Drosophila gene expression vector pUAST plasmid


Cat: MOFY-0123-FY11
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Specifications

Species SourceD. melanogaster
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CompositionP-element 3' end; 5×UAS/mini_Hsp70; Kozak; ORF; SV40 terminator; mini-white; P-element 5' end; pUC ori
BufferRefer to COA

Plasmid Information

Product OverviewOur pUAST vector system is a good and efficient transgenic Drosophila production and gene expression control system. The system is derived from the commonly used Drosophila P-element transposon, combined with the strongly inducible promoter Gal4 to regulate transgene expression.
The complete pUAST system consists of two vectors, one vector is called pUAST plasmid, which contains two P element terminal repeats, including the region or gene to be transposed; the other vector is called helper plasmid or transposase plasmid, encoding P transposition Seat enzyme.
When pUAST and transposase plasmids are co-transfected into target cells, the transposase produced by the helper plasmid recognizes the two P-element terminal repeats on the pUAST plasmid, and then inserts the region to be transposed and the two P-element terminal repeats into in the host genome.
Regulatory StatusFor Research Use Only
ShippingDry ice
StorageStore at -20 °C.
References1. Southall, T. D., & Brand, A. H. (2008). Generation of driver and reporter constructs for the GAL4 expression system in Drosophila. Cold Spring Harbor Protocols, 2008(7), pdb-prot5029.
2. Klueg, K. M., Alvarado, D., Muskavitch, M. A., & Duffy, J. B. (2002). Creation of a GAL4/UAS-coupled inducible gene expression system for use in Drosophila cultured cell lines. genesis, 34(1-2), 119-122.
For Research Use Only | Not For Clinical Use.
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