Mach1-T1 chemically competent cells (MOFY-0822-FY461)
Cat: MOFY-0822-FY461
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Specifications
| Description | The Mach1-T1 strain is transformed from the wild-type non-K-12 E. Coli W strain. It is one of the fastest growing competitive cells at present. Cloning can be seen on the plate for 8 hours, and the endonuclease (endA) is deleted. The yield and quality of plasmid DNA are improved; the recombinase-deficient type (recA1398) reduces the probability of homologous recombination of the inserted fragment and ensures the stability of the inserted DNA. |
| Gene Types | F- φ80 (lacZ)ΔM15 ΔlacX74 hsdR (rK- mK+) ΔrecA1398 endA1 tonA |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection>5×109 cfu/μg DNA |
| Note | 1. Competent cells should be slowly thawed in ice, and the target DNA should be added within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Be gentle when mixing plasmids or ligation products. 3. Transforming high concentrations of plasmids or high-efficiency ligation products can correspondingly reduce the amount of bacteria that are ultimately used for plating. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
For Research Use Only | Not For Clinical Use.
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