not For Clinical Use.
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