Mouse Anti-C. elegans top-2 Antibody (CBMOAB-59224FYC)
Cat: CBMOAB-59224FYC
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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- Specifications
- Application Information
- Target
- Relate Reference Data
Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | C. elegans (Caenorhabditis elegans) |
| Clone | MO59224FYC |
| Specificity | This antibody binds to C. elegans top-2. |
| Format | Liquid or Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
| Cellular Localization | Nucleus; Cytoskeleton; Other locations |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Introduction | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks (By similarity). Essential during mitosis in the adult germline and during embryogenesis for proper segregation of daughter chromosomes (PubMed:20116245, PubMed:23684975, PubMed:27707787). Required for centromere resolution during mitosis (PubMed:18202360). Required for chromosome segregation in anaphase of meiosis I during spermatogenesis (PubMed:27707787). Promotes cleavage furrow stability during cytokinesis upon the presence of chromatin obstructions (PubMed:23684975). Promotes DNA break formation upon zygotic genome activation in the Z2 and Z3 primordial germ cells in L1 larvae, thereby activating a checkpoint response (PubMed:26073019). Essential for embryogenesis (PubMed:18202360). |
| Product Overview | Mouse Anti-C. elegans top-2 (clone MO59224FYC) Antibody (CBMOAB-59224FYC) is a mouse antibody against top-2. It can be used for top-2 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | Probable DNA-directed RNA polymerase III subunit RPC6; RNA polymerase III subunit C6; DNA-directed RNA polymerase III subunit F; top-2 |
| UniProt ID | Q23670 |
| Protein Refseq | The length of the protein is 1520 amino acids long. The sequence is show below: MASIKEELISAAESESQVMQIVQAALEGIDMDALTSMTPGLPQTVRQTAINSLLSSKKLAIQQAPGGVLRLKVNTSEQIAGTEEEQVIYSLIEESKTRGIWIKELREGSGLNQLQLRKTLKSLETKKLIKTIKAVGTTRKCYILFSIEPDMSLTGGTFYSDQQLDSELINTLISVCGSYAASRRKHAIDENPNNIQMQREASYIRPQEIAQFITEKRVLNVPLSLEDLERILEVAVLDGTIERRADGKIRACPPRSSISPLVSVPCAICPVVEDCRPGYVISPQSCDYMKNWLADL. |
Relate Reference Data
WB
Figure 1 Endogenous topo-II forms axes distinct from the centromere and the condensin complex. Anti- topo-II antibody probing the whole-worm lysate by Western blotting (WB) is shown.
IF
Figure 2 Endogenous topo-II forms axes distinct from the centromere and the condensin complex. One-cell embryos fixed and stained with anti- topo-II antibody and DAPI, at different mitotic stages. The insets show zoomed-in images of a single prometaphase (Prometa.) chromosome.
For Research Use Only | Not For Clinical Use.
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