Mouse Anti-tgt Antibody (CBMOAB-2721YC)

Name: Mouse Anti-tgt Antibody (CBMOAB-2721YC)
SKU: CBMOAB-2721YC
Rating: 5 (1 reviews)

Cat: CBMOAB-2721YC

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Lot Number

Product List
Specifications
Application Information
Target

Sub Cat	Clonality	Species Reactivity	Application	Clone	Conjugate	Size
CBMOAB-2721YC	Monoclonal	E. coli (Escherichia coli ), Fruit fly (Drosophila melanogaster)	WB, ELISA	MO2721YC		100 µg	Inquiry
Protein Refseq: The length of the protein is 375 amino acids long. The sequence is show below: MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD.
CBMOAB-32954FYA	Monoclonal	Fruit fly (Drosophila melanogaster)	WB, ELISA	MO32954FYA		100 µg	Inquiry
Protein Refseq: The length of the protein is 427 amino acids long. The sequence is show below: MGPSHIPPLTYKVVAECSVSKARAGLMTLRHSEVNTPVFMPVGTQGTLKGIVPDQLIELNCQILLGNTYHLGLRPGIETLKKAGGLHKFMGWPRAILTDSGGFQMVSLLQLAEIDEHGVNFRSPFDNSQCMLTPEHSIEIQNAIGGDIMMQLDDVVKTTTTGPRVEEAMERTIRWVDRCIEAHARDDDQSLFPIVQGGLDVPLRQRCVSALMERQVRGFAVGGLSGGESKHDFWRMVDVCTGYLPKDKPRYLMGVGFAADLVVCVALGIDMFDCVFPTRTARFGCALVDSGQLNLKQPKYKLDMEPIDKDCDCSTCRRYTRSYLHHIATNESVSSSLLSIHNVAYQLRLMRSMREAIQRDEFPQFVADFMARHFKAEPVPAWIREALSAVNIQLPADPERIDEQDQKPKTEKRRETEDVAEEQVASS.

Specifications

Host species	Mouse (Mus musculus)
Species Reactivity	E. coli (Escherichia coli ), Fruit fly (Drosophila melanogaster)
Clone	MO2721YC
Specificity	This antibody binds to Escherichia coli K-12 tgt.
Format	Liquid or Lyophilized
Storage	Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use
Purity	> 90% was determined by SDS-PAGE
Purification	Purified with Protein A or G affinity chromatography

Application Information

Application	WB, ELISA
Application Notes	ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

Introduction	Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Product Overview	Mouse Anti-E. coli tgt Antibody is a mouse antibody against tgt. It can be used for tgt detection in Western Blot and Enzyme-Linked Immunosorbent Assay.
Alternative Names	Queuine tRNA-ribosyltransferase; EC 2.4.2.29; Guanine insertion enzyme; tRNA-guanine transglycosylase; tgt; b0406 JW0396
UniProt ID	P0A847
Protein Refseq	The length of the protein is 375 amino acids long. The sequence is show below: MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD.

For Research Use Only | Not For Clinical Use.

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