p414-TEF1p-Cas9-CYC1t yeast edited Cas9 expression plasmid


Cat: MOFY-0123-FY12
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  • Specifications
  • Plasmid Information

Specifications

Species SourceYeast
Size5 µg
CompositionPromoter: RPR1 promoter;
Prokaryotic resistance: Ampicillin Amp;
Screening marker: LEU2;
Cloned strain: Escherichia coli DH5α;
Culture conditions: 37°C.
BufferRefer to COA

Plasmid Information

Product OverviewpRPR1_gRNA_handle_RPR1t is a gene editing gRNA expression plasmid for yeast cells.
Regulatory StatusFor Research Use Only
ShippingDry ice
StorageStore at -20 °C.
References1. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013 Feb 15;339(6121):819-23.
2. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini LA, Bao G, Zhang F. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol. 2013 Sep;31(9):827-32.
3. Adli M. The CRISPR tool kit for genome editing and beyond. Nat Commun. 2018 May 15;9(1):1911.
For Research Use Only | Not For Clinical Use.
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