Mouse Anti-C. elegans top-2 Antibody (CBMOAB-59224FYC)


Cat: CBMOAB-59224FYC
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityC. elegans (Caenorhabditis elegans)
CloneMO59224FYC
SpecificityThis antibody binds to C. elegans top-2.
FormatLyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationNucleus; Cytoskeleton; Other locations

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionControl of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks (By similarity). Essential during mitosis in the adult germline and during embryogenesis for proper segregation of daughter chromosomes (PubMed:20116245, PubMed:23684975, PubMed:27707787). Required for centromere resolution during mitosis (PubMed:18202360). Required for chromosome segregation in anaphase of meiosis I during spermatogenesis (PubMed:27707787). Promotes cleavage furrow stability during cytokinesis upon the presence of chromatin obstructions (PubMed:23684975). Promotes DNA break formation upon zygotic genome activation in the Z2 and Z3 primordial germ cells in L1 larvae, thereby activating a checkpoint response (PubMed:26073019). Essential for embryogenesis (PubMed:18202360).
Product OverviewMouse Anti-C. elegans top-2 (clone MO59224FYC) Antibody (CBMOAB-59224FYC) is a mouse antibody against top-2. It can be used for top-2 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesProbable DNA-directed RNA polymerase III subunit RPC6; RNA polymerase III subunit C6; DNA-directed RNA polymerase III subunit F; top-2
UniProt IDQ23670
Protein RefseqThe length of the protein is 1520 amino acids long.
The sequence is show below: MASIKEELISAAESESQVMQIVQAALEGIDMDALTSMTPGLPQTVRQTAINSLLSSKKLAIQQAPGGVLRLKVNTSEQIAGTEEEQVIYSLIEESKTRGIWIKELREGSGLNQLQLRKTLKSLETKKLIKTIKAVGTTRKCYILFSIEPDMSLTGGTFYSDQQLDSELINTLISVCGSYAASRRKHAIDENPNNIQMQREASYIRPQEIAQFITEKRVLNVPLSLEDLERILEVAVLDGTIERRADGKIRACPPRSSISPLVSVPCAICPVVEDCRPGYVISPQSCDYMKNWLADL.

WB

Figure 1 Endogenous topo-II forms axes distinct from the centromere and the condensin complex. Anti- topo-II antibody probing the whole-worm lysate by Western blotting (WB) is shown.

IF

Figure 2 Endogenous topo-II forms axes distinct from the centromere and the condensin complex. One-cell embryos fixed and stained with anti- topo-II antibody and DAPI, at different mitotic stages. The insets show zoomed-in images of a single prometaphase (Prometa.) chromosome.

For Research Use Only | Not For Clinical Use.

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