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- Product Details
|Host species||Mouse (Mus musculus)|
|Specificity||This antibody binds to Yeast APC5.|
|Storage||Store at 4°C: short-term (1-2weeks)|
Store at -20°C: long-term and future use
|Purity||> 90% was determined by SDS-PAGE|
|Purification||Purified with Protein A or G affinity chromatography|
|Application Notes||ELISA: 1:1000-1:3000|
Other applications are to be developed. The optimal dilution should be determined by the end user.
|Introduction||Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication.|
|Product Overview||Mouse Anti-Yeast APC5 (clone MO00015CR) Antibody (CBMOAB-00015CR) is a mouse antibody against APC5. It can be used for APC5 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.|
|Alternative Names||Anaphase-promoting complex subunit 5; APC5; RMC1; YOR249C|
|Protein Refseq||The length of the protein is 685 amino acids long. The sequence is show below: MSKYGPLGITNFITPYDLCILILIHAHCSQDNGISVPTAVFLRLISPTRPSLEWNPLLKDNSNLRSSSIVPPPVLPILDNIIRILLDDKDGNKIALTLMGYLEAINGLDSINRLMMDLEKNCLVNNYRSMKMRTTSTRRQMTRASFLGTFLSTCIRKYQIGDFEMRETIWINLQNFKTVFKHTPLWLRFKDNVHIQKVKNCLLANDEISVEDQQMVEFFQHFNNGNDADSKTMNEENYGTLISIQHLQSIVNRQIVNWLDNTEFNLMGQEETSSTYEEQSGLVFDLLDTLSLNDATKFPLIFILKYLEAIKENSYQTALDSLHNYFDYKSTGNSQNYFHISLLSLATFHSSFNECDAAINSFEEATRIARENKDMETLNLIMIWIINFIEVHPEYANRFYITVEQIIKYLKNSSDVEDANIFSNAYKFETLLSMVKESKTAEVSSSLLKFMAITLQNVPSQNFDLFQSLVSYEVKFWKELGYESISDVYEKFLSKTSSSSLRNYDSSIINQDIKVAFKALEEDDFLKVKQYLLKSESLELDYDQKINLKYLRVKYLVKIGDYDLSMRLINQYVKECCEEVADSNWRFKFEIESINVLLLSDVGIRSLPKIIKLIDEYKEIGNPLRCVILLL.|
See other products for " APC5 "
|CBMOAB-2258FYC||Mouse Anti-Arabidopsis APC5 Antibody (CBMOAB-2258FYC)|