Background

Glycobiology, also known as glycan, is the study of the structure, biosynthesis, biology, and evolution of saccharides that are widely distributed in nature in all living forms. Glycobiology is a rapidly expanding field of study that is critical for both cellular biology and studies of carbohydrate-based drugs and therapeutics:
- Glycans play a role in communication between cells and their external environment.
- Glycosylation is important for protein folding and quality control and defines the adhesive properties of proteins and cells.
- Glycans play an important role in the development of multicellular organisms and their functions are determined by their unique structures.
- Glycosylation is an important determinant of the efficacy of biologics such as therapeutic antibodies.
Research Area of Glycobiology in Different Species
- Yeasts
As biopharmaceuticals with humanized N-linked oligosaccharides, the suppression of yeast-specific O-mannosylation is important to reduce immune response and to improve heterologous protein productivity in the production of biopharmaceuticals. Present study demonstrated that Saccharomyces cerevisiae strain is capable of producing a glycoprotein with humanized Man5GlcNAc2 N-linked oligosaccharides, an intermediate of mammalian hybrid- and complex-type oligosaccharides while suppressing O-mannosylation. This strain was generated by firstly introducing msdS encoding α-1,2-mannosidase into a strain synthesizing Man8GlcNAc2 N-linked oligosaccharides, then disrupting PMT1 and PMT2, using a mutagenesis technique that is based on the disparity theory of evolution and finally disrupting vacuolar proteases PEP4 and PRB1.
- Rice
Although the differences in protein glycosylation between sexes have already been observed in the rice pest insect Nilaparvata lugens (N. lugens), the functionality of differential N-glycosylation between sexes is yet unknown. Recent study indicated that comparison of N-glycopeptides sites from the adult stages of N. lugens revealed striking differences in protein N-glycosylation between sexes. Additionally, differential glycan composition between males and females was observed for proteins shared across sexes.
- Arabidopsis thaliana (Arabidopsis)
N-glycosylation plays multiple roles in regulating stress tolerance of plants. Study has shown that an obvious decrease in photosynthetic capacity and dry mass were detected in alg3-3 and cgl1-1, two typical mutants in N-glycosylation process of Arabidopsis. The maximal photochemical efficiency of PSII, which reflected the photochemical of plant, decreased significantly in cgl1-1. Also, a similar tendency was observed in alg3-3. Besides, N-glycosylation was also required to maintain the stability of a chloroplast-located protein CAH1, which was closely related to photosynthesis.
Reference
- Lakshminarayanan, Abirami.; et al. Studying glycobiology at the single-molecule level. Nature Reviews Chemistry. 2018.
Our provided featured target antibody products including but not limited to:
Cat# | Product Name | Host species | Species Reactivity | Application | Size | Conjugate | Alternative Names | Protein |
CBMOAB-00431HCB | Mouse Anti-C. elegans AGL1 Antibody (CBMOAB-00431HCB) | Mouse | C. elegans (Caenorhabditis elegans) | WB, ELISA | 0.5mg, 1mg, 200µg | Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP | AGL (Amylo-1,6-GLucosidase, 4-alpha-glucanotransferase) glycogen debranching enzyme, agl-1 | 4-alpha-glucanotransferase |
CBMOAB-04249HCB | Mouse Anti-C. elegans GALT1 Antibody (CBMOAB-04249HCB) | Mouse | C. elegans (Caenorhabditis elegans) | WB, ELISA | 0.5mg, 1mg, 200µg | Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP | Protein GALT-1, galt-1 | Beta-1,4-galactosyltransferase galt-1 |
MORAB-011F | Anti-ALG6 Antibody (Cat MORAB-011F), Human IgG | Human | Saccharomyces cerevisiae | IA | 0.5mg, 1mg, 10mg | None | ALG6, YOR002W, UNA544 | Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase |
MOFAB-008W | Rabbit Anti-PPP1R3G Antibody (MOFAB-008W) | Rabbit | Zebrafish | ELISA, WB, IP | Inquiry | None | PPP1R3G, PPP1R3G | PPP1R3G |
MOFAB-030W | mouse Anti-Rubisco Antibody (MOFAB-030W) | mouse | Rice | IA | Inquiry | None | Ribulose-1, 5-bisphosphate carboxylase/oxygenase, Rubisco | Ribulose-1, 5-bisphosphate carboxylase/oxygenase |
MOFAB-184W | Rabbit Anti-UGPase Antibody (MOFAB-184W) | Rabbit | Arabidopsis, Canola, Brassica Oleracea | WB, IF | Inquiry | None | Probable UTP--glucose-1-phosphate uridylyltransferase 2, EC 2.7.7.9, UDP-glucose pyrophosphorylase 2, UDPGP 2, UGPase 2, At3g03250, UGPase, UGPase | Probable UTP--glucose-1-phosphate uridylyltransferase 2 |
MOF032922W82 | Rabbit Anti-Gliadin Antibody (MOF032922W82) | Rabbit | Wheat gliadin | ELISA, IHC-P, IHC-Fr, ICC, IF | 100µL, 200µL, 50µL | NONE | Celiac disease, gliadin - wheat | ergot protein |
MOFY-0522-FY12 | Mouse Anti-CD1.1 Antibody (MOFY-0522-FY12) | Mouse | Chicken | FC, IHC, IP | 0.5mg | Biotin, FITC, NONE | CD1.1 | CD1.1 antigen |
MOFY-0522-FY20 | Mouse Anti-B6.1 Antibody (MOFY-0522-FY20) | Mouse | Chicken | FC, IHC, IP | 0.5mg | Biotin, FITC, NONE, PE | B6.2, Bu-1, Bu-1b | LOC396098 |
MOFY-0522-FY22 | Mouse Anti-B6.1 Antibody (MOFY-0522-FY22) | Mouse | Chicken | FC | 100µg | Alexa Fluor®488, Alexa Fluor®647, Biotin, FITC, NONE, PACBLU, PE | B6.2, Bu-1, Bu-1b | LOC396098 |
MOFY-0522-FY23 | Mouse Anti-CD44 Antibody (MOFY-0522-FY23) | Mouse | Chicken, Turkey, Pigeon | FC, IHC | 0.5mg | APC, Biotin, FITC, NONE, PE, SPRD | CD44 Molecule (Indian Blood Group), Hematopoietic Cell E- And L-Selectin Ligand, GP90 Lymphocyte Homing/Adhesion Receptor, Chondroitin Sulfate Proteoglycan 8, Extracellular Matrix Receptor III, Heparan Sulfate Proteoglycan, Phagocytic Glycoprotein 1, Hyaluronate Receptor, Hermes Antigen, CD44 Antigen, ECMR-III, HUTCH-I, Epican, CDW44, MDU2, MDU3 | CD44 Molecule (Indian Blood Group) |
MOFY-0522-FY29 | Mouse Anti-B6.1 Antibody (MOFY-0522-FY29) | Mouse | Chicken | FC, IHC, IP, Apop | 0.5mg | Biotin, FITC, NONE, PE | Bu-1a, B6.2, Bu-1, Bu-1b | LOC396098 |
MOFY-0522-FY122 | Rat Anti-Laminin Antibody (MOFY-0522-FY122) | Rat | Human, Mouse, Swine | IHC, IF, IHC-Fr | 100µg | NONE | Laminin | Laminin |
MOFY-0522-FY203 | Mouse Anti-BLV gp51-G Antibody (MOFY-0522-FY203) | Mouse | Bovine | RIPA, FC, ELISA | 100µg | NONE | BLV gp51-G, Bovine Leukemia Virus (BLV) MAb gp51-G | Bovine Leukemia Virus (BLV) MAb gp51-G |
MOFY-0522-FY204 | Mouse Anti-BLV gp51-G D-D Antibody (MOFY-0522-FY204) | Mouse | Bovine | RIPA, FC, WB | 100µg | NONE | BLV gp51-G D-D', Bovine Leukemia Virus (BLV) MAb gp51 D-D' | Bovine Leukemia Virus (BLV) MAb gp51 D-D' |
MOFY-0522-FY50 | Rat Anti-ICAM1 Antibody (MOFY-0522-FY50) | Rat | Mouse, Porcine | FC, IHC, WB, IP | 0.5mg | Alexa Fluor®700, APC, Biotin, FITC, LE/AF, NONE, PACBLU, PE | Intercellular Adhesion Molecule 1 | Intercellular Adhesion Molecule 1 |
MOFY-0522-FY60 | Mouse Anti-SIRPA Antibody (MOFY-0522-FY60) | Mouse | Porcine | FC | 100µg | Alexa Fluor®488, Biotin, FITC, NONE, PE | Signal Regulatory Protein Alpha, CD172 Antigen-Like Family Member A, Inhibitory Receptor SHPS-1, Macrophage Fusion Receptor, PTPNS1, SHPS1, SIRP, P84, BIT, MFR, Brain-Immunoglobulin-Like Molecule With Tyrosine-Based Activation Motifs, Brain Ig-Like Molecule With Tyrosine-Based Activation Motifs, Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1, Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1, Tyrosine Phosphatase SHP Substrate 1, Signal-Regulatory Protein Alpha-1 | Signal Regulatory Protein Alpha |
MOFY-0522-FY70 | Mouse Anti-CD8 Antibody (MOFY-0522-FY70) | Mouse | Feline, Lion, Tsushima | FC, IHC, IP | 0.5mg | Biotin, FITC, NONE, PE | CD8 alpha chain, CD8 | CD8 alpha chain |
MOFY-0522-FY82 | Mouse Anti-FBN1 Antibody (MOFY-0522-FY82) | Mouse | Human, Bovine, Japanese | IHC, EM, WB, IP | 200µg | Biotin, FITC, NONE | Fibrillin 1, Asprosin, FBN, Fibrillin 1 (Marfan Syndrome), Fibrillin-1 Preproprotein, Marfan Syndrome, Fibrillin 15, Fibrillin-1, GPHYSD2, ACMICD | Fibrillin 1 |
MOFY-0722-FY262 | Rabbit Anti-FSHb Antibody (MOFY-0722-FY262) | Rabbit | Pig, Mouse | WB, IHC, ICC, IP | 100µL | None | Follicle Stimulating Hormone Beta Subunit, Follicle Stimulating Hormone, Beta Polypeptide, Follitropin, Beta Chain, FSH-Beta, FSH-B, Follicle-Stimulating Hormone Beta Subunit, Follitropin Subunit Beta, Follitropin Beta Chain, HH24 | Follicle Stimulating Hormone Beta Subunit |
MOFY-0722-FY268 | Rabbit Anti-FGB Antibody (MOFY-0722-FY268) | Rabbit | Pig | WB, IHC, ICC, IP | 100µL | None | Fibrinogen Beta Chain, Fibrinogen, B Beta Polypeptide, Epididymis Secretory Sperm Binding Protein Li 78p, Beta-Fibrinogen, HEL-S-78p | Fibrinogen Beta Chain |
MOFY-0722-FY317 | Rabbit Anti-TG Antibody (MOFY-0722-FY317) | Rabbit | Bovine | WB, IHC, ICC, IP | 100µL | None | Thyroglobulin, AITD3, TGN, Tg | TG Gene(Protein Coding) Thyroglobulin |
MOFY-0722-FY423 | Rabbit Anti-FN1 Antibody (MOFY-0722-FY423) | Rabbit | Goat | WB, IHC, ICC, IP | 100µL | None | Fibronectin 1, Cold-Insoluble Globulin, Migration-Stimulating Factor, CIG, FN, Fibronectin, GFND2, SMDCF | Fibronectin 1 |
MOFY-0922-FY396 | Mouse Anti-Endoglycosidase [EndoS] (AA 1-243) Antibody, IgG1 kappa (MOFY-0922-FY396) | Mouse | S. equi subsp. equi 4047 | WB, IP, IF, ELISA | 200µg | Endoglycosidase [EndoS] | Endoglycosidase [EndoS] | |
MOFY-0922-FY405 | Mouse Anti-OVA Antibody, IgG1 kappa (MOFY-0922-FY405) | Mouse | Chicken | WB, IP | 200µg | OVA, Ovalbumin | Ovalbumin | |
MOFY-0922-FY406 | Mouse Anti-OVA Antibody, IgG1 (MOFY-0922-FY406) | Mouse | Chicken | IP, ELISA | 200µg | OVA, Ovalbumin | Ovalbumin | |
MOFY-0922-FY407 | Mouse Anti-OVA Antibody, IgG1 (MOFY-0922-FY407) | Mouse | Chicken | WB, ELISA | 200µg | OVA, Ovalbumin | Ovalbumin | |
MOFY-0922-FY408 | Mouse Anti-OVA Antibody, IgG2b (MOFY-0922-FY408) | Mouse | Chicken | WB, ELISA | 200µg | OVA, Ovalbumin | Ovalbumin | |
MOFY-0922-FY409 | Mouse Anti-OVA Antibody, IgG1 (MOFY-0922-FY409) | Mouse | Chicken | WB, ELISA | 200µg | OVA, Ovalbumin | Ovalbumin | |
MOFY-0922-FY410 | Mouse Anti-OVA Antibody, IgG1 (MOFY-0922-FY410) | Mouse | Chicken | IP, ELISA | 200µg | OVA, Ovalbumin | Ovalbumin |