Anti-H3K4me3 Antibody (Cat MO-MMB-0696), Rabbit IgG


Cat: MO-MMB-0696
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Lot Number

  • Product List
  • Specifications
  • Application Information
  • Target
Sub Cat Clonality Species Reactivity Application Clone Conjugate Size  
MO-MMB-0696 Polyclonal Budding Yeast, Human (Homo sapiens), Mouse (Mus musculus) ChIP-qPCR, ChIP-chip, ChIP-Seq, Native ChIP, WB, IF, FC, IHC(P), ELISA, PLA 100 µg
MO-MMB-0697 Polyclonal Budding Yeast, Human (Homo sapiens) ChIP-qPCR, ChIP-Seq, Native ChIP, IF, WB 100 µg

Specifications

Host speciesRabbit (Oryctolagus cuniculus)
Species ReactivityBudding Yeast, Human (Homo sapiens), Mouse (Mus musculus)
ImmunogenThis histone H3 trimethyl Lys4 antibody is raised against peptides including the trimethyl lysine 4 of histone H3.
FormatLiquid or Lyophilized
Buffer30% Glycerol
Regulatory StatusFor Research Use Only
ShippingGel Pack
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use

Application Information

ApplicationChIP-qPCR, ChIP-chip, ChIP-Seq, Native ChIP, WB, IF, FC, IHC(P), ELISA, PLA
Application NotesChIP: 3-5 µL per ChIP
ChIP-Seq: 3 µL each
IF: 1:500-1:1000
WB: 1:500-1:2000
The optimal dilution should be determined by the end user.

Target

IntroductionHistone H3 is one of the core components of nucleosomes. The nucleosome is the smallest subunit of chromatin and consists of 147 DNA base pairs wrapped around the core histone octamer (histone H2A, histone H2B, histone H3, and histone H4). Histone H1 is a linking histone protein that exists at the interface between the nucleosome core and the DNA entry/exit point. Histone H1 is responsible for building higher-order chromatin structures. Chromatin undergoes a variety of chemical modifications, including post-translational modifications of histones and methylation of cytosine residues in DNA. The reported histone modifications include acetylation, methylation, phosphorylation, ubiquitination, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Alternative NamesH3K4me3; Histone H3K4me3
For Research Use Only | Not For Clinical Use.
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