Mouse Anti-E. coli glyA Antibody (CBMOAB-1056YC)


Cat: CBMOAB-1056YC
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityE. coli (Escherichia coli )
CloneMO1056YC
SpecificityThis antibody binds to Escherichia coli K-12 glyA.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionCatalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. Thus, is able to catalyze the cleavage of allothreonine and 3-phenylserine. Also catalyzes the irreversible conversion of 5,10-methenyltetrahydrofolate to 5-formyltetrahydrofolate.
Product OverviewMouse Anti-E. coli glyA (clone MO1056YC) Antibody (CBMOAB-1056YC) is a mouse antibody against glyA. It can be used for glyA detection in Western Blot and Enzyme-Linked Immunosorbent Assay.
Alternative NamesSerine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1; glyA; b2551 JW2535
UniProt IDP0A825
Protein RefseqThe length of the protein is 417 amino acids long. The sequence is show below: MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQANFAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDSINDEAVIERIKGKVLDICARYPVYA.

ELISA

Figure 1 Mouse Anti-E. coli glyA (clone MO1056YC) Antibody CBMOAB-1056YC in ELISA.
ELISA analysis of Ascite of CBMOAB-1056YC was performed by coating with recombinant E. coli glyA antigen peptide.
The abscissa represents the dilution fold (*10k).

For Research Use Only | Not For Clinical Use.
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