Mouse Anti-Arabidopsis RAD50 Antibody (CBMOAB-39755FYC)
Cat: CBMOAB-39755FYC
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- Product Details
- Relate Reference Data
Specifications
Host species | Mouse (Mus musculus) |
Species Reactivity | A. thaliana (Arabidopsis thaliana) |
Clone | MO39755FC |
Specificity | This antibody binds to Arabidopsis RAD50. |
Format | Liquid or Lyophilized |
Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
Purity | > 90% was determined by SDS-PAGE |
Purification | Purified with Protein A or G affinity chromatography |
Cellular Localization | Nucleus; Other locations |
Application Information
Application | WB, ELISA |
Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
Introduction | The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder. |
Product Overview | Mouse Anti-Arabidopsis RAD50 (clone MO39755FC) Antibody (CBMOAB-39755FYC) is a mouse antibody against RAD50. It can be used for RAD50 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
Alternative Names | RAD50 Double Strand Break Repair Protein; RAD50 Homolog, Double Strand Break Repair Protein; HRad50; RAD50 (S. Cerevisiae) Homolog; RAD50 Homolog (S. Cerevisiae); DNA Repair Protein RAD50; EC 3.6.3.27 |
UniProt ID | Q9SL02 |
Protein Refseq | The length of the protein is 1316 amino acids long. The sequence is show below: MSTVDKMLIKGIRSFDPENKNVVTFFRPLTLIVGANGAGKTTIIECLKVSCTGELPPNARSGHSFIHDPKVAGETETKAQIKLRFKTAAGKDVVCIRSFQLTQKASKMEYKAIESVLQTINPHTGEKVCLSYRCADMDREIPALMGVSKAILENVIFVHQDESNWPLQDPSTLKKKFDDIFSATRYTKALEVIKKLHKDQAQEIKTFKLKLENLQTLKDAAYKLRESIAQDQERTESSKVQMLELETSVQKVDAEVHNKEMMLKDLRKLQDQVSIKTAERSTLFKEQQRQYAALPEENEDTIEELKEWKSKFEERLALLGTKIRKMEREMVDTETTISSLHNAKTNYMLEISKLQTEAEAHMLLKNERDSTIQNIFFHYNLGNVPSTPFSTEVVLNLTNRIKSRLGELEMDLLDKKKSNETALSTAWDCYMDANDRWKSIEAQKRAKDEIKMGISKRIEEKEIERDSFEFEISTVDVKQTDEREKQVQVELERKTKQNSERGFESKIEQKQHEIYSLEHKIKTLNRERDVMAGDAEDRVKLSLKKTEQENLKKKHKKIIDECKDRIRGVLKGRLPPEKDMKREIVQALRSIEREYDDLSLKSREAEKEVNMLQMKIQEVNNSLFKHNKDTESRKRYIESKLQALKQESVTIDAYPKLLESAKDKRDDRKREYNMANGMRQMFEPFEKRARQEHSCPCCERSFTADEEASFIKKQRVKASSTGEHLKALAVESSNADSVFQQLDKLRAVFEEYSKLTTEIIPLAEKTLQEHTEELGQKSEALDDVLGISAQIKADKDSIEALVQPLENADRIFQEIVSYQKQIEDLEYKLDFRGLGVKTMEEIQSELSSLQSSKDKLHGELEKLRDDQIYMERDISCLQARWHAVREEKAKAANLLRDVTKAEEDLERLAEEKSQLDLDVKYLTEALGPLSKEKEQLLSDYNDMKIRRNQEYEELAEKKRNYQQEVEALLKASYKINEYHDLKKGERLDDIQEKQRLSDSQLQSCEARKNELAGELNRNKDLMRNQDQLRRNIEDNLNYRTTKAKVEELTREIESLEEQILNIGGIAAVEAEIVKILRERERLLSELNRCRGTVSVYESSISKNRVELKQAQYKDIDKRHFDQLIQLKTTEMANKDLDRYYNALDKALMRFHTMKMEEINKIIRELWQQTYRGQDMDYIRIHSDSEGAGTRSYSYKVLMQTGDTELEMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDGPNSESLAGALLRIMEDRKGQENFQLIVITHDERFAQMIGQRQHAEKYYRVAKDDMQHSIIEAQEIFD. |
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IP
Figure 1 Co-immunoprecipitation of Rad50 and Mre11 in a: T87 suspension cells and b: Arabidopsis flowers buds. Immunoprecipitates from T87 suspension cells and from flower buds of wild-type plants (+/+) and plants homozygous for the mutation of RAD50 (−/−) were analyzed in Western blot with the anti-Rad50 antiserum (304) and the anti-Mre11 antibody. Sera used in the immunoprecipitation experiments are indicated above each panel. Pre-immune sera (PI) were used as controls. Mre11, affinity-purified anti-Mre11 antibody; I98, antiserum 98 anti-Rad50; non, protein extracts analyzed in Western blot without immunoprecipitation experiments before. IgGs, heavy chain of gammaglobulins.
For Research Use Only | Not For Clinical Use.
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