Glycobiology

Background

Glycobiology

Glycobiology, also known as glycan, is the study of the structure, biosynthesis, biology, and evolution of saccharides that are widely distributed in nature in all living forms. Glycobiology is a rapidly expanding field of study that is critical for both cellular biology and studies of carbohydrate-based drugs and therapeutics:

  • Glycans play a role in communication between cells and their external environment.
  • Glycosylation is important for protein folding and quality control and defines the adhesive properties of proteins and cells.
  • Glycans play an important role in the development of multicellular organisms and their functions are determined by their unique structures.
  • Glycosylation is an important determinant of the efficacy of biologics such as therapeutic antibodies.

Research Area of Glycobiology in Different Species

  • Yeasts

As biopharmaceuticals with humanized N-linked oligosaccharides, the suppression of yeast-specific O-mannosylation is important to reduce immune response and to improve heterologous protein productivity in the production of biopharmaceuticals. Present study demonstrated that Saccharomyces cerevisiae strain is capable of producing a glycoprotein with humanized Man5GlcNAc2 N-linked oligosaccharides, an intermediate of mammalian hybrid- and complex-type oligosaccharides while suppressing O-mannosylation. This strain was generated by firstly introducing msdS encoding α-1,2-mannosidase into a strain synthesizing Man8GlcNAc2 N-linked oligosaccharides, then disrupting PMT1 and PMT2, using a mutagenesis technique that is based on the disparity theory of evolution and finally disrupting vacuolar proteases PEP4 and PRB1.

  • Rice

Although the differences in protein glycosylation between sexes have already been observed in the rice pest insect Nilaparvata lugens (N. lugens), the functionality of differential N-glycosylation between sexes is yet unknown. Recent study indicated that comparison of N-glycopeptides sites from the adult stages of N. lugens revealed striking differences in protein N-glycosylation between sexes. Additionally, differential glycan composition between males and females was observed for proteins shared across sexes.

  • Arabidopsis thaliana (Arabidopsis)

N-glycosylation plays multiple roles in regulating stress tolerance of plants. Study has shown that an obvious decrease in photosynthetic capacity and dry mass were detected in alg3-3 and cgl1-1, two typical mutants in N-glycosylation process of Arabidopsis. The maximal photochemical efficiency of PSII, which reflected the photochemical of plant, decreased significantly in cgl1-1. Also, a similar tendency was observed in alg3-3. Besides, N-glycosylation was also required to maintain the stability of a chloroplast-located protein CAH1, which was closely related to photosynthesis.

Creative Biolabs offers a comprehensive range of monoclonal, polyclonal, and secondary antibodies. All our antibodies provide high levels of specificity and affinity to target antigens. We also provide a range of cell line controls for reliable antibody validation. For further details, please feel free to contact us.

Reference

  1. Lakshminarayanan, Abirami.; et al. Studying glycobiology at the single-molecule level. Nature Reviews Chemistry. 2018.

Our provided featured target antibody products including but not limited to:

  • Mouse Anti-C. elegans AGL1 Antibody (CBMOAB-00431HCB) (CAT#: CBMOAB-00431HCB)

    • Host species: Mouse
    • Species Reactivity: C. elegans (Caenorhabditis elegans)
    • Application: WB, ELISA
    • Protein: 4-alpha-glucanotransferase
    • Size: 0.5mg, 1mg, 200µg
    • Conjugate: Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP
    • Alternative Names: AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme, agl-1

  • Mouse Anti-C. elegans GALT1 Antibody (CBMOAB-04249HCB) (CAT#: CBMOAB-04249HCB)

    • Host species: Mouse
    • Species Reactivity: C. elegans (Caenorhabditis elegans)
    • Application: WB, ELISA
    • Protein: Beta-1,4-galactosyltransferase galt-1
    • Size: 0.5mg, 1mg, 200µg
    • Conjugate: Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP
    • Alternative Names: Protein GALT-1, galt-1

  • Anti-ALG6 Antibody (Cat MORAB-011F), Human IgG (CAT#: MORAB-011F)

    • Host species: Human
    • Species Reactivity: Saccharomyces cerevisiae
    • Application: IA
    • Protein: Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
    • Size: 0.5mg, 1mg, 10mg
    • Conjugate: None
    • Alternative Names: ALG6, YOR002W, UNA544

  • Rabbit Anti-PPP1R3G Antibody (MOFAB-008W) (CAT#: MOFAB-008W)

    • Host species: Rabbit
    • Species Reactivity: Zebrafish
    • Application: ELISA, WB, IP
    • Protein: PPP1R3G
    • Size: NONE
    • Conjugate: None
    • Alternative Names: PPP1R3G, PPP1R3G

  • mouse Anti-Rubisco Antibody (MOFAB-030W) (CAT#: MOFAB-030W)

    • Host species: mouse
    • Species Reactivity: Rice
    • Application: IA
    • Protein: Ribulose-1, 5-bisphosphate carboxylase/oxygenase
    • Size: NONE
    • Conjugate: None
    • Alternative Names: Ribulose-1, 5-bisphosphate carboxylase/oxygenase, Rubisco

  • Rabbit Anti-UGPase Antibody (MOFAB-184W) (CAT#: MOFAB-184W)

    • Host species: Rabbit
    • Species Reactivity: Arabidopsis, Canola, Brassica Oleracea
    • Application: WB, IF
    • Protein: Probable UTP--glucose-1-phosphate uridylyltransferase 2
    • Size: NONE
    • Conjugate: None
    • Alternative Names: Probable UTP--glucose-1-phosphate uridylyltransferase 2, EC 2.7.7.9, UDP-glucose pyrophosphorylase 2, UDPGP 2, UGPase 2, At3g03250, UGPase, UGPase

  • Rabbit Anti-Gliadin Antibody (MOF032922W82) (CAT#: MOF032922W82)

    • Host species: Rabbit
    • Species Reactivity: Wheat gliadin
    • Application: ELISA, IHC-P, IHC-Fr, ICC, IF
    • Protein: ergot protein
    • Size:
    • Conjugate:
    • Alternative Names: Celiac disease, gliadin - wheat
    • COA

  • Mouse Anti-CD1.1 Antibody (MOFY-0522-FY12) (CAT#: MOFY-0522-FY12)

    • Host species: Mouse
    • Species Reactivity: Chicken
    • Application: FC, IHC, IP
    • Protein: CD1.1 antigen
    • Size: 0.5mg
    • Conjugate: Biotin, FITC, NONE
    • Alternative Names: CD1.1

  • Mouse Anti-B6.1 Antibody (MOFY-0522-FY20) (CAT#: MOFY-0522-FY20)

    • Host species: Mouse
    • Species Reactivity: Chicken
    • Application: FC, IHC, IP
    • Protein: LOC396098
    • Size: 0.5mg
    • Conjugate: Biotin, FITC, NONE, PE
    • Alternative Names: B6.2, Bu-1, Bu-1b

  • Mouse Anti-B6.1 Antibody (MOFY-0522-FY22) (CAT#: MOFY-0522-FY22)

    • Host species: Mouse
    • Species Reactivity: Chicken
    • Application: FC
    • Protein: LOC396098
    • Size: 100µg
    • Conjugate: Alexa Fluor®488, Alexa Fluor®647, Biotin, FITC, NONE, PACBLU, PE
    • Alternative Names: B6.2, Bu-1, Bu-1b

  • Mouse Anti-CD44 Antibody (MOFY-0522-FY23) (CAT#: MOFY-0522-FY23)

    • Host species: Mouse
    • Species Reactivity: Chicken, Turkey, Pigeon
    • Application: FC, IHC
    • Protein: CD44 Molecule (Indian Blood Group)
    • Size: 0.5mg
    • Conjugate: APC, Biotin, FITC, NONE, PE, SPRD
    • Alternative Names: CD44 Molecule (Indian Blood Group), Hematopoietic Cell E- And L-Selectin Ligand, GP90 Lymphocyte Homing/Adhesion Receptor, Chondroitin Sulfate Proteoglycan 8, Extracellular Matrix Receptor III, Heparan Sulfate Proteoglycan, Phagocytic Glycoprotein 1, Hyaluronate Receptor, Hermes Antigen, CD44 Antigen, ECMR-III, HUTCH-I, Epican, CDW44, MDU2, MDU3

  • Mouse Anti-B6.1 Antibody (MOFY-0522-FY29) (CAT#: MOFY-0522-FY29)

    • Host species: Mouse
    • Species Reactivity: Chicken
    • Application: FC, IHC, IP, Apop
    • Protein: LOC396098
    • Size: 0.5mg
    • Conjugate: Biotin, FITC, NONE, PE
    • Alternative Names: Bu-1a, B6.2, Bu-1, Bu-1b

  • Rat Anti-Laminin Antibody (MOFY-0522-FY122) (CAT#: MOFY-0522-FY122)

    • Host species: Rat
    • Species Reactivity: Human, Mouse, Swine
    • Application: IHC, IF, IHC-Fr
    • Protein: Laminin
    • Size: 100µg
    • Conjugate: NONE
    • Alternative Names: Laminin

  • Mouse Anti-BLV gp51-G Antibody (MOFY-0522-FY203) (CAT#: MOFY-0522-FY203)

    • Host species: Mouse
    • Species Reactivity: Bovine
    • Application: RIPA, FC, ELISA
    • Protein: Bovine Leukemia Virus (BLV) MAb gp51-G
    • Size:
    • Conjugate:
    • Alternative Names: BLV gp51-G, Bovine Leukemia Virus (BLV) MAb gp51-G
    • COA

  • Mouse Anti-BLV gp51-G D-D Antibody (MOFY-0522-FY204) (CAT#: MOFY-0522-FY204)

    • Host species: Mouse
    • Species Reactivity: Bovine
    • Application: RIPA, FC, WB
    • Protein: Bovine Leukemia Virus (BLV) MAb gp51 D-D'
    • Size:
    • Conjugate:
    • Alternative Names: BLV gp51-G D-D', Bovine Leukemia Virus (BLV) MAb gp51 D-D'
    • COA

  • Rat Anti-ICAM1 Antibody (MOFY-0522-FY50) (CAT#: MOFY-0522-FY50)

    • Host species: Rat
    • Species Reactivity: Mouse, Porcine
    • Application: FC, IHC, WB, IP
    • Protein: Intercellular Adhesion Molecule 1
    • Size: 0.5mg
    • Conjugate: Alexa Fluor®700, APC, Biotin, FITC, LE/AF, NONE, PACBLU, PE
    • Alternative Names: Intercellular Adhesion Molecule 1

  • Mouse Anti-SIRPA Antibody (MOFY-0522-FY60) (CAT#: MOFY-0522-FY60)

    • Host species: Mouse
    • Species Reactivity: Porcine
    • Application: FC
    • Protein: Signal Regulatory Protein Alpha
    • Size: 100µg
    • Conjugate: Alexa Fluor®488, Biotin, FITC, NONE, PE
    • Alternative Names: Signal Regulatory Protein Alpha, CD172 Antigen-Like Family Member A, Inhibitory Receptor SHPS-1, Macrophage Fusion Receptor, PTPNS1, SHPS1, SIRP, P84, BIT, MFR, Brain-Immunoglobulin-Like Molecule With Tyrosine-Based Activation Motifs, Brain Ig-Like Molecule With Tyrosine-Based Activation Motifs, Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1, Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1, Tyrosine Phosphatase SHP Substrate 1, Signal-Regulatory Protein Alpha-1

  • Mouse Anti-CD8 Antibody (MOFY-0522-FY70) (CAT#: MOFY-0522-FY70)

    • Host species: Mouse
    • Species Reactivity: Feline, Lion, Tsushima
    • Application: FC, IHC, IP
    • Protein: CD8 alpha chain
    • Size: 0.5mg
    • Conjugate: Biotin, FITC, NONE, PE
    • Alternative Names: CD8 alpha chain, CD8

  • Mouse Anti-FBN1 Antibody (MOFY-0522-FY82) (CAT#: MOFY-0522-FY82)

    • Host species: Mouse
    • Species Reactivity: Human, Bovine, Japanese
    • Application: IHC, EM, WB, IP
    • Protein: Fibrillin 1
    • Size: 200µg
    • Conjugate: Biotin, FITC, NONE
    • Alternative Names: Fibrillin 1, Asprosin, FBN, Fibrillin 1 (Marfan Syndrome), Fibrillin-1 Preproprotein, Marfan Syndrome, Fibrillin 15, Fibrillin-1, GPHYSD2, ACMICD

  • Rabbit Anti-FSHb Antibody (MOFY-0722-FY262) (CAT#: MOFY-0722-FY262)

    • Host species: Rabbit
    • Species Reactivity: Pig, Mouse
    • Application: WB, IHC, ICC, IP
    • Protein: Follicle Stimulating Hormone Beta Subunit
    • Size: 100µL
    • Conjugate: None
    • Alternative Names: Follicle Stimulating Hormone Beta Subunit, Follicle Stimulating Hormone, Beta Polypeptide, Follitropin, Beta Chain, FSH-Beta, FSH-B, Follicle-Stimulating Hormone Beta Subunit, Follitropin Subunit Beta, Follitropin Beta Chain, HH24

  • Rabbit Anti-FGB Antibody (MOFY-0722-FY268) (CAT#: MOFY-0722-FY268)

    • Host species: Rabbit
    • Species Reactivity: Pig
    • Application: WB, IHC, ICC, IP
    • Protein: Fibrinogen Beta Chain
    • Size: 100µL
    • Conjugate: None
    • Alternative Names: Fibrinogen Beta Chain, Fibrinogen, B Beta Polypeptide, Epididymis Secretory Sperm Binding Protein Li 78p, Beta-Fibrinogen, HEL-S-78p

  • Rabbit Anti-TG Antibody (MOFY-0722-FY317) (CAT#: MOFY-0722-FY317)

    • Host species: Rabbit
    • Species Reactivity: Bovine
    • Application: WB, IHC, ICC, IP
    • Protein: TG Gene(Protein Coding) Thyroglobulin
    • Size: 100µL
    • Conjugate: None
    • Alternative Names: Thyroglobulin, AITD3, TGN, Tg

  • Rabbit Anti-FN1 Antibody (MOFY-0722-FY423) (CAT#: MOFY-0722-FY423)

    • Host species: Rabbit
    • Species Reactivity: Goat
    • Application: WB, IHC, ICC, IP
    • Protein: Fibronectin 1
    • Size: 100µL
    • Conjugate: None
    • Alternative Names: Fibronectin 1, Cold-Insoluble Globulin, Migration-Stimulating Factor, CIG, FN, Fibronectin, GFND2, SMDCF

  • Mouse Anti-Endoglycosidase [EndoS] (AA 1-243) Antibody, IgG1 kappa (MOFY-0922-FY396) (CAT#: MOFY-0922-FY396)

    • Product Overview: MOFY-0922-FY396 is a mouse antibody against Endoglycosidase [EndoS]. It can be used for Endoglycosidase [EndoS] detection in Western Blot, Immunoprecipitation, Immunofluorescence, ELISA.
    • Host species: Mouse
    • Species Reactivity: S. equi subsp. equi 4047
    • Application: WB, IP, IF, ELISA
    • Protein: Endoglycosidase [EndoS]
    • Size:
    • Alternative Names: Endoglycosidase [EndoS]
    • COA
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