Mouse Anti-Arabidopsis ATG10 Antibody (CBMOAB-24499FYC)


Cat: CBMOAB-24499FYC
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityA. thaliana (Arabidopsis thaliana)
CloneMO24499FC
SpecificityThis antibody binds to Arabidopsis ATG10.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionAutophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003).
Product OverviewMouse Anti-Arabidopsis ATG10 Antibody is a mouse antibody against ATG10. It can be used for ATG10 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesAutophagy Related 10; Autophagy-Related Protein 10; APG10L; ATG10 Autophagy Related 10 Homolog (S. Cerevisiae); APG10 Autophagy 10-Like (S. Cerevisiae); Ubiquitin-Like-Conjugating Enzyme ATG10; ATG10 Autophagy Related 10 Homolog
UniProt IDQ8VZ52
Protein RefseqThe length of the protein is 225 amino acids long. The sequence is show below: MDSAREVSDGRLTVEGFSVASRAFADKWKIHNQSFPPWSWVPLINRTLLVSKKEEGYLSLEKIIILSSLEEEIPEDESLNVATDCLEKEETVDHTILVPTMENEAHYYDFHIVYSASYKVPVLYFRGYCSGGEPLALDVIKKDVPSCSVSLLLESKWTFITQEEHPYLNRPWFKLHPCGTEDWIKLLSQSSSSSGCQMPIVLYLVSWFSVVGQVVGLRIPLEMLN.

Reference

Reference1. Phillips, A. R., Suttangkakul, A., & Vierstra, R. D. (2008). The ATG12-conjugating enzyme ATG10 is essential for autophagic vesicle formation in Arabidopsis thaliana. Genetics, 178(3), 1339-1353.
2. Avin-Wittenberg, T., Honig, A., & Galili, G. (2012). Variations on a theme: plant autophagy in comparison to yeast and mammals. Protoplasma, 249, 285-299.
3. Avin-Wittenberg, T., Honig, A., & Galili, G. (2012). Variations on a theme: plant autophagy in comparison to yeast and mammals. Protoplasma, 249, 285-299.

Figure 1 Enhanced sensitivity of atg10-1 plants to N- and C-limiting conditions. Lines include wild-type Col-0 (WT) and homozygous atg5-1, atg7-1, and atg10-1 mutants. Survival on N-deficient medium. One-week-old seedlings were sown on N-rich solid medium and transferred to N-deficient medium for various lengths of time before transfer back to N-rich medium, all under SD. The graph plots the percentage of plants that resumed growth after exposure to N-deficient medium. Each point represents the analysis of 45 seedlings.
Reference: Phillips, A. R., Suttangkakul, A., & Vierstra, R. D. (2008). The ATG12-conjugating enzyme ATG10 is essential for autophagic vesicle formation in Arabidopsis thaliana. Genetics, 178(3), 1339-1353.

For Research Use Only | Not For Clinical Use.
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