Mouse Anti-Arabidopsis TPP2 Antibody (CBMOAB-45225FYC)
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Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | A. thaliana (Arabidopsis thaliana) |
| Clone | MO45225FC |
| Specificity | This antibody binds to Arabidopsis TPP2. |
| Format | Liquid or Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
| Cellular Localization | Other locations; Chloroplast; Vacuole; Cytosol |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Introduction | This gene encodes a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class. |
| Product Overview | Mouse Anti-Arabidopsis TPP2 (clone MO45225FC) Antibody (CBMOAB-45225FYC) is a mouse antibody against TPP2. It can be used for TPP2 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | Tripeptidyl Peptidase 2; Tripeptidyl Aminopeptidase; Tripeptidyl-Peptidase II; EC 3.4.14.10; TPP-II; TPP-2; Tripeptidyl Peptidase II; Tripeptidyl-Peptidase 2; TPPII |
| UniProt ID | F4JVN6 |
| Protein Refseq | The length of the protein is 1380 amino acids long. The sequence is show below: MDLSLQLQIHGALINKGPSCTSYWASSSSLSLPRDFISSSTFLLHRRLRRRSCSRSRGIRLRRSGFSAMPCSSSDTLTASRVGCGGGGGGGAVGGGAENASVANFKLNESTFIASLMPKKEIRADCFIEAHPEYDGRGVVIAIFDSGFDPSAAGLHVTSDGKPKVLDVIDCTGSGDIDTSTVVKANEDGHIRGASGATLVVNSSWKNPTGEWRVGSKLVYQLFTDDLTSRVKKERRKSWDEKNQEEIAKAVNNLYDFDQKHSKVEDAKLKKTREDLQSKVDFLKKQADKYEDKGPVIDAVVWHDGEVWRVALDTQSLEEDPDSGKLADFSPLTNYRIERKYGVFSRLDACSFVANVYDEGKVLSIVTDSSPHGTHVAGIATAHHPEEHLLNGVAPGAQIISCKIGDSRLGSMETGTGLTRALIAALEHNCDLVNMSYGEPALLPDYGRFVDLVTEAVNKRRLIFVSSAGNSGPALTTVGAPGGTTSSIIGVGAYVSPAMAAGAHSVVEPPSEGLEYTWSSRGPTSDGDLGVCISAPGGAVAPVPTWTLQRRMLMNGTSMASPSACGAIALLLSAMKAEGIPVSPYSVRRALENTSTPVGDLPEDKLTTGQGLMQVDKAYEYLKQFQDYPCVFYQIKVNLSGKTIPTSRGIYLREGTACRQSTEWTIQVDPKFHEGASNLKELVPFEECLELHSTDEGVVRVPDYLLLTNNGRGFNVVVDPTNLGDGVHYFEVYGIDCKAPERGPLFRIPVTIIIPKTVANQPPVISFQQMSFISGHIERRYIEVPHGATWAEATMRTSGFDTTRRFYIDTLQVCPLRRPIKWESAPTFASPSAKSFVFPVVSGQTMELAIAQFWSSGLGSREPTIVDFEIEFHGVGVDKEELLLDGSEAPIKVEAEALLASEKLVPIAVLNKIRVPYQPIDAQLKTLSTGRDRLLSGKQILALTLTYKFKLEDSAEVKPYIPLLNNRIYDTKFESQFFMISDTNKRVYAMGDVYPESSKLPKGEYKLQLYLRHENVELLEKLKQLTVFIERNMGEIRLNLHSEPDGPFTGNGAFKSSVLMPGVKEAFYLGPPTKDKLPKNTPQGSMLVGEISYGKLSFDEKEGKNPKDNPVSYPISYVVPPNKPEEDKKAASAPTCSKSVSERLEQEVRDTKIKFLGNLKQETEEERSEWRKLCTCLKSEYPDYTPLLAKILEGLLSRSDAGDKISHHEEIIEAANEVVRSVDVDELARFLLDKTEPEDDEAEKLKKKMEVTRDQLADALYQKGLAMARIENLKGEKEGEGEEESSQKDKFEENFKELTKWVDVKSSKYGTLTVLREKRLSRLGTALKVLDDLIQNENETANKKLYELKLDLLEEIGWSHLVTYEKQWMQVRFPKSLPLF. |
| MO-AB-08664H | Mouse Anti-Frog tpp2 Antibody (MO-AB-08664H) |
| MO-AB-13933W | Mouse Anti-Chimpanzee TPP2 Antibody (MO-AB-13933W) |
| CBMOAB-89700FYB | Mouse Anti-Rice TPP2 Antibody (CBMOAB-89700FYB) |
| MO-AB-46898W | Mouse Anti-Horse TPP2 Antibody (MO-AB-46898W) |
| CBMOAB-12277HCB | Mouse Anti-C. elegans TPP2 Antibody (CBMOAB-12277HCB) |
| MO-AB-66773W | Mouse Anti-Marmoset TPP2 Antibody (MO-AB-66773W) |
| CBMOAB-60938FYA | Mouse Anti-Rhesus TPP2 Antibody (CBMOAB-60938FYA) |
| MO-AB-22081R | Mouse Anti-Cattle TPP2 Antibody (MO-AB-22081R) |
SDS-PAGE
Figure 1 Purification of TPPII from Arabidopsis seedlings. SDS-PAGE analysis of the TPPII purification steps. Samples from the crude extract, 2% to 8% PEG precipitation, pooled fractions from the UnoQ anion-exchange column, and the peak fraction from the Superose HR6 column were subjected to SDS-PAGE and silver staining. Positions of the 153- and 142-kD TPPII species are indicated.
SDS-PAGE
Figure 2 Native PAGE of Arabidopsis TPPII. The peak fraction from the Superose HR6 FPLC was subjected to native PAGE and TPPII was either detected by fluorescence following overlay of the gel with the substrate AAF-AMC or by staining with silver nitrate. Migration positions of the 26S proteasome and its 20S CP in an adjacent lane are indicated. For two-dimensional PAGE, TPPII was first subjected to native PAGE followed by SDS-PAGE in the second dimension and the gel was then stained with silver nitrate. The arrow indicates the electrophoretic direction of the SDS-PAGE. The positions of the 153- and 142-kD forms of TPPII are shown. Arrowheads locate the series of discrete size species of TPPII observed by native PAGE.