Tuner(DE3) competent cells (MOFY-0822-FY507)
Cat: MOFY-0822-FY507
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Specifications
| Description | The Tuner (DE3) strain is a mutant of the lacZY gene (galactosidyl permease gene) of the BL21 strain. This mutation causes IPTG to enter each cell of E. coli in the system at a uniform rate, resulting in a more stringent and uniform concentration dependence. |
| Gene Types | F- ompT hsdSB (rB- mB- ) gal dcm lacY1 (DE3) |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 1×107 cfu/μg DNA |
| Note | 1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Be gentle when mixing plasmids. 3. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating. 4. During induction, IPTG concentration is optional (0.1-2mM can be used). 5. In order to obtain the required amount of protein, the induction time, temperature, and IPTG concentration need to be optimized by the experimenter. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
Related Products
| MOFY-0822-FY506 | Tuner(DE3)pLysS competent cells (MOFY-0822-FY506) |
For Research Use Only | Not For Clinical Use.
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