Mouse Anti-E. coli nfsA Antibody (CBMOAB-1841YC)
Cat: CBMOAB-1841YC
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Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | E. coli (Escherichia coli ) |
| Clone | MO1841YC |
| Specificity | This antibody binds to Escherichia coli K-12 nfsA. |
| Format | Liquid or Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Introduction | Catalyzes the reduction of nitroaromatic compounds using NADPH. Has a broad electron acceptor specificity. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major oxygen-insensitive nitroreductase in E.coli. |
| Product Overview | Mouse Anti-E. coli nfsA (clone MO1841YC) Antibody (CBMOAB-1841YC) is a mouse antibody against nfsA. It can be used for nfsA detection in Western Blot and Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | Oxygen-insensitive NADPH nitroreductase; EC 1.-.-.-; Modulator of drug activity A; nfsA; mda18 mdaA ybjB; b0851 JW0835 |
| UniProt ID | P17117 |
| Protein Refseq | The length of the protein is 240 amino acids long. The sequence is show below: MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPFILDYLHKQGWATR. |
See other products for " nfsA "
SDS-PAGE
Figure 1 Mouse Anti-E. coli nfsA (clone MO1841YC) Antibody in SDS-PAGE.
SDS-PAGE analysis of CBMOAB-1841YC in reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
ELISA
Figure 2 Mouse Anti-E. coli nfsA (clone MO1841YC) Antibody in ELISA.
ELISA analysis of CBMOAB-1841YC was performed by coating with recombinant E. coli nfsA antigen peptide.
The abscissa represents the antibody dilution fold (*k).
For Research Use Only | Not For Clinical Use.
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