HB101 chemically competent cells (MOFY-0822-FY470)
Cat: MOFY-0822-FY470
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Specifications
| Description | Strain HB101 is a hybrid product of E. Coli K12 strain and E. Coli B strain (also the original strain of Stbl3). The recA13 mutation can effectively inhibit the recombination of the terminal repeat region of the long fragment and reduce the probability of erroneous recombination; however, it does not contain the nuclease endA1 mutation, and the nuclease content in the body is high. When extracting the plasmid, be sure to use the protein removal solution in the plasmid extraction kit. |
| Gene Types | F- mcrB mrr hsdS20 (rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1rpsL20 ( (strR) glnV44λ- |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 5×108 cfu/μg DNA |
| Note | 1. Competent cells should be slowly thawed in ice, and the target DNA should be added within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Be gentle when mixing plasmids or ligation products. 3. Transforming high concentrations of plasmids or high-efficiency ligation products can correspondingly reduce the amount of bacteria that are ultimately used for plating. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
For Research Use Only | Not For Clinical Use.
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