C43(DE3) pLysS competent cells (MOFY-0822-FY504)
Cat: MOFY-0822-FY504
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Specifications
| Description | C43(DE3) pLysScompetent cells Both the OverExpress C43 (DE3) and OverExpress C41 (DE3) strains originated from BL21 (DE3), and their advantages are that they can express toxic or hydrophobic proteins with high efficiency. |
| Gene Types | F-ompT hsdSB (rB- mB-) gal dcm (DE3)pLysS CamR |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 1×108 cfu/μg DNA |
| Note | 1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating. 3. During induction, IPTG concentration is optional (0.1-2 mM can be used). 4. In order to obtain the required amount of protein, the induction time, temperature and IPTG concentration need to be optimized by the experimenter. 5. The C43(DE3)pLysS strain carries the pLysS plasmid. Except for the recovery medium without antibiotics, the other medium and medium should contain 34 µg/ml chloramphenicol to prevent the loss of the plasmid. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
For Research Use Only | Not For Clinical Use.
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