Rabbit Anti-ATG4 Antibody (MO-DKB-02235W)
Cat: MO-DKB-02235W
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- Product Details
Specifications
| Host species | Rabbit (Oryctolagus cuniculus) |
| Species Reactivity | A. thaliana (Arabidopsis thaliana) |
| Immunogen | ATG4 synthetic peptide derived from Arabidopsis AT3G51820. |
| Format | Liquid or Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | Peptide affinity purified |
Application Information
| Application | WB |
| Application Notes | Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user. |
Target
| Introduction | Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Cleaves the C-terminal amino acid of ATG8 to reveal a C-terminal glycine. ATG8 ubiquitin-like activity requires the exposure of the glycine at the C-terminus for its conjugation to phosphatidylethanolamine (PE) and its insertion to membranes, which is necessary for autophagy. The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy. Moreover not only conjugation, but also subsequent ATG8-PE deconjugation performed also by ATG4 is an important step required to delipidate ATG8 to release the protein from membranes, which facilitates multiple events during macroautophagy, and especially for efficient autophagosome biogenesis, the assembly of ATG9-containing tubulovesicular clusters into phagophores/autophagosomes, and for the disassembly of PAS-associated ATG components. ATG8 delipidation by ATG4 also recycles ATG8-PE generated on inappropriate membranes to maintain a reservoir of unlipidated ATG8 that is required for autophagosome formation at the PAS. |
| Product Overview | This product is a Rabbit antibody against the ATG4. It can be used for ATG4 detection in Western Blot. |
| Alternative Names | Cysteine protease ATG4; EC 3.4.22; Autophagy-related protein 4; ATG4; APG4 AUT2; YNL223W; ATG4, Arabidopsis Chl, CHLG, G4, PDE325, PIGMENT DEFECTIVE 325. |
See other products for " ATG4 "
| CBMOAB-00392CR | Mouse Anti-Yeast ATG4 Antibody (CBMOAB-00392CR) |
For Research Use Only | Not For Clinical Use.
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