Mouse Anti-MAD2 Antibody (CBMOAB-02133CR)
Cat: CBMOAB-02133CR
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
- Product List
- Specifications
- Application Information
- Target
| Sub Cat | Clonality | Species Reactivity | Application | Clone | Conjugate | Size | |
| CBMOAB-02133CR | Monoclonal | Yeast, A. thaliana (Arabidopsis thaliana), Frog (Xenopus laevis), Fruit fly (Drosophila melanogaster), Medaka (Oryzias latipes), S. pombe | WB, ELISA | MO02133CR | 100 µg | ||
| CBMOAB-23390FYA | Monoclonal | Fruit fly (Drosophila melanogaster) | WB, ELISA | MO23390FYA | 100 µg | ||
| CBMOAB-36126FYC | Monoclonal | A. thaliana (Arabidopsis thaliana) | WB, ELISA | MO36126FC | 100 µg | ||
| MO-AB-00855R | Monoclonal | Medaka (Oryzias latipes) | WB, ELISA | MO00855R | 100 µg | ||
| MO-AB-04966H | Monoclonal | Frog (Xenopus laevis) | WB, ELISA | MO04966C | 100 µg | ||
| MOFY-0922-FY27 | Monoclonal | S. pombe | WB, IP, IF, ELISA | MC-3 | 100 µg |
Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | Yeast, A. thaliana (Arabidopsis thaliana), Frog (Xenopus laevis), Fruit fly (Drosophila melanogaster), Medaka (Oryzias latipes), S. pombe |
| Clone | MO02133CR |
| Specificity | This antibody binds to Yeast MAD2. |
| Format | Liquid or Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
| Cellular Localization | Nucleus; Other locations |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Introduction | Central component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Thought to be recruited to unattached kinetochores by MAD1. During checkpoint activity, MAD2 is relayed from the MAD1-MAD2 complex to the mitotic checkpoint complex (MCC). MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20. |
| Product Overview | Mouse Anti-Yeast MAD2 Antibody is a mouse antibody against MAD2. It can be used for MAD2 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | Mitotic spindle checkpoint component MAD2; Mitotic MAD2 protein; MAD2; YJL030W |
| UniProt ID | P40958 |
| Protein Refseq | The length of the protein is 196 amino acids long. The sequence is show below: MSQSISLKGSTRTVTEFFEYSINSILYQRGVYPAEDFVTVKKYDLTLLKTHDDELKDYIRKILLQVHRWLLGGKCNQLVLCIVDKDEGEVVERWSFNVQHISGNSNGQDDVVDLNTTQSQIRALIRQITSSVTFLPELTKEGGYTFTVLAYTDADAKVPLEWADSNSKEIPDGEVVQFKTFSTNDHKVGAQVSYKY. |
For Research Use Only | Not For Clinical Use.
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