Mouse Anti-RAD50 Antibody (CBMOAB-39755FYC)


Cat: CBMOAB-39755FYC
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Sub Cat Clonality Species Reactivity Application Clone Conjugate Size  
CBMOAB-39755FYC Monoclonal A. thaliana (Arabidopsis thaliana), C. elegans (Caenorhabditis elegans), Chicken (Gallus gallus), Chimpanzee (Pan troglodytes), Frog (Xenopus laevis), Fruit fly (Drosophila melanogaster), Marmoset, O. mykiss (Oncorhynchus mykiss), Rabbit (Oryctolagus cuniculus), Rhesus (Macaca mulatta), Rice (Oryza), Yeast, Zebrafish (Danio rerio) WB, ELISA MO39755FC 100 µg
CBMOAB-28724FYA Monoclonal Fruit fly (Drosophila melanogaster) WB, ELISA MO28724FYA 100 µg
CBMOAB-55964FYA Monoclonal Rhesus (Macaca mulatta) WB, ELISA MO55964FYA 100 µg
CBMOAB-95138FYA Monoclonal Zebrafish (Danio rerio) WB, ELISA MO95138FYA 100 µg
CBMOAB-03265CR Monoclonal Yeast WB, ELISA MO03265CR 100 µg
CBMOAB-08977HCB Monoclonal C. elegans (Caenorhabditis elegans) WB, ELISA MO08977HB 100 µg
CBMOAB-89009FYB Monoclonal Rice (Oryza) WB, ELISA MO89009FYB 100 µg
MO-AB-24456W Monoclonal Chimpanzee (Pan troglodytes) WB, ELISA MO24456W 100 µg
MO-AB-62874W Monoclonal Marmoset WB, ELISA MO62874W 100 µg
MO-AB-06871H Monoclonal Frog (Xenopus laevis) WB, ELISA MO06871C 100 µg
MO-AB-03673Y Monoclonal Chicken (Gallus gallus) WB, ELISA MO03673Y 100 µg
MO-AB-09634Y Monoclonal Rabbit (Oryctolagus cuniculus) WB, ELISA MO09634Y 100 µg
MO-AB-12940Y Monoclonal O. mykiss (Oncorhynchus mykiss) WB, ELISA MO12940Y 100 µg

Specifications

Host speciesMouse (Mus musculus)
Species ReactivityA. thaliana (Arabidopsis thaliana), C. elegans (Caenorhabditis elegans), Chicken (Gallus gallus), Chimpanzee (Pan troglodytes), Frog (Xenopus laevis), Fruit fly (Drosophila melanogaster), Marmoset, O. mykiss (Oncorhynchus mykiss), Rabbit (Oryctolagus cuniculus), Rhesus (Macaca mulatta), Rice (Oryza), Yeast, Zebrafish (Danio rerio)
CloneMO39755FC
SpecificityThis antibody binds to Arabidopsis RAD50.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationNucleus; Other locations

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionThe protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Product OverviewMouse Anti-Arabidopsis RAD50 (clone MO39755FC) Antibody (CBMOAB-39755FYC) is a mouse antibody against RAD50. It can be used for RAD50 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesRAD50 Double Strand Break Repair Protein; RAD50 Homolog, Double Strand Break Repair Protein; HRad50; RAD50 (S. Cerevisiae) Homolog; RAD50 Homolog (S. Cerevisiae); DNA Repair Protein RAD50; EC 3.6.3.27
UniProt IDQ9SL02
Protein RefseqThe length of the protein is 1316 amino acids long. The sequence is show below: MSTVDKMLIKGIRSFDPENKNVVTFFRPLTLIVGANGAGKTTIIECLKVSCTGELPPNARSGHSFIHDPKVAGETETKAQIKLRFKTAAGKDVVCIRSFQLTQKASKMEYKAIESVLQTINPHTGEKVCLSYRCADMDREIPALMGVSKAILENVIFVHQDESNWPLQDPSTLKKKFDDIFSATRYTKALEVIKKLHKDQAQEIKTFKLKLENLQTLKDAAYKLRESIAQDQERTESSKVQMLELETSVQKVDAEVHNKEMMLKDLRKLQDQVSIKTAERSTLFKEQQRQYAALPEENEDTIEELKEWKSKFEERLALLGTKIRKMEREMVDTETTISSLHNAKTNYMLEISKLQTEAEAHMLLKNERDSTIQNIFFHYNLGNVPSTPFSTEVVLNLTNRIKSRLGELEMDLLDKKKSNETALSTAWDCYMDANDRWKSIEAQKRAKDEIKMGISKRIEEKEIERDSFEFEISTVDVKQTDEREKQVQVELERKTKQNSERGFESKIEQKQHEIYSLEHKIKTLNRERDVMAGDAEDRVKLSLKKTEQENLKKKHKKIIDECKDRIRGVLKGRLPPEKDMKREIVQALRSIEREYDDLSLKSREAEKEVNMLQMKIQEVNNSLFKHNKDTESRKRYIESKLQALKQESVTIDAYPKLLESAKDKRDDRKREYNMANGMRQMFEPFEKRARQEHSCPCCERSFTADEEASFIKKQRVKASSTGEHLKALAVESSNADSVFQQLDKLRAVFEEYSKLTTEIIPLAEKTLQEHTEELGQKSEALDDVLGISAQIKADKDSIEALVQPLENADRIFQEIVSYQKQIEDLEYKLDFRGLGVKTMEEIQSELSSLQSSKDKLHGELEKLRDDQIYMERDISCLQARWHAVREEKAKAANLLRDVTKAEEDLERLAEEKSQLDLDVKYLTEALGPLSKEKEQLLSDYNDMKIRRNQEYEELAEKKRNYQQEVEALLKASYKINEYHDLKKGERLDDIQEKQRLSDSQLQSCEARKNELAGELNRNKDLMRNQDQLRRNIEDNLNYRTTKAKVEELTREIESLEEQILNIGGIAAVEAEIVKILRERERLLSELNRCRGTVSVYESSISKNRVELKQAQYKDIDKRHFDQLIQLKTTEMANKDLDRYYNALDKALMRFHTMKMEEINKIIRELWQQTYRGQDMDYIRIHSDSEGAGTRSYSYKVLMQTGDTELEMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDGPNSESLAGALLRIMEDRKGQENFQLIVITHDERFAQMIGQRQHAEKYYRVAKDDMQHSIIEAQEIFD.

Relate Reference Data

IP

Figure 1 Co-immunoprecipitation of Rad50 and Mre11 in a: T87 suspension cells and b: Arabidopsis flowers buds. Immunoprecipitates from T87 suspension cells and from flower buds of wild-type plants (+/+) and plants homozygous for the mutation of RAD50 (−/−) were analyzed in Western blot with the anti-Rad50 antiserum (304) and the anti-Mre11 antibody. Sera used in the immunoprecipitation experiments are indicated above each panel. Pre-immune sera (PI) were used as controls. Mre11, affinity-purified anti-Mre11 antibody; I98, antiserum 98 anti-Rad50; non, protein extracts analyzed in Western blot without immunoprecipitation experiments before. IgGs, heavy chain of gammaglobulins.

For Research Use Only | Not For Clinical Use.
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