GV3101(pSoup-P19) electro competent cells (MOFY-0822-FY525)


Cat: MOFY-0822-FY525
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DescriptionP19 protein is derived from tomato bush dwarf virus, which can inhibit the RNA silencing effect of the host on foreign genes, improve the stability of heterologous gene transcripts, and then promote the expression of heterologous proteins. It is widely used in transgenic plants and tobacco leaves, Arabidopsis thaliana Leaf, tomato leaf or protoplast transient expression system. The GV3101 strain is a C58 type background, and the nuclear gene contains a screening tag-rifampicin resistance gene rif, which is suitable for transgenic operations of plants such as Arabidopsis, tobacco, corn, and potatoes.
Gene TypesC58 (rifR) Ti pMP90 (pTiC58DT-DNA) ( gentR) Nopaline (pSoup-p19-tetR)
Size50 µL/vial
QC resultTransformation efficiency of pGs2 plasmid (kanamycin resistance) detection:>105 cfu/μg DNA; Transformation efficiency of pGs2-ZH plasmid (size: 40 kb) detection:>5×103 cfu/μg DNA.
Note1. When the plasmid is added, the volume should not be greater than 1/10 of the competent volume; if the plasmid is impure or there is organic pollution such as ethanol, the transformation efficiency will drop sharply; if the plasmid is doubled, the transformation efficiency will drop by an order of magnitude.
2. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating.
3. When the density of positive clones on the plate is too large, due to insufficient nutrition, the growth of positive clones will slow down and the colonies will become smaller. In order to obtain large colonies, the amount of plasmid should be reduced.
4. The concentration of rifampicin should not be higher than 25 μg/ml. Too high concentration of rifampicin is not conducive to the growth of Agrobacterium and will reduce its growth rate and transformation efficiency. The plate used by our company to calculate the transformation efficiency only contains 50 μg/ml kan. If the plate used contains 20 μg/ml rif, the transformation efficiency will be reduced to 1/2.
5. The purpose of adding rifampicin to the medium is to prevent the growth of miscellaneous bacteria and to screen Agrobacterium; adding streptomycin or gentamicin according to the resistance of the strain used can prevent the loss of Ti plasmid, but streptomycin is not conducive to the growth of Agrobacterium. Because of transgenic operation, streptomycin or gentamicin is generally not considered when culturing Agrobacterium, and the probability of loss of Ti plasmid is extremely low (can be ignored).

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
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For Research Use Only | Not For Clinical Use.
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