Mouse Anti-Yeast HEL2 Antibody (CBMOAB-01657CR)
Cat: CBMOAB-01657CR
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Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | Yeast |
| Clone | MO01657CR |
| Specificity | This antibody binds to Yeast HEL2. |
| Format | Liquid or Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Introduction | Probable ubiquitin-protein ligase involved in the degradation-related ubiquitination of histones. Contributes to the post-translational regulation of histone protein levels by polyubiquitination of excess histones for subsequent degradation. |
| Product Overview | Mouse Anti-Yeast HEL2 (clone MO01657CR) Antibody (CBMOAB-01657CR) is a mouse antibody against HEL2. It can be used for HEL2 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | E3 ubiquitin-protein ligase HEL2; EC 6.3.2; Histone E3 ligase 2; HEL2; YDR266C |
| UniProt ID | Q05580 |
| Protein Refseq | The length of the protein is 639 amino acids long. The sequence is show below: MSESVKENVTPTRNFRRTQGPQNNTKPHNDRKNFRRKQKKNNLSAEPNLTTSSADDTDEENELCVICARKLTYVSLTPCHHKTCHICGFRQRALYNKKSCLICRTENEEVMFTDRIDGDISDKYNFCEKNEKYGINFTSEEVATETLNLLKFFCPLSKDEQVCDFGSFKKYNEHLKSEHNRMICLICATHKHAFPCELEIFTQNQLRNHQTKGNSEGFKGHPMCAFCSGKRFYSDDELYIHMRNQHEKCHICDKMNPASPQYFKDYNQLFDHFKHSHYVCTVQTCLDNKFVVFKDELELQAHILQEHGNILKGKPKFFQSELSTFISAPSRVIRERDDYDLPSISSLPGSSSGSRTDVRSASSPEESRLRLAERAKYYLENSKEDFNKFSSYNEDYSKGRLSAEKLLESYKLLFTKPNADVYLLIHNLAETFPKNSSKYNNLNAIYEQREQTLARQTSLPSLSSDSSLSMSIGRGHWGGTNDGGSAGAALGVRNIKNLPTLKSPSASYDPFATTVKKNTLRPVQNIKRTTPQSVSYRTSTNTVAFSPTYLESKKGSSSTSLNNSKDKLKSLNLPQLPPPKPKVQIPGLNRPQIADPKQWGKKSSTQDTNVHDNLRELNTTSGGNKKKGKQKQLLFHIGV. |
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SDS-PAGE
Figure 1 Mouse Anti-Yeast HEL2 Antibody (CBMOAB-01657CR) in SDS-PAGE.
SDS-PAGE analysis of CBMOAB-01657CR in reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
ELISA
Figure 2 Mouse Anti-Yeast HEL2 Antibody (CBMOAB-01657CR) in ELISA.
ELISA analysis of CBMOAB-01657CR was performed by coating with Yeast HEL2 antigen peptide (RSASSPEESR).
The abscissa represents the antibody dilution fold (*k).
For Research Use Only | Not For Clinical Use.
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