Mouse Anti-C. elegans zfp-1 Antibody (CBMOAB-59600FYC)
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Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | C. elegans (Caenorhabditis elegans) |
| Clone | MO59600FYC |
| Specificity | This antibody binds to C. elegans zfp-1. |
| Format | Liquid or Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
| Cellular Localization | Nucleus; Other locations |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Introduction | Recruits the histone methyltransferase dot-1.1 to chromatin to methylate 'Lys-79' of histone H3 and activate transcription (PubMed:23806335). Recognizes and binds histone H3 methylated at 'Lys-4' (H3K4me) at the promoters of target genes (PubMed:23263989). During stress, the zfp-1-dot-1.1 complex also plays a role in the deubiquitination of histone H2B sites, which negatively modulates the RNA polymerase II-induced transcription of highly expressed genes (PubMed:23806335). In response to stress, binds to the pdk-1 promoter to negatively regulate pdk-1 expression, which negatively modulates daf-16/FOXO-mediated gene expression (PubMed:21980302). Thus, most likely via this mechanism, in response to stress, it confers a protective role against neuronal necrosis (PubMed:25422944). Plays a role in Insulin/IGF-1-like signaling (IIS)- and diet restriction-mediated lifespan extension by controlling daf-16/FOXO and pha-4/FOXA recruitment to target promoters (PubMed:27039057). May negatively regulate the expression of genes required for vulval development (PubMed:16507136, PubMed:16710447). May play a role in axon guidance in D-type motor neurons (PubMed:24066155). May suppress sensitivity to RNAi (PubMed:16507136). |
| Product Overview | Mouse Anti-C. elegans zfp-1 (clone MO59600FYC) Antibody (CBMOAB-59600FYC) is a mouse antibody against zfp-1. It can be used for zfp-1 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | Uncharacterized protein F54F2.2, isoform a; zfp-1 |
| UniProt ID | P34447 |
| Protein Refseq | The length of the protein is 867 amino acids long. The sequence is show below: MKEMVGGCCVCADENGWTDNPLIYCDGENCEVAVHQGCYGIQEVPEGEWFCAKCTKASAMMPGSINEATFCCQLCPFDYGALKKTDRNGWAHVICALYIPEVRFGNVHSMEPVILNDVPTDKFNKLCYICNEERPNDAKKGACMSCNKSTCKRSFHVTCAQRKGLLCEEGAISRNVKYCGYCENHLKKAINDPAIKVIPACPPVQRLSKEQDKKKTVLLTSLPLPPPAPRLHMLADPLPIKSNKVNNVLGLGSAINAVSLEERPASGSTVNSGIFVPPPTAFSPPLTTSSRSSVAQDPSPPLTINKNSLSSSGPLIPSTAHLSATTASATPIMANGSTLPPSSETTVGTHCLQQLQIQSAAAAAITQNQIGPSELNGYPAASQLSSFMHEIPARNTTSVASLLPPGAAEYHLNGSGDEEKTVKAVLTAPLTKAKRIRDSKNDMMDKTHKRPRANARPPAVLGSMSSGSSGGTVGKSPSMQRLQNLVAPIVSETVTDFQRDRVADRTAAERRAAAAQSQPSTSTNGGPNVTIPAVVEVHTNSTNSTNHQNNGLTQNAPASTSMQAGTSSNDGVISQNGTSSTSQSNRLNLPSFMEQLLERQWDQGSSLLMANAHFDVAQLLSCLFQLKSENFRLEENLSGLRKRRDHLFALNSRLAEVNTLDVSKRQRSDGLLLQQQIAHHLDPTSIVPKAEVHKQEPLSAPTSVPLPANHSSSLFEDIKAPKATYSRKTPSNIPLTVPLSTAATALTTTTAASSGAPVNSNIQNHRATPSTAGAPMAATPIMTAVTSANELAALSPERAQALLNMYRMPLDANVAAQLSMITNFPGQVNPSLFSRLLAVNMMNGGLQPNGQPLSALPPPTSATPNGK. |
WB
Figure 1 ZFP-1(AF10) and DOT-1.1 Interact and Colocalize to Promoters of Target Genes. The truncated ZFP-1 protein similar to that present in the zfp-1(ok554) mutant strain does not interact with DOT-1.1. Top, a schematic of the two FLAG-tagged WT ZFP-1 isoforms and a truncated ZFP-1 protein missing the C-terminal portion, which is identical to that present in the zfp-1(ok554) mutant. Bottom, western blots with an antibody against DOT-1.1 and an antibody against ZFP-1 of total lysates (left) and proteins immunoprecipitating with an antibody against FLAG (right) in indicated transgenic strains or WT nontransgenic worms, rightmost lanes.
IF
Figure 2 The long isoform of ZFP-1 is specifically expressed in the germ line. Immunostaining of dissected gonads from adult worms by use of an anti-ZFP-1 N terminus-specific antibody (pink) for wild-type worms compared to zfp-1(ok554) worms and worms with knockdown of zfp-1 in the zfp-1(ok554) mutant background by RNAi. The left and middle panels also show parts of the dissected intestine (bottom right insets in the middle panels). DAPI-stained DNA is shown in blue, oocyte nuclei are indicated with arrows, and intestinal nuclei are indicated with arrowheads. Enlargements of the indicated regions of the right panels are shown on the far right.
WB
Figure 3 Knockdown of ZFP-1 by RNAi in wild-type worms leads to protein depletion. A Western blot probed with anti-ZFP-1 C terminus-specific antibody shows the depletion of all ZFP-1 bands upon RNAi. A Ponceau-stained membrane is shown as a loading control.
IF
Figure 4 ZFP-1::GFP localizes to chromosomes in maturing oocytes and is widely expressed at all developmental stages.