Mouse Anti-GLU1 Antibody (CBMOAB-34127FYC)

Sub Cat	Clonality	Species Reactivity	Application	Clone	Conjugate	Size
CBMOAB-34127FYC	Monoclonal	A. thaliana (Arabidopsis thaliana), Arabidopsis (Arabidopsis lyrata), Arabidopsis, Rice, Maize (Zea mays), Rice (Oryza)	WB, ELISA	MO34127FC		100 µg	Inquiry
Protein Refseq: The length of the protein is 1622 amino acids long. The sequence is show below: MALQSPGATGASSSVSRLLSSAKLSSTKTIFSVDFVRSYCISKGTKRRNELSGFRGYSPLLKSSLRSPFSVKAILNSDRAAGDASSSFSDLKPQVAYLEDIISERGACGVGFIANLENKATHKIVNDALIALGCMEHRGGCGSDNTSGDGSGLMTSIPWDLFNEWAEKQGIASFDRTHTGVGMLFLPRDDNIRKEAKKVITSIFEKEGLEVLGWRDVPVEASIVGHNAKQTMPNTEQVFVRIVKDDKVDDVERELYICRKLIERAVASESWASELYFSSLSNQTIVYKGMLRSEVLGLFYPDLQNDLYKSPFAIYHRRFSTNTSPRWHLAQPMRFLGHNGEINTIQGNLNWMTSREASLRSPVWHGRENDIRPISNPKASDSANLDSAAELLIRSGRTPEESLMILVPEAYKNHPTLMIKYPEAVDFYDYYKGQMEPWDGPALVLFSDGKTVGACLDRNGLRPARYWRTSDNVVYVASEVGVLPMDESKVTMKGRLGPGMMISVDLENGQVYENTEVKKRVASYNPYGKWVSENLRNLKPSNYLSSAILETDETLRRQQAFGYSSEDVQMVIESMAAQGKEPTFCMGDDTPVAVLSQKPHMLYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILEVGPQNVSQVVLSGPVLNERELEGLLGDPLLKSQILPTFFDIRRGIEGSLKKGLLKLCEAADEAVRNGSQVLVLSDRSDNPEPTRPAIPMLLAVGAVHQHLIQNGLRMSASIIADTAQCFSTHHFACLIGYGASAICPHLALETCRQWRLSNKTVNMMRNGKMPTVTMEQAQKNYRKAVNTGLLKVLSKMGISLFSSYCGAQIFEIYGLGNEVVEFSFRGSASQIGGLTLDELARETLTFWVRAFSEDTAKRLENFGFIQFRPGGEYHGNNPEMSKLLHKAVREKSETAYAVYQQHLANRPITVFRDLLEFKSDRNPIPVGKVEPASSIVERFCTGGMSLGAISRETHETIAIAMNRLGGKSNSGEGGEDPIRWKPLTDVVDGYSSTLPHLKGLRNGDTATSAIKQVASGRFGVTPTFLVNADQLEIKVAQGAKPGEGGQLPGKKVSAYIARLRNSKPGVPLISPPPHHDIYSIEDLAQLIFDLHQVNPKAKVSVKLVSETGIGTVASGVAKANADIIQISGYDGGTGASPISSIKHAGGPWELGLAETQKTLIGNGLRERVIIRVDGGFKSGVDVLIAAAMGADEYGFGTLAMIATGCIMARICHTNNCPVGVASQREELRARFPGLPGDLVNFFLYIAEEVRGILAQLGYEKLDDIIGRTDLLKARDISLVKTHLDLSYLLSSVGLPKRSSTSIRKQEVHSNGPVLDDTLLQDPEIMDAIENEKTVHKTMSIYNVDRSVCGRIAGVIAKKYGDTGFAGQLNLTFTGSAGQSFACFLTPGMNIRLVGEANDYVGKGMAGGEVVILPVESTGFRPEDATIVGNTCLYGATGGLLFVRGKAGERFAVRNSLAQAVVEGTGDHCCEYMTGGCVVILGKVGRNVAAGMTGGLAYILDEDNTLLPKMNKEIVKIQRVTSPVGQTQLKSLIQAHVEKTGSSKGAMIVEEWDKYLAMFWQLVPPSEEDTPEANSDHILKTTTGDEEQVSSTLAEK.
CBMOAB-22888FYB	Monoclonal	Rice (Oryza)	WB, ELISA	MO22888FYB		100 µg	Inquiry
Protein Refseq: The length of the protein is 619 amino acids long. The sequence is show below: MSMYGRDPWGGPLEICHDSATDDDRSRNLDLDRGALSRTLDETQQSWLLAGPGDQGRKKKKYVDLGCLVVSRKLFVWTVGVLLAAAVFAGLVAGIAKAIPRHHRPPPPPDDFTVALRKALMFFNAQKSGKLPKNNNVHWRGNSCMKDGLSDPAVGRSLVGGYYDAGDAVKFNFPAAFSMTLLSWSVIEYSAKYEAVGELGHIRDTIKWGADYFLKTFNSTADTIDRVVMQVGSGATSPGSTQPNDHYCWMRPEDIDYPRPVVECHACSDLAAEMAASLAAASIVFKDNKAYSQKLVHGATTLFKFARQNRGRYSAGGSDAAKFYNSTSYWDEFVWGGSWMYLATGNSSYLQLATHPKLAKHAGAYWGGPDYGVFSWDNKLTGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIIMCSYLPIFKSFNRTKGGLIQLNHGRPQPLQYVVNAAFLASLYGDYLEAADTPGWYCGPHFYPIETLRNFARTQIEYILGKNPLKMSYVVGYGNRYPKRVHHRGASIPKNGVHYGCKGGWKWRETKKPNPNIIVGAMVAGPDRHDGFKDVRKNYNYTEATLAGNAGLVAALVALSGEGHGVDKNTMFSAVPPMFPSPPPPPAPWKP.
MO-AB-48223W	Monoclonal	Maize (Zea mays)	WB, ELISA	MO48223W		100 µg	Inquiry
Protein Refseq: The length of the protein is566 amino acids long. The sequence is show below: MAPLLAAAMNHAAAHPGLRSHLVGPNNESFSRHHLPSSSPQSSKRRCNLSFTTRSARVGSQNGVQMLSPSEIPQRDWFPSDFTFGAATSAYQIEGAWNEDGKGESNWDHFCHNHPERILDGSNSDIGANSYHMYKTDVRLLKEMGMDAYRFSISWPRILPKGTKEGGINPDGIKYYRNLINLLLENGIEPYVTIFHWDVPQALEEKYGGFLDKSHKSIVEDYTYFAKVCFDNFGDKVKNWLTFNEPQTFTSFSYGTGVFAPGRCSPGLDCAYPTGNSLVEPYTAGHNILLAHAEAVDLYNKHYKRDDTRIGLAFDVMGRVPYGTSFLDKQAEERSWDINLGWFLEPVVRGDYPFSMRSLARERLPFFKDEQKEKLAGSYNMLGLNYYTSRFSKNIDISPNYSPVLNTDDAYASQEVNGPDGKPIGPPMGNPWIYMYPEGLKDLLMIMKNKYGNPPIYITENGIGDVDTKETPLPMEAALNDYKRLDYIQRHIATLKESIDLGSNVQGYFAWSLLDNFEWFAGFTERYGIVYVDRNNNCTRYMKESAKWLKEFNTAKKPSKKILTPA.
MO-AB-00533H	Monoclonal	Arabidopsis (Arabidopsis lyrata)	WB, ELISA	MO00533C		100 µg	Inquiry
Protein Refseq: The length of the protein is 1606 amino acids long. The sequence is show below: MAMKSLSPIPKLLSTTPSSVLSSDKNFFFVDFVGLYCKSKRTRRRLRGDSSSGSSSSSLSRLSSVRAVIDLERLNGVSHKDLSSTSLLKPQVANLEDILSERGACGVGFIANLDNIPSHGVVKDALIALGCMEHRGGCGADNDSGDGSGLMSSIPWDFFNVWAKEQGLSPFDKLHTGVGMIFLPQEDTFMQEAKQVIENIFEKEGLQVLGWREVPVNVPIVGKNARETMPNIQQVFVKIAKEDSTDDIERELYICRKLIERAVATESWGTELYFCSLSNQTIVYKGMLRSEALGLFYLDLQNELYESPFAIYHRRYSTNTSPRWPLAQPMRFLGHNGEINTIQGNLNWMQSREASLKSSVWNGRENEIRPFGNPRGSDSANLDSAAEILIRSGRTPEEALMILVPEAYKNHPTLSVKYPEVIDFYDYYKGQMEAWDGPALLLFSDGKTVGACLDRNGLRPARYWRTSDNVVYVASEVGVVPVDEAKVTMKGRLGPGMMIAVDLVNGQVYENTEVKKRISSFNPYGKWIKENSRFLKPVNFKSSTVMENEEILRSQQAFGYSSEDVQMVIESMASQGKEPTFCMGDDIPLAGLSQRPHMLYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILELGPENASQVILSNPVLNEGTLEELMKDTYLKPKVLSTYFDIRKGVEGSLQKALYSLCEAADDAVRSGSQLLVLSDRSDSLEPTRPAIPIMLAVGAVHQHLIQNVSFVCSEFSKFVPANAVCPYLALETCRQWRLSNKTVAFMRNGKIPTVTIEQAQKNYTKAVNAGLLKILSKMGISLLSSYCGAQIFEIYGLGKEVVDLAFTGSVSKISGLTFDELARETLSFWVKAFSEDTTKRLENFGFIQFRPGGEYHSNNPEMSKLLHKAVREKSETAYAVYQQHLSNRPVNVLRDLLEFKSDRAPIPVGKVEPAVSIVQRFCTGGMSLGAISRETHEAIAIAMNRIGGKSNSGEGGEDPIRWKPLTDVVDGYSSTLPHLKGLQNGDIATSAIKQVASGRFGVTPTFLVNADQLEIKVAQGAKPGEGGQLPGKKVSAYIARLRSSKPGVPLISPPPHHDIYSIEDLAQLIFDLHQINPNAKVSVKLVAEAGIGTVASGVAKGNADIIQISGHDGGTGASPISSIKHAGGPWELGLTETHQTLIENGLRERVILRVDGGLKSGVDVLMAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQREELRARFPGVPGDLVNYFLYVAEEVRGILAQLGYSKLDDIIGRTELLKPRDISLVKTQHLDLSYLLSSVGTPSLSSTEIRKQEVHTNGPVLDDDILADPLVIDAIENEKVVDKTVKICNVDRAVCGRVAGVIAKKYGDTGFAGQVNLTFLGSAGQSFGCFLIPGMNIRLIGESNDYVGKGMAGGEIVVTPVDTIGFVPEEATIVGNTCLYGATGGQIFARGKAGERFAVRNSLAEAVVEGTGDHCCEYMTGGCVVVLGKVGRNVAAGMTGGLAYLLDEDDTLLPKINREIVKMQRVTAPAGELQLKSLIEAHVEKTGSSKGATILNEWDKYLPLFWQLVPPSEEDTPEASAAYVRTATGEVTFQSA.
MO-DKB-00108W	Polyclonal	A. thaliana (Arabidopsis thaliana)	ELISA, WB			100 µg	Inquiry
MO-DKB-01796W	Polyclonal	A. thaliana (Arabidopsis thaliana)	WB			100 µg	Inquiry
MOFAB-276W		Arabidopsis, Rice	WB			100 µg	Inquiry

Specifications

Host species	Mouse (Mus musculus)
Species Reactivity	A. thaliana (Arabidopsis thaliana), Arabidopsis (Arabidopsis lyrata), Arabidopsis, Rice, Maize (Zea mays), Rice (Oryza)
Clone	MO34127FC
Specificity	This antibody binds to Arabidopsis GLU1.
Format	Liquid or Lyophilized
Storage	Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use
Purity	> 90% was determined by SDS-PAGE
Purification	Purified with Protein A or G affinity chromatography
Cellular Localization	Chloroplast; Mitochondrion; Plasma Membrane; Extracellular region or secreted

Application Information

Application	WB, ELISA
Application Notes	ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

Introduction	GNC and CGA1 were found to alter the expression of chloroplast-localized glutamate synthase (GLU1/Fd-GOGAT), a major factor controlling nitrogen assimilation in green tissues.
Product Overview	Mouse Anti-Arabidopsis GLU1 (clone MO34127FC) Antibody (CBMOAB-34127FYC) is a mouse antibody against GLU1. It can be used for GLU1 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative Names	Ferredoxin-dependent glutamate synthase 1, chloroplastic/mitochondrial; EC 1.4.7.1; Fd-GOGAT 1; GLU1; GLS1; At5g04140
UniProt ID	Q9ZNZ7
Protein Refseq	The length of the protein is 1622 amino acids long. The sequence is show below: MALQSPGATGASSSVSRLLSSAKLSSTKTIFSVDFVRSYCISKGTKRRNELSGFRGYSPLLKSSLRSPFSVKAILNSDRAAGDASSSFSDLKPQVAYLEDIISERGACGVGFIANLENKATHKIVNDALIALGCMEHRGGCGSDNTSGDGSGLMTSIPWDLFNEWAEKQGIASFDRTHTGVGMLFLPRDDNIRKEAKKVITSIFEKEGLEVLGWRDVPVEASIVGHNAKQTMPNTEQVFVRIVKDDKVDDVERELYICRKLIERAVASESWASELYFSSLSNQTIVYKGMLRSEVLGLFYPDLQNDLYKSPFAIYHRRFSTNTSPRWHLAQPMRFLGHNGEINTIQGNLNWMTSREASLRSPVWHGRENDIRPISNPKASDSANLDSAAELLIRSGRTPEESLMILVPEAYKNHPTLMIKYPEAVDFYDYYKGQMEPWDGPALVLFSDGKTVGACLDRNGLRPARYWRTSDNVVYVASEVGVLPMDESKVTMKGRLGPGMMISVDLENGQVYENTEVKKRVASYNPYGKWVSENLRNLKPSNYLSSAILETDETLRRQQAFGYSSEDVQMVIESMAAQGKEPTFCMGDDTPVAVLSQKPHMLYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILEVGPQNVSQVVLSGPVLNERELEGLLGDPLLKSQILPTFFDIRRGIEGSLKKGLLKLCEAADEAVRNGSQVLVLSDRSDNPEPTRPAIPMLLAVGAVHQHLIQNGLRMSASIIADTAQCFSTHHFACLIGYGASAICPHLALETCRQWRLSNKTVNMMRNGKMPTVTMEQAQKNYRKAVNTGLLKVLSKMGISLFSSYCGAQIFEIYGLGNEVVEFSFRGSASQIGGLTLDELARETLTFWVRAFSEDTAKRLENFGFIQFRPGGEYHGNNPEMSKLLHKAVREKSETAYAVYQQHLANRPITVFRDLLEFKSDRNPIPVGKVEPASSIVERFCTGGMSLGAISRETHETIAIAMNRLGGKSNSGEGGEDPIRWKPLTDVVDGYSSTLPHLKGLRNGDTATSAIKQVASGRFGVTPTFLVNADQLEIKVAQGAKPGEGGQLPGKKVSAYIARLRNSKPGVPLISPPPHHDIYSIEDLAQLIFDLHQVNPKAKVSVKLVSETGIGTVASGVAKANADIIQISGYDGGTGASPISSIKHAGGPWELGLAETQKTLIGNGLRERVIIRVDGGFKSGVDVLIAAAMGADEYGFGTLAMIATGCIMARICHTNNCPVGVASQREELRARFPGLPGDLVNFFLYIAEEVRGILAQLGYEKLDDIIGRTDLLKARDISLVKTHLDLSYLLSSVGLPKRSSTSIRKQEVHSNGPVLDDTLLQDPEIMDAIENEKTVHKTMSIYNVDRSVCGRIAGVIAKKYGDTGFAGQLNLTFTGSAGQSFACFLTPGMNIRLVGEANDYVGKGMAGGEVVILPVESTGFRPEDATIVGNTCLYGATGGLLFVRGKAGERFAVRNSLAQAVVEGTGDHCCEYMTGGCVVILGKVGRNVAAGMTGGLAYILDEDNTLLPKMNKEIVKIQRVTSPVGQTQLKSLIQAHVEKTGSSKGAMIVEEWDKYLAMFWQLVPPSEEDTPEANSDHILKTTTGDEEQVSSTLAEK.

Reference

1. Hudson, D., Guevara, D., Yaish, M. W., Hannam, C., Long, N., Clarke, J. D., ... & Rothstein, S. J. (2011). GNC and CGA1 modulate chlorophyll biosynthesis and glutamate synthase (GLU1/Fd-GOGAT) expression in Arabidopsis. PloS one, 6(11), e26765.
2. Badia, M. B., Maurino, V. G., Pavlovic, T., Arias, C. L., Pagani, M. A., Andreo, C. S., ... & Gerrard Wheeler, M. C. (2020). Loss of function of Arabidopsis NADP-malic enzyme 1 results in enhanced tolerance to aluminum stress. The Plant Journal, 101(3), 653-665.
3. Gaufichon, L., Rothstein, S. J., & Suzuki, A. (2016). Asparagine metabolic pathways in Arabidopsis. Plant and Cell Physiology, 57(4), 675-689.

Relate Reference Data

Figure 1 Chromatin immunnoprecipitation (ChIP) of myc-tagged GNC and CGA1 protein indicates interaction with the GLU1 promoter. PCR of promoter regions containing GATA sites from suspected candidate genes following precipation with c-myc antibody using extracts from wild type plants and transgenics lines expressing myc-tagged GNC or CGA1.
Reference: Hudson, D., Guevara, D., Yaish, M. W., Hannam, C., Long, N., Clarke, J. D., ... & Rothstein, S. J. (2011). GNC and CGA1 modulate chlorophyll biosynthesis and glutamate synthase (GLU1/Fd-GOGAT) expression in Arabidopsis. PloS one, 6(11), e26765.