AibGenesis™ Mouse Anti-gyrB Antibody (CBMOAB-1128YC)

Name: AibGenesis™ Mouse Anti-gyrB Antibody (CBMOAB-1128YC)
SKU: CBMOAB-1128YC
Rating: 5 (1 reviews)

Cat: CBMOAB-1128YC

Certificate of Analysis Lookup
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Lot Number

Product List
Specifications
Application Information
Target

Sub Cat	Clonality	Species Reactivity	Application	Clone	Conjugate	Size
CBMOAB-1128YC	Monoclonal	E. coli (Escherichia coli ), Rice (Oryza)	WB, ELISA	MO1128YC		100 µg	Inquiry
Protein Refseq: The length of the protein is 804 amino acids long. The sequence is show below: MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGETEKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMTRRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATLHTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQTVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFITGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALKAANIDI.
CBMOAB-23013FYB	Monoclonal	Rice (Oryza)	WB, ELISA	MO23013FYB		100 µg	Inquiry
Protein Refseq: The length of the protein is 729 amino acids long. The sequence is show below: MGPLLRSSPPPRHLRLLLRRLLSTAAGRPSRLLPLPASSSARLLVRPRVAVAAAAAGAPLRRNGVAVRAFMASTAASEAMQEKRVAGEYTAANVQVLEALDGVRTRPGMYIGSTGSRGLHHLVYEILDNAVDEAQAGYATKVDVILHGDNSVSVTDNGRGIPTDIHPQTKKSCVETVLTLMHAGGKFGGSKSGYTVSGGLHGVGLSVVNALSEALEVTVWRDGKEYRQNYSRGKAITMLTSRTLSDESSSRQGTRIRFWPDKHIFTTTMDFDFNTIAGRIRELAFLNPELTIALTKEEDDLQVQHNEYCYAGGLVEYVKWLNTDKKSLHDPIAFRKEMDGITVDVSLQWCSDSYSDTVLGYANSIRTIDGGTHIDGLKTSLTRTINNFAKKSKTLKDKDISLSGEHVREGMTCIIAVKVPNPEFEGQTKTRLGNPEVRRIVEQSVQENLTEYLELHPDVLDSILSKSLNALKAALAAKRARELVRTKSVLKSSSLPGKLADCASSDPEESEIFIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIERRDEAALYKNEEIQNLIVALGLGVKGEDFNKEALRYHKIVILTDADVDGAHIRTLLLTFFFRYQKALFDEGCIYVGVPPLYKVERGKQAHYCYDDADLKELVNTFPTNASYHIQRFKGLGEMMPAQLWETTMDPERRMLKQLKVEDAAEANVVFSSLMGTRVDVRKQLIQNAASMVNLEHLDI.

Specifications

Host species	Mouse (Mus musculus)
Species Reactivity	E. coli (Escherichia coli ), Rice (Oryza)
Clone	MO1128YC
Specificity	This antibody binds to Escherichia coli K-12 gyrB.
Format	Liquid or Lyophilized
Storage	Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use
Purity	> 90% was determined by SDS-PAGE
Purification	Purified with Protein A or G affinity chromatography

Application Information

Application	WB, ELISA
Application Notes	ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

Introduction	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner to maintain chromosomes in an underwound state (PubMed:186775, PubMed:3031051, PubMed:1323022, PubMed:8248233, PubMed:7811004, PubMed:8621650, PubMed:9657678, PubMed:12051842, PubMed:12051843, PubMed:18642932, PubMed:19060136, PubMed:19965760, PubMed:22457353, PubMed:23294697, PubMed:20356737, PubMed:20675723, PubMed:23352267, PubMed:24386374, PubMed:25202966, PubMed:25849408). This makes better substrates for topoisomerase 4 (ParC and ParE) which is the main enzyme that unlinks newly replicated chromosomes in E.coli (PubMed:9334322). Gyrase catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes (PubMed:22457352). Relaxes negatively supercoiled DNA in an ATP-independent manner (PubMed:337300). E.coli gyrase has higher supercoiling activity than other characterized bacterial gyrases; at comparable concentrations E.coli gyrase introduces more supercoils faster than M.tuberculosis gyrase, while M.tuberculosis gyrase has higher decatenation than supercoiling activity compared to E.coli (PubMed:22457352). E.coli makes 15% more negative supercoils in pBR322 plasmid DNA than S.typhimurium; the S.typhimurium GyrB subunit is toxic in E.coli, while the E.coli copy can be expressed in S.typhimurium even though the 2 subunits have 777/804 residues identical (PubMed:17400739). The enzymatic differences between E.coli gyrase and topoisomerase IV are largely due to the GyrA C-terminal domain (approximately residues 524-841) and specifically the GyrA-box (PubMed:8962066, PubMed:16332690). (From uniprot, under CC BY 4.0)
Product Overview	Mouse Anti-E. coli gyrB Antibody is a mouse antibody against gyrB. It can be used for gyrB detection in Western Blot and Enzyme-Linked Immunosorbent Assay.
Alternative Names	DNA gyrase subunit B; EC 5.99.1.3; gyrB; acrB himB hisU nalC parA pcbA; b3699 JW5625
UniProt ID	P0AES6
Protein Refseq	The length of the protein is 804 amino acids long. The sequence is show below: MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGETEKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMTRRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATLHTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQTVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFITGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALKAANIDI.

For Research Use Only | Not For Clinical Use.

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