pBT3-STE membrane protein two-hybrid plasmid

Cat: MOFY-0123-FY14
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  • Product Details


Species SourceYeast
Size2 µg
CompositionPromoter: CYC1 promoter;
Replicon: CEN/ARS (1-2 copies/cell);
Terminator: CYC1 terminator;
Plasmid size: 7614bp;
Prokaryotic resistance: Kanamycin Kan;
Cloned strain: Escherichia coli DH5α;
Culture conditions: 37°C, aerobic, LB.
BufferRefer to COA

Plasmid Information

Product OverviewpBT3-STE is a membrane protein yeast two-hybrid plasmid. The DUALmembrane system is a yeast-based screening assay to identify and characterize protein-protein interactions between integral membrane proteins, membrane-associated proteins and soluble proteins in their natural setting. When using the DUALmembrane system interactions between full-length integral membrane proteins can be detected in situ at cellular membranes.
Regulatory StatusFor Research Use Only
ShippingDry ice
StorageStore at -20 °C.
References1. Kittanakom*, S., Chuk*, M., Wong, V., Snyder, J., Edmonds, D., Lydakis, A., ... & Stagljar, I. (2009). Analysis of membrane protein complexes using the split-ubiquitin membrane yeast two-hybrid system. Yeast Functional Genomics and Proteomics: Methods and Protocols, 247-271.
2. Lentze, N., & Auerbach, D. (2008). Membrane-based yeast two-hybrid system to detect protein interactions. Current Protocols in Protein Science, 52(1), 19-17.
For Research Use Only | Not For Clinical Use.
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