MC1061F- chemically competent cells (MOFY-0822-FY480)
Cat: MOFY-0822-FY480
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Specifications
| Description | MC1061F-strain is derived from E.coli B/r type SB3118 strain, which is also the parent strain of DH10b. The transformation efficiency of MC1061F-transformed competitive cells can be used for routine plasmid construction and phage display experiments in the laboratory, but it lacks the F` factor and cannot be used for filamentous phage (such as M13) infection. |
| Gene Types | araD139 Δ (araA-leu)7697 Δ (lac)X74 galK16 galE15 (GalS) lambda- e14- mcrA0 relA1 rpsL150 (StrR) spoT1 mcrB1 hsdR2 |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 2×109 cfu/μg DNA |
| Note | 1. Competent cells should be slowly thawed in ice. The target DNA should be added within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Transforming high concentrations of plasmids or high-efficiency ligation products can correspondingly reduce the amount of bacteria that will be used for plating. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
For Research Use Only | Not For Clinical Use.
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