Glycobiology

Background

Glycobiology

Glycobiology, also known as glycan, is the study of the structure, biosynthesis, biology, and evolution of saccharides that are widely distributed in nature in all living forms. Glycobiology is a rapidly expanding field of study that is critical for both cellular biology and studies of carbohydrate-based drugs and therapeutics:

  • Glycans play a role in communication between cells and their external environment.
  • Glycosylation is important for protein folding and quality control and defines the adhesive properties of proteins and cells.
  • Glycans play an important role in the development of multicellular organisms and their functions are determined by their unique structures.
  • Glycosylation is an important determinant of the efficacy of biologics such as therapeutic antibodies.

Research Area of Glycobiology in Different Species

  • Yeasts

As biopharmaceuticals with humanized N-linked oligosaccharides, the suppression of yeast-specific O-mannosylation is important to reduce immune response and to improve heterologous protein productivity in the production of biopharmaceuticals. Present study demonstrated that Saccharomyces cerevisiae strain is capable of producing a glycoprotein with humanized Man5GlcNAc2 N-linked oligosaccharides, an intermediate of mammalian hybrid- and complex-type oligosaccharides while suppressing O-mannosylation. This strain was generated by firstly introducing msdS encoding α-1,2-mannosidase into a strain synthesizing Man8GlcNAc2 N-linked oligosaccharides, then disrupting PMT1 and PMT2, using a mutagenesis technique that is based on the disparity theory of evolution and finally disrupting vacuolar proteases PEP4 and PRB1.

  • Rice

Although the differences in protein glycosylation between sexes have already been observed in the rice pest insect Nilaparvata lugens (N. lugens), the functionality of differential N-glycosylation between sexes is yet unknown. Recent study indicated that comparison of N-glycopeptides sites from the adult stages of N. lugens revealed striking differences in protein N-glycosylation between sexes. Additionally, differential glycan composition between males and females was observed for proteins shared across sexes.

  • Arabidopsis thaliana (Arabidopsis)

N-glycosylation plays multiple roles in regulating stress tolerance of plants. Study has shown that an obvious decrease in photosynthetic capacity and dry mass were detected in alg3-3 and cgl1-1, two typical mutants in N-glycosylation process of Arabidopsis. The maximal photochemical efficiency of PSII, which reflected the photochemical of plant, decreased significantly in cgl1-1. Also, a similar tendency was observed in alg3-3. Besides, N-glycosylation was also required to maintain the stability of a chloroplast-located protein CAH1, which was closely related to photosynthesis.

Creative Biolabs offers a comprehensive range of monoclonal, polyclonal, and secondary antibodies. All our antibodies provide high levels of specificity and affinity to target antigens. We also provide a range of cell line controls for reliable antibody validation. For further details, please feel free to contact us.

Reference

  1. Lakshminarayanan, Abirami.; et al. Studying glycobiology at the single-molecule level. Nature Reviews Chemistry. 2018.

Our provided featured target antibody products including but not limited to:

Cat# Product Name Host species Species Reactivity Application Size Conjugate Alternative Names Protein
CBMOAB-00431HCB Mouse Anti-C. elegans AGL1 Antibody (CBMOAB-00431HCB) Mouse C. elegans (Caenorhabditis elegans) WB, ELISA 0.5mg, 1mg, 200µg Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme, agl-1 4-alpha-glucanotransferase
CBMOAB-04249HCB Mouse Anti-C. elegans GALT1 Antibody (CBMOAB-04249HCB) Mouse C. elegans (Caenorhabditis elegans) WB, ELISA 0.5mg, 1mg, 200µg Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP Protein GALT-1, galt-1 Beta-1,4-galactosyltransferase galt-1
MORAB-011F Anti-ALG6 Antibody (Cat MORAB-011F), Human IgG Human Saccharomyces cerevisiae IA 0.5mg, 1mg, 10mg None ALG6, YOR002W, UNA544 Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
MOFAB-008W Rabbit Anti-PPP1R3G Antibody (MOFAB-008W) Rabbit Zebrafish ELISA, WB, IP Inquiry None PPP1R3G, PPP1R3G PPP1R3G
MOFAB-030W mouse Anti-Rubisco Antibody (MOFAB-030W) mouse Rice IA Inquiry None Ribulose-1, 5-bisphosphate carboxylase/oxygenase, Rubisco Ribulose-1, 5-bisphosphate carboxylase/oxygenase
MOFAB-184W Rabbit Anti-UGPase Antibody (MOFAB-184W) Rabbit Arabidopsis, Canola, Brassica Oleracea WB, IF Inquiry None Probable UTP--glucose-1-phosphate uridylyltransferase 2, EC 2.7.7.9, UDP-glucose pyrophosphorylase 2, UDPGP 2, UGPase 2, At3g03250, UGPase, UGPase Probable UTP--glucose-1-phosphate uridylyltransferase 2
MOF032922W82 Rabbit Anti-Gliadin Antibody (MOF032922W82) Rabbit Wheat gliadin ELISA, IHC-P, IHC-Fr, ICC, IF 100µL, 200µL, 50µL NONE Celiac disease, gliadin - wheat ergot protein
MOFY-0522-FY12 Mouse Anti-CD1.1 Antibody (MOFY-0522-FY12) Mouse Chicken FC, IHC, IP 0.5mg Biotin, FITC, NONE CD1.1 CD1.1 antigen
MOFY-0522-FY20 Mouse Anti-B6.1 Antibody (MOFY-0522-FY20) Mouse Chicken FC, IHC, IP 0.5mg Biotin, FITC, NONE, PE B6.2, Bu-1, Bu-1b LOC396098
MOFY-0522-FY22 Mouse Anti-B6.1 Antibody (MOFY-0522-FY22) Mouse Chicken FC 100µg Alexa Fluor®488, Alexa Fluor®647, Biotin, FITC, NONE, PACBLU, PE B6.2, Bu-1, Bu-1b LOC396098
MOFY-0522-FY23 Mouse Anti-CD44 Antibody (MOFY-0522-FY23) Mouse Chicken, Turkey, Pigeon FC, IHC 0.5mg APC, Biotin, FITC, NONE, PE, SPRD CD44 Molecule (Indian Blood Group), Hematopoietic Cell E- And L-Selectin Ligand, GP90 Lymphocyte Homing/Adhesion Receptor, Chondroitin Sulfate Proteoglycan 8, Extracellular Matrix Receptor III, Heparan Sulfate Proteoglycan, Phagocytic Glycoprotein 1, Hyaluronate Receptor, Hermes Antigen, CD44 Antigen, ECMR-III, HUTCH-I, Epican, CDW44, MDU2, MDU3 CD44 Molecule (Indian Blood Group)
MOFY-0522-FY29 Mouse Anti-B6.1 Antibody (MOFY-0522-FY29) Mouse Chicken FC, IHC, IP, Apop 0.5mg Biotin, FITC, NONE, PE Bu-1a, B6.2, Bu-1, Bu-1b LOC396098
MOFY-0522-FY122 Rat Anti-Laminin Antibody (MOFY-0522-FY122) Rat Human, Mouse, Swine IHC, IF, IHC-Fr 100µg NONE Laminin Laminin
MOFY-0522-FY203 Mouse Anti-BLV gp51-G Antibody (MOFY-0522-FY203) Mouse Bovine RIPA, FC, ELISA 100µg NONE BLV gp51-G, Bovine Leukemia Virus (BLV) MAb gp51-G Bovine Leukemia Virus (BLV) MAb gp51-G
MOFY-0522-FY204 Mouse Anti-BLV gp51-G D-D Antibody (MOFY-0522-FY204) Mouse Bovine RIPA, FC, WB 100µg NONE BLV gp51-G D-D', Bovine Leukemia Virus (BLV) MAb gp51 D-D' Bovine Leukemia Virus (BLV) MAb gp51 D-D'
MOFY-0522-FY50 Rat Anti-ICAM1 Antibody (MOFY-0522-FY50) Rat Mouse, Porcine FC, IHC, WB, IP 0.5mg Alexa Fluor®700, APC, Biotin, FITC, LE/AF, NONE, PACBLU, PE Intercellular Adhesion Molecule 1 Intercellular Adhesion Molecule 1
MOFY-0522-FY60 Mouse Anti-SIRPA Antibody (MOFY-0522-FY60) Mouse Porcine FC 100µg Alexa Fluor®488, Biotin, FITC, NONE, PE Signal Regulatory Protein Alpha, CD172 Antigen-Like Family Member A, Inhibitory Receptor SHPS-1, Macrophage Fusion Receptor, PTPNS1, SHPS1, SIRP, P84, BIT, MFR, Brain-Immunoglobulin-Like Molecule With Tyrosine-Based Activation Motifs, Brain Ig-Like Molecule With Tyrosine-Based Activation Motifs, Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1, Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1, Tyrosine Phosphatase SHP Substrate 1, Signal-Regulatory Protein Alpha-1 Signal Regulatory Protein Alpha
MOFY-0522-FY70 Mouse Anti-CD8 Antibody (MOFY-0522-FY70) Mouse Feline, Lion, Tsushima FC, IHC, IP 0.5mg Biotin, FITC, NONE, PE CD8 alpha chain, CD8 CD8 alpha chain
MOFY-0522-FY82 Mouse Anti-FBN1 Antibody (MOFY-0522-FY82) Mouse Human, Bovine, Japanese IHC, EM, WB, IP 200µg Biotin, FITC, NONE Fibrillin 1, Asprosin, FBN, Fibrillin 1 (Marfan Syndrome), Fibrillin-1 Preproprotein, Marfan Syndrome, Fibrillin 15, Fibrillin-1, GPHYSD2, ACMICD Fibrillin 1
MOFY-0722-FY262 Rabbit Anti-FSHb Antibody (MOFY-0722-FY262) Rabbit Pig, Mouse WB, IHC, ICC, IP 100µL None Follicle Stimulating Hormone Beta Subunit, Follicle Stimulating Hormone, Beta Polypeptide, Follitropin, Beta Chain, FSH-Beta, FSH-B, Follicle-Stimulating Hormone Beta Subunit, Follitropin Subunit Beta, Follitropin Beta Chain, HH24 Follicle Stimulating Hormone Beta Subunit
MOFY-0722-FY268 Rabbit Anti-FGB Antibody (MOFY-0722-FY268) Rabbit Pig WB, IHC, ICC, IP 100µL None Fibrinogen Beta Chain, Fibrinogen, B Beta Polypeptide, Epididymis Secretory Sperm Binding Protein Li 78p, Beta-Fibrinogen, HEL-S-78p Fibrinogen Beta Chain
MOFY-0722-FY317 Rabbit Anti-TG Antibody (MOFY-0722-FY317) Rabbit Bovine WB, IHC, ICC, IP 100µL None Thyroglobulin, AITD3, TGN, Tg TG Gene(Protein Coding) Thyroglobulin
MOFY-0722-FY423 Rabbit Anti-FN1 Antibody (MOFY-0722-FY423) Rabbit Goat WB, IHC, ICC, IP 100µL None Fibronectin 1, Cold-Insoluble Globulin, Migration-Stimulating Factor, CIG, FN, Fibronectin, GFND2, SMDCF Fibronectin 1
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