Mouse Anti-birA Antibody (CBMOAB-0278YC)


Cat: CBMOAB-0278YC
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Lot Number

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  • Specifications
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Sub Cat Clonality Species Reactivity Application Clone Conjugate Size  
CBMOAB-0278YC Monoclonal E. coli (Escherichia coli ), E. coli (Escherichia coli), Yeast WB, ELISA MO0278YC 100 µg
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MO-NAB-00145W Monoclonal E. coli (Escherichia coli) WB, IF NW0191 100 µg
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MO-NAB-00146W Monoclonal E. coli (Escherichia coli), Yeast WB, IF 6C4C7 100 µg
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MO-DKB-00576W Polyclonal E. coli (Escherichia coli) ELISA 100 µg
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityE. coli (Escherichia coli ), E. coli (Escherichia coli), Yeast
CloneMO0278YC
SpecificityThis antibody binds to Escherichia coli K-12 birA.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionActs both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
Product OverviewMouse Anti-E. coli birA Antibody is a mouse antibody against birA. It can be used for birA detection in Western Blot and Enzyme-Linked Immunosorbent Assay.
Alternative NamesBifunctional ligase/repressor BirA; Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] ligase; EC 6.3.4.15; Biotin--protein ligase; Biotin-[acetyl-CoA carboxylase] synthetase; birA; bioR dhbB; b3973 JW3941
UniProt IDP06709
Protein RefseqThe length of the protein is 321 amino acids long. The sequence is show below: MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK.
For Research Use Only | Not For Clinical Use.
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