Mouse Anti-Arabidopsis ATG10 Antibody (CBMOAB-24499FYC)
Cat: CBMOAB-24499FYC
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Specifications
Host species | Mouse (Mus musculus) |
Species Reactivity | A. thaliana (Arabidopsis thaliana) |
Clone | MO24499FC |
Specificity | This antibody binds to Arabidopsis ATG10. |
Format | Liquid or Lyophilized |
Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
Purity | > 90% was determined by SDS-PAGE |
Purification | Purified with Protein A or G affinity chromatography |
Application Information
Application | WB, ELISA |
Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
Introduction | Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003). |
Product Overview | Mouse Anti-Arabidopsis ATG10 Antibody is a mouse antibody against ATG10. It can be used for ATG10 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
Alternative Names | Autophagy Related 10; Autophagy-Related Protein 10; APG10L; ATG10 Autophagy Related 10 Homolog (S. Cerevisiae); APG10 Autophagy 10-Like (S. Cerevisiae); Ubiquitin-Like-Conjugating Enzyme ATG10; ATG10 Autophagy Related 10 Homolog |
UniProt ID | Q8VZ52 |
Protein Refseq | The length of the protein is 225 amino acids long. The sequence is show below: MDSAREVSDGRLTVEGFSVASRAFADKWKIHNQSFPPWSWVPLINRTLLVSKKEEGYLSLEKIIILSSLEEEIPEDESLNVATDCLEKEETVDHTILVPTMENEAHYYDFHIVYSASYKVPVLYFRGYCSGGEPLALDVIKKDVPSCSVSLLLESKWTFITQEEHPYLNRPWFKLHPCGTEDWIKLLSQSSSSSGCQMPIVLYLVSWFSVVGQVVGLRIPLEMLN. |
Reference
Reference | 1. Phillips, A. R., Suttangkakul, A., & Vierstra, R. D. (2008). The ATG12-conjugating enzyme ATG10 is essential for autophagic vesicle formation in Arabidopsis thaliana. Genetics, 178(3), 1339-1353. 2. Avin-Wittenberg, T., Honig, A., & Galili, G. (2012). Variations on a theme: plant autophagy in comparison to yeast and mammals. Protoplasma, 249, 285-299. 3. Avin-Wittenberg, T., Honig, A., & Galili, G. (2012). Variations on a theme: plant autophagy in comparison to yeast and mammals. Protoplasma, 249, 285-299. |
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Figure 1 Enhanced sensitivity of atg10-1 plants to N- and C-limiting conditions. Lines include wild-type Col-0 (WT) and homozygous atg5-1, atg7-1, and atg10-1 mutants. Survival on N-deficient medium. One-week-old seedlings were sown on N-rich solid medium and transferred to N-deficient medium for various lengths of time before transfer back to N-rich medium, all under SD. The graph plots the percentage of plants that resumed growth after exposure to N-deficient medium. Each point represents the analysis of 45 seedlings.
Reference: Phillips, A. R., Suttangkakul, A., & Vierstra, R. D. (2008). The ATG12-conjugating enzyme ATG10 is essential for autophagic vesicle formation in Arabidopsis thaliana. Genetics, 178(3), 1339-1353.
For Research Use Only | Not For Clinical Use.
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