Mouse Anti-MED25 Antibody (CBMOAB-36353FYC)

Sub Cat	Clonality	Species Reactivity	Application	Clone	Conjugate	Size
CBMOAB-36353FYC	Monoclonal	A. thaliana (Arabidopsis thaliana), Arabidopsis, Cattle (Bos taurus), Fruit fly (Drosophila melanogaster), Marmoset, Rhesus (Macaca mulatta), Zebrafish (Danio rerio)	WB, ELISA	MO36353FC		100 µg	Inquiry
Protein Refseq: The length of the protein is 836 amino acids long. The sequence is show below: MSSEVKQLIVVAEGTAALGPYWQTIVSDYLEKIIRSFCGSELNGERNPVSTVELSLVIFNSHGSYCACLVQRSGWTRDVDIFLHWLSSIQFGGGGFNEVATAEGLAEALMMFSPPSGQAQPSNDLKRHCILITASNPHILPTPVYRPRLQNVERNENGDAQAESRLSDAETVASYFAKCSVSLSVVCPKQLPTIRALYNAGKPNQQSADLSIDTAKNTFYLVLISENFVEACAALSHSATNLPQTQSPVKVDRATVAPSIPVTGQPPAPVSSANGPIQNRQPVSVGPVPTATVKVEPSTVTSMAPVPSFPHIPAVARPATQAIPSIQTSSASPVSQDMVSNAENAPDIKPVVVSGMTPPLRTGPPGGANVNLLNNLSQVRQVMSSAALAGAASSVGQSAVAMHMSNMISTGMATSLPPSQTVFSTGQQGITSMAGSGALMGSAQTGQSPGPNNAFSPQTTSNVASNLGVSQPMQGMNQGSHSGAMMQGGISMNQNMMSGLGQGNVSSGTGGMMPTPGVGQQAQSGIQQLGGSNSSAPNMQLSQPSSGAMQTSQSKYVKVWEGNLSGQRQGQPVLITRLEGYRSASASDSLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMSQHGFLGQLQDKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIPNQQQQQQQQLHQQQQQQQQIQQQQQQQQHLQQQQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQQQQQQQHQLTQLQHHHQQQQQASPLNQMQQQTSPLNQMQQQTSPLNQMQQQQQPQQMVMGGQAFAQAPGRSQQGGGGGQPNMPGAGFMG.
CBMOAB-23584FYA	Monoclonal	Fruit fly (Drosophila melanogaster)	WB, ELISA	MO23584FYA		100 µg	Inquiry
Protein Refseq: The length of the protein is 863 amino acids long. The sequence is show below: MEVDQIPLADVVFVIEGSAINGAYINELKTNYILPTLEHFTTGSIDEREYLIAERFATLYGIVVYRTAANLLEPVCSTYGPFLQPQKVMETIERLPLVGGGMESCAHMAEGFAAAHGCFDDISERRQLLDQTSVQRHCILICNSPPYQMPTTESWKYPGKSCEQLAALFNERKINLSIIAPRKMPVLFKLFMKADGDQPITSKNYAKNIRHLVLLKGYSLKERAPSPNSMAAQMAAPNAAQATVQQQQQQQQNQAGQQQQGQGMPMDTTPAQQQQQQQQQQQQQQQQGNPQQQVMNMNTMQQQQPGPNPPAGLLNPQQQQQLLQQQQQNQFVSNQMQNQNFQQNVGPGQNRWMYPNQPGQARPPFMQGAGNVGGVGQGGGMQQNPNSALISRINAPPPNQTVTSLQQQQQQQAQQQQQQQQQAQQQQQQRMQMLSQQQMLNHQQLQQQQQLAQQQQQQQQGQQQQQVNPNAGNNMMPASNAGNMSNPQQQQQQVGQQSNPQQQGNPQQQQQGNSQQEQASLREKIWTGVLEWSEKPKSDQQKIPHTLQCTVCTNIKDGEPEIKAENWPPKLLMQLMPKHLVGNIGGQFLKDSKMVVFRPTPGEALDSLAKMMTSGYAGCVHFSSIPNSPACDLKVLILLYTPDRNAFLGFIPNNQAMFVERLRKVIQQKQHGNMQQQQQQQQMMQQQGKSPMELQQQQQQQQQQQQMQQDNSQQQHYNQFQLNMQMGGGGPGGGPGPGPGGMPMQQNQMQMNMMQQQRMPLGVGVGVGVGPGGVPNPNLQQQLQQVAPNVAAMQQQQAQQQQQRMVRPMMSNNNPGLRQLLQHQTTPGNQFRPQMGGQNPNQMGAGGPMVGNRNFDDGNYEFM.
CBMOAB-51102FYA	Monoclonal	Rhesus (Macaca mulatta)	WB, ELISA	MO51102FYA		100 µg	Inquiry
Protein Refseq: The length of the protein is 796 amino acids long. The sequence is show below: MVPGSEGPARAGGLVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQPPTDVSQDPRHMVLVRGLVLPVGGGSAPGPLQPKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPGNLSAAQVAAQNAVEAAKNQKAGLGPRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPGAPKPPPASQPSLVSTVAPGSGLAPTAQPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQQRGMGGQQAPPGLGPILEDQARPSQNLLQLRPPQSQPQGTVGASGATGQPQPQGTAQPPPGAPQGPPGTAYGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPPAQSDAAERGSPRPGPPAGPAAQRNPRGHLCPCLERRAVSPGGGEARGKTKERRRGPRVSRKVGASLFLCGSEEHAYVFNMQTNRVCVERIQPETPL.
CBMOAB-86447FYA	Monoclonal	Zebrafish (Danio rerio)	WB, ELISA	MO86447FYA		100 µg	Inquiry
Protein Refseq: The length of the protein is 701 amino acids long. The sequence is show below: MDPTSKSGLNQVSDVVFVIEGTANLGPYFESLRNNYILPTIEYFNGGPPAETDFGGDYGGTQYGLVVFNTVDCAPESYVQCHAPTSSAFEFVSWIDSIQFMGGGAESCSLIAEGLSVALQLFDDFKKMREQIGQTHKVCVLLCNSPPYLLPAVESVSYTGCTADSLVQIIRDRGIHFSVIAPRKLPALRSLFERASSVAGLPESSHPDYSQDPFHMVLVRGIALPVAPGGGSVPVSLKPVLPPQSLPVNQNPLVPVASTAPQMNTFQNVSLHAAHDAAQKALEEAANQQQKNRFGQISTPPFSQSPVLPPGGKPSLSTVTTVSPPVLTQQQVPPQQPQQQTQVPQPGLPAVNQQPPQASQQQPNNQQTPPPTQPGMAAVPAPPPGAQQGVANKVVAWSGVLEWQEKPKASSMDSNTKLTRSLPCQVQVSQGENLNTDQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFLFTNKDMESLKGLFRIMTSGFAGCVHFPHSAPCEVRVLMLLYSSRKRIFMGLIPNDQSGFVNGIRQVITNHKQVQQQRSMQPGQVQPNQNFLNRPPGPIPVTHGNVPQQLRAAATGNQQAPVTGAPPNQVQGQAQAPGGGMLRLQNPGANPQLRSLLLSQQPQGGMQGMQGMHPIQQMVHAAPGGGAQMQPQWRQPHQGPLMVPTGPRGPVTQNPGMPSVSSVMEDEILMDLI.
MO-AB-58936W	Monoclonal	Marmoset	WB, ELISA	MO58936W		100 µg	Inquiry
Protein Refseq: The length of the protein is 747 amino acids long. The sequence is show below: MVPGSEGPARAGGLVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQPPTDVSQDPRHMVLVRGLVLPVGGGSAPGPLQPKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPGNLSAAQVAAQNAVEAAKNQKAGLGPRFSPITPLQQTAPGVGPPFSQAPAPPLPPGPPGAPKPPPASQPSLVSTVAPGSGLAPTAQPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQQRGMGGQQAPPGLGPILEDQARPSQNLLQLRPPQPQPQGTVGASGTTGQPQPQSTAQPPPGAPQGPPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPPAQSWPAQLPPRAPLPGQILMSGGSRGPVPQPGLQPSVMEDDILMDLI.
MO-AB-15522R	Monoclonal	Cattle (Bos taurus)	WB, ELISA	MO15522R		100 µg	Inquiry
Protein Refseq: The length of the protein is 746 amino acids long. The sequence is show below: MVPGSEGPARAGGLVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTETLVQKIGERGIYFSIVSPRKLPALRLLFEKAAPPAMLEPLQPPADVSQDPRHMVLVRGLVLPVGGGSAPGPLQPKQPVPLPPAAPAGA.
MOFY-0223-FY13	Monoclonal	Arabidopsis	WB, ELISA			100 µg	Inquiry

Specifications

Host species	Mouse (Mus musculus)
Species Reactivity	A. thaliana (Arabidopsis thaliana), Arabidopsis, Cattle (Bos taurus), Fruit fly (Drosophila melanogaster), Marmoset, Rhesus (Macaca mulatta), Zebrafish (Danio rerio)
Clone	MO36353FC
Specificity	This antibody binds to Arabidopsis MED25.
Format	Liquid or Lyophilized
Storage	Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use
Purity	> 90% was determined by SDS-PAGE
Purification	Purified with Protein A or G affinity chromatography
Cellular Localization	Nucleus

Application Information

Application	WB, ELISA
Application Notes	ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

Introduction	This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
Product Overview	Mouse Anti-Arabidopsis MED25 (clone MO36353FC) Antibody (CBMOAB-36353FYC) is a mouse antibody against MED25. It can be used for MED25 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative Names	Mediator Complex Subunit 25; Activator Interaction Domain-Containing Protein 1; Activator-Recruited Cofactor 92 KDa Component; ACID1; ARC92; PTOV2; P78; Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog (S. Cerevisiae)
UniProt ID	Q7XYY2
Protein Refseq	The length of the protein is 836 amino acids long. The sequence is show below: MSSEVKQLIVVAEGTAALGPYWQTIVSDYLEKIIRSFCGSELNGERNPVSTVELSLVIFNSHGSYCACLVQRSGWTRDVDIFLHWLSSIQFGGGGFNEVATAEGLAEALMMFSPPSGQAQPSNDLKRHCILITASNPHILPTPVYRPRLQNVERNENGDAQAESRLSDAETVASYFAKCSVSLSVVCPKQLPTIRALYNAGKPNQQSADLSIDTAKNTFYLVLISENFVEACAALSHSATNLPQTQSPVKVDRATVAPSIPVTGQPPAPVSSANGPIQNRQPVSVGPVPTATVKVEPSTVTSMAPVPSFPHIPAVARPATQAIPSIQTSSASPVSQDMVSNAENAPDIKPVVVSGMTPPLRTGPPGGANVNLLNNLSQVRQVMSSAALAGAASSVGQSAVAMHMSNMISTGMATSLPPSQTVFSTGQQGITSMAGSGALMGSAQTGQSPGPNNAFSPQTTSNVASNLGVSQPMQGMNQGSHSGAMMQGGISMNQNMMSGLGQGNVSSGTGGMMPTPGVGQQAQSGIQQLGGSNSSAPNMQLSQPSSGAMQTSQSKYVKVWEGNLSGQRQGQPVLITRLEGYRSASASDSLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMSQHGFLGQLQDKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIPNQQQQQQQQLHQQQQQQQQIQQQQQQQQHLQQQQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQQQQQQQHQLTQLQHHHQQQQQASPLNQMQQQTSPLNQMQQQTSPLNQMQQQQQPQQMVMGGQAFAQAPGRSQQGGGGGQPNMPGAGFMG.

Relate Reference Data

SDS-PAGE

Figure 1 Mouse Anti-Arabidopsis MED25 Antibody in SDS-PAGE.
SDS-PAGE analysis of CBMOAB-36353FYC in reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

ELISA

Figure 2 Mouse Anti-Arabidopsis MED25 Antibody in ELISA.
ELISA analysis of CBMOAB-36353FYC was performed by coating with Arabidopsis MED25 antigen peptide (SGALMGSAQT).
The abscissa represents the antibody dilution fold (*k).