Mouse Anti-ATG10 Antibody (CBMOAB-24499FYC)

Cat: CBMOAB-24499FYC

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Product List
Specifications
Application Information
Target
Reference
Relate Reference Data

Sub Cat	Clonality	Species Reactivity	Application	Clone	Conjugate	Size
CBMOAB-24499FYC	Monoclonal	A. thaliana (Arabidopsis thaliana), C. elegans (Caenorhabditis elegans), Cattle (Bos taurus), Chimpanzee (Pan troglodytes), Fruit fly (Drosophila melanogaster), Horse (Equus caballus), Maize (Zea mays), Pig (Sus scrofa), Rat (Rattus norvegicus), Yeast, Zebrafish (Danio rerio)	WB, ELISA	MO24499FC		100 µg	Inquiry
Protein Refseq: The length of the protein is 225 amino acids long. The sequence is show below: MDSAREVSDGRLTVEGFSVASRAFADKWKIHNQSFPPWSWVPLINRTLLVSKKEEGYLSLEKIIILSSLEEEIPEDESLNVATDCLEKEETVDHTILVPTMENEAHYYDFHIVYSASYKVPVLYFRGYCSGGEPLALDVIKKDVPSCSVSLLLESKWTFITQEEHPYLNRPWFKLHPCGTEDWIKLLSQSSSSSGCQMPIVLYLVSWFSVVGQVVGLRIPLEMLN.
CBMOAB-01983FYA	Monoclonal	Fruit fly (Drosophila melanogaster)	WB, ELISA	MO01983FYA		100 µg	Inquiry
Protein Refseq: The length of the protein is 212 amino acids long. The sequence is show below: MYEACFPRKRNCFLSSIVTRGYLKCLKILTRIFLCFIFSIMGDLSWKDFLSQAKQFLEISQKLGDNWILEQKDSNEPNTYLKCSQKIKCRGGKDNSAELVSVEYHVVFSVSYQVPMLFFQAHRSDGSLLDVEATWRMFMPESKASDLHQILTQMDHPVLFRPFMALHPCRTAEVLKQFGKPSCNQVLTFISLYGPHVQLHLQNAYGLSQEYT.
CBMOAB-66900FYA	Monoclonal	Zebrafish (Danio rerio)	WB, ELISA	MO66900FYA		100 µg	Inquiry
Protein Refseq: The length of the protein is 224 amino acids long. The sequence is show below: MMTGERKPASCFLDENTFRLCCRLFLQHSESIQDGWIWEQIKGSDEGFMKKTVLIPVKSSLLDKQHESIQAENTELPTDDFEADAEDETAGVDAVCESHAVLRYEYHVLYSCSYQIPVLYFRASALDGRSLSLEEVWSNVHPNYRQRLKQEPWDTLTQQEHPLLGQPFFMLHPCRTEEFMKPALELAHAQNRRVNYIVSWLSVVGPVVGLDVPLSFSTAVSAPD.
CBMOAB-00367CR	Monoclonal	Yeast	WB, ELISA	MO00367CR		100 µg	Inquiry
Protein Refseq: The length of the protein is 167 amino acids long. The sequence is show below: MIPYQEWHSQLQSLYDSQIFHNWALCQDVHLNDEKDGLLLRLIPTRQLQKNTERIENKLLNHIELYLTYSKVYNEPLLLLRIWEEKSIDGIPMTKLMLPTDIESLLDVQGKFQLGLDTIINLEGSVWYSFHPCDTSCIVGDQAEFMSTYLRRWVSIFIFSWLGYEDS.
CBMOAB-00740HCB	Monoclonal	C. elegans (Caenorhabditis elegans)	WB, ELISA	MO00740HB		100 µg	Inquiry
Protein Refseq: The length of the protein is 157 amino acids long. The sequence is show below: MLTEQQFRDEIRGFVDKMNENNFHWQLKEIGKYARETHLKTLSDGRVITSETHILYNSTYQVPTIWFNFFENNGSPLPFRTVIRDVLNISETEESEASIRSRISHYEHPFMGVLYYNIHPCNTSNIMKELNTDRSYLMSWFSVYGQQIGLKLPDRVL.
MO-AB-15789W	Monoclonal	Chimpanzee (Pan troglodytes)	WB, ELISA	MO15789W		100 µg	Inquiry
Protein Refseq: The length of the protein is 220 amino acids long. The sequence is show below: MEEDEFIGEKTFQRYCAEFIKHSQQIGDSWEWRPSKDCSDGYMCKIHFQIKNGSVMSHLGASTHGQTCLPMEEAFELPLDDCEVIETAAASEVIKYEYHVLYSCSHQVPVLYFRASFLDGRPLTLKDIWEGVHECYKMRLLQGPWDTITQQEHPILGQPFFVLHPCKTNEFMTPVLKNSQKINKNVNYITSWLSIVGPVVGLNLPLSYAKATSQDERNVH.
MO-AB-43751W	Monoclonal	Horse (Equus caballus)	WB, ELISA	MO43751W		100 µg	Inquiry
Protein Refseq: The length of the protein is216 amino acids long. The sequence is show below: MEENEFFGEKTFQRYCAEFIKHSQQIGDGWEWRTSKDSSDGYMCKMHFQIKNETVVSRAGTSAHEQTCLPMEEALELPLDDFEVTESTATNEVIKYEYHVLYSCSYQVPVLYFRASFLDGRPLALKDIWEGVHECYKTRLLQGPWDTISQQEHPILGQPFFVLHPCKTNEFMTPVLKNSRKINRNVNYITSWLSLVGPVVGLHLPLSYAGAASQDE.
MO-AB-47411W	Monoclonal	Maize (Zea mays)	WB, ELISA	MO47411W		100 µg	Inquiry
Protein Refseq: The length of the protein is215 amino acids long. The sequence is show below: MGGSPVWDGTLSPDSFNTSAAALVKRWKEIEVDDSLPEWTWKPCSKLGVPSEVEGYLALERVYRSCGTSQEQIEEMKFDGAGDVAWVESSSDNVHVYDFHAVYSFSYKVPVLYFRGYHTSGQLLTLDEVKEGLPSHSQKVLSESKWTFITQEEHPHLSKPWLALHPCATSGWMKLLLEETEATDKEHSVQYLPAWLSVVGQAVGLKIPLELHSNS.
MO-AB-07745R	Monoclonal	Cattle (Bos taurus)	WB, ELISA	MO07745R		100 µg	Inquiry
Protein Refseq: The length of the protein is 234 amino acids long. The sequence is show below: MEENEFFGEKTFQHYCAEFIKHSQKIGDGWEWRASKDCSDGYMCKTHFQVKNGTAVSHQGTSDHVQTFLPVEEALDPPLDDLEVNETTTAAEVIKYEYHVLYSCSYQVPVLYFRASFLDGRPLALKDIWEGVHECYKMRLLQGPWDTITQQEHPILGQPFFVLHPCKTSEFMTPVLKNSQKINRNVNYITSWLSMVGPAVGLNLPLSYATAASQDEGNVDGQDSSIERKSLEEH.
MO-AB-23905R	Monoclonal	Pig (Sus scrofa)	WB, ELISA	MO23905R		100 µg	Inquiry
Protein Refseq: The length of the protein is 174 amino acids long. The sequence is show below: MEEDEFFGEKTFQRCCAEFIKHSQQIGDGWEWRTSKDCSDGYMCKTHFQVKNGTAVSHPETSAHVQTCLPMEEALEPPLDDSEVTETDTATEVIKYEYHVLYSCSYQVPVLYFRASFLDGKPLALKDLWERVHECYKTRLLQGPWDTITQQVGEYFHCSICDSSLLLIFSFFSL.
MO-AB-24213H	Monoclonal	Rat (Rattus norvegicus)	WB, ELISA	MO24213C		100 µg	Inquiry
Protein Refseq: The length of the protein is 214 amino acids long. The sequence is show below: MEDEFFGEESFQHCCAEFIRHSQQIGDGWEWRTAKECSDGYMCKTQFQIKKETPTPHRETPASVHTCLPTEENLELPVDDSEAVGPAAAAVIRQEYHVLYSCSYQVPVLYFRASFLDGRPLALEDIWEGVHECYKLRLLQGPWDTITQQEHPILGQPFFVLHPCKTNEFMTAVLKNSQKINRNVNYITSWLSIVGPVVGLNLPLSYAKATSQSE.

Specifications

Host species	Mouse (Mus musculus)
Species Reactivity	A. thaliana (Arabidopsis thaliana), C. elegans (Caenorhabditis elegans), Cattle (Bos taurus), Chimpanzee (Pan troglodytes), Fruit fly (Drosophila melanogaster), Horse (Equus caballus), Maize (Zea mays), Pig (Sus scrofa), Rat (Rattus norvegicus), Yeast, Zebrafish (Danio rerio)
Clone	MO24499FC
Specificity	This antibody binds to Arabidopsis ATG10.
Format	Liquid or Lyophilized
Storage	Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use
Purity	> 90% was determined by SDS-PAGE
Purification	Purified with Protein A or G affinity chromatography

Application Information

Application	WB, ELISA
Application Notes	ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

Introduction	Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003).
Product Overview	Mouse Anti-Arabidopsis ATG10 Antibody is a mouse antibody against ATG10. It can be used for ATG10 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative Names	Autophagy Related 10; Autophagy-Related Protein 10; APG10L; ATG10 Autophagy Related 10 Homolog (S. Cerevisiae); APG10 Autophagy 10-Like (S. Cerevisiae); Ubiquitin-Like-Conjugating Enzyme ATG10; ATG10 Autophagy Related 10 Homolog
UniProt ID	Q8VZ52
Protein Refseq	The length of the protein is 225 amino acids long. The sequence is show below: MDSAREVSDGRLTVEGFSVASRAFADKWKIHNQSFPPWSWVPLINRTLLVSKKEEGYLSLEKIIILSSLEEEIPEDESLNVATDCLEKEETVDHTILVPTMENEAHYYDFHIVYSASYKVPVLYFRGYCSGGEPLALDVIKKDVPSCSVSLLLESKWTFITQEEHPYLNRPWFKLHPCGTEDWIKLLSQSSSSSGCQMPIVLYLVSWFSVVGQVVGLRIPLEMLN.

Reference

1. Phillips, A. R., Suttangkakul, A., & Vierstra, R. D. (2008). The ATG12-conjugating enzyme ATG10 is essential for autophagic vesicle formation in Arabidopsis thaliana. Genetics, 178(3), 1339-1353.
2. Avin-Wittenberg, T., Honig, A., & Galili, G. (2012). Variations on a theme: plant autophagy in comparison to yeast and mammals. Protoplasma, 249, 285-299.
3. Avin-Wittenberg, T., Honig, A., & Galili, G. (2012). Variations on a theme: plant autophagy in comparison to yeast and mammals. Protoplasma, 249, 285-299.

Relate Reference Data

Figure 1 Enhanced sensitivity of atg10-1 plants to N- and C-limiting conditions. Lines include wild-type Col-0 (WT) and homozygous atg5-1, atg7-1, and atg10-1 mutants. Survival on N-deficient medium. One-week-old seedlings were sown on N-rich solid medium and transferred to N-deficient medium for various lengths of time before transfer back to N-rich medium, all under SD. The graph plots the percentage of plants that resumed growth after exposure to N-deficient medium. Each point represents the analysis of 45 seedlings.
Reference: Phillips, A. R., Suttangkakul, A., & Vierstra, R. D. (2008). The ATG12-conjugating enzyme ATG10 is essential for autophagic vesicle formation in Arabidopsis thaliana. Genetics, 178(3), 1339-1353.

For Research Use Only | Not For Clinical Use.

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