AibGenesis™ Mouse Anti-MLS1 Antibody (CBMOAB-02315CR)


Cat: CBMOAB-02315CR

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Lot Number

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Sub Cat Clonality Species Reactivity Application Clone Conjugate Size  
CBMOAB-02315CR Monoclonal Yeast, C. elegans (Caenorhabditis elegans) WB, ELISA MO02315CR 100 µg
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CBMOAB-06666HCB Monoclonal C. elegans (Caenorhabditis elegans) WB, ELISA MO06666HB 100 µg
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityYeast, C. elegans (Caenorhabditis elegans)
CloneMO02315CR
SpecificityThis antibody binds to Yeast MLS1.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationOther locations; Peroxisome; Cytosol

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionMalate synthase which takes part in the glyoxylate cycle (PubMed:1454530). MLS1 activity is essential for cells to grow on oleic acid as a sole carbon source (PubMed:11846793). Two steps of the glyoxylate cycle take place in the cytosol, the splitting of isocitrate into succinate and glyoxylate, and the dehydrogenation of malate to oxaloacetate (PubMed:1454530). However, the formation of malate from glyoxylate and acetyl-CoA undertaken MLS1, occurs in the peroxisomes when cells are grown on oleic acid (Probable). The source of acetyl-CoA being either peroxisomal when breaking down fatty acids, or cytosolic when extra-cellular two-carbon substrates are used, therefore, although not strictly essential, the peroxisomal localization of MLS1 appears to be advantageous for cells growing on oleic acid, in that acetyl-CoA production and utilization are thereby intimately compartmentalized together to increase efficiency (Probable). (From uniprot, under CC BY 4.0)
Product OverviewMouse Anti-Yeast MLS1 Antibody is a mouse antibody against MLS1. It can be used for MLS1 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesMalate synthase 1, glyoxysomal; EC 2.3.3.9; MLS1; YNL117W
UniProt IDP30952
Protein RefseqThe length of the protein is 554 amino acids long. The sequence is show below: MVKVSLDNVKLLVDVDKEPFFKPSSTTVGDILTKDALEFIVLLHRTFNNKRKQLLENRQVVQKKLDSGSYHLDFLPETANIRNDPTWQGPILAPGLINRSTEITGPPLRNMLINALNAPVNTYMTDFEDSASPTWNNMVYGQVNLYDAIRNQIDFDTPRKSYKLNGNVANLPTIIVRPRGWHMVEKHLYVDDEPISASIFDFGLYFYHNAKELIKLGKGPYFYLPKMEHHLEAKLWNDVFCVAQDYIGIPRGTIRATVLIETLPAAFQMEEIIYQLRQHSSGLNCGRWDYIFSTIKRLRNDPNHILPNRNQVTMTSPFMDAYVKRLINTCHRRGVHAMGGMAAQIPIKDDPAANEKAMTKVRNDKIRELTNGHDGSWVAHPALAPICNEVFINMGTPNQIYFIPENVVTAANLLETKIPNGEITTEGIVQNLDIGLQYMEAWLRGSGCVPINNLMEDAATAEVSRCQLYQWVKHGVTLKDTGEKVTPELTEKILKEQVERLSKASPLGDKNKFALAAKYFLPEIRGEKFSEFLTTLLYDEIVSTKATPTDLSKL.
For Research Use Only | Not For Clinical Use.
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