Mouse Anti-Yeast MCM5 Antibody (CBMOAB-02182CR)


Cat: CBMOAB-02182CR
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityYeast
CloneMO02182CR
SpecificityThis antibody binds to Yeast MCM5.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationNucleus; Other locations

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionThe protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Product OverviewMouse Anti-Yeast MCM5 (clone MO02182CR) Antibody (CBMOAB-02182CR) is a mouse antibody against MCM5. It can be used for MCM5 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesMinichromosome maintenance protein 5; EC 3.6.4.12; Cell division control protein 46; MCM5; CDC46; YLR274W
UniProt IDP29496
Protein RefseqThe length of the protein is 775 amino acids long. The sequence is show below: MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYSLTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENTSMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLSIMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDPYIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSIYNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQLSRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGTAKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCIDEFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNIDFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMKRYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRITESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKRRLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV.

SDS-PAGE

Figure 1 Mouse Anti-Yeast MCM5 Antibody in SDS-PAGE.
SDS-PAGE analysis of CBMOAB-02182CR in reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 55 kDa and 25 kDa, respectively.

ELISA

Figure 2 Mouse Anti-Yeast MCM5 Antibody in ELISA.
ELISA analysis of CBMOAB-02182CR was performed by coating with Yeast MCM5 antigen peptide (DEFDKMRDED).
The abscissa represents the antibody dilution fold (*k).

For Research Use Only | Not For Clinical Use.
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