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- Product Details
- Relate Reference Data
|Host species||Mouse (Mus musculus)|
|Species Reactivity||C. elegans (Caenorhabditis elegans)|
|Specificity||This antibody binds to C. elegans top-2.|
|Storage||Store at 4°C: short-term (1-2weeks)|
Store at -20°C: long-term and future use
|Purity||> 90% was determined by SDS-PAGE|
|Purification||Purified with Protein A or G affinity chromatography|
|Cellular Localization||Nucleus; Cytoskeleton; Other locations|
|Application Notes||ELISA: 1:1000-1:3000|
Other applications are to be developed. The optimal dilution should be determined by the end user.
|Introduction||Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks (By similarity). Essential during mitosis in the adult germline and during embryogenesis for proper segregation of daughter chromosomes (PubMed:20116245, PubMed:23684975, PubMed:27707787). Required for centromere resolution during mitosis (PubMed:18202360). Required for chromosome segregation in anaphase of meiosis I during spermatogenesis (PubMed:27707787). Promotes cleavage furrow stability during cytokinesis upon the presence of chromatin obstructions (PubMed:23684975). Promotes DNA break formation upon zygotic genome activation in the Z2 and Z3 primordial germ cells in L1 larvae, thereby activating a checkpoint response (PubMed:26073019). Essential for embryogenesis (PubMed:18202360).|
|Product Overview||Mouse Anti-C. elegans top-2 (clone MO59224FYC) Antibody (CBMOAB-59224FYC) is a mouse antibody against top-2. It can be used for top-2 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.|
|Alternative Names||Probable DNA-directed RNA polymerase III subunit RPC6; RNA polymerase III subunit C6; DNA-directed RNA polymerase III subunit F; top-2|
|Protein Refseq||The length of the protein is 1520 amino acids long.|
The sequence is show below: MASIKEELISAAESESQVMQIVQAALEGIDMDALTSMTPGLPQTVRQTAINSLLSSKKLAIQQAPGGVLRLKVNTSEQIAGTEEEQVIYSLIEESKTRGIWIKELREGSGLNQLQLRKTLKSLETKKLIKTIKAVGTTRKCYILFSIEPDMSLTGGTFYSDQQLDSELINTLISVCGSYAASRRKHAIDENPNNIQMQREASYIRPQEIAQFITEKRVLNVPLSLEDLERILEVAVLDGTIERRADGKIRACPPRSSISPLVSVPCAICPVVEDCRPGYVISPQSCDYMKNWLADL.
Figure 1 Endogenous topo-II forms axes distinct from the centromere and the condensin complex. Anti- topo-II antibody probing the whole-worm lysate by Western blotting (WB) is shown.
Figure 2 Endogenous topo-II forms axes distinct from the centromere and the condensin complex. One-cell embryos fixed and stained with anti- topo-II antibody and DAPI, at different mitotic stages. The insets show zoomed-in images of a single prometaphase (Prometa.) chromosome.