pETcoco-1 E. coli expression plasmid

Cat: MOFY-0123-FY48
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Species SourceE. coli
Size3 µg
CompositionProkaryotic resistance: Chloramphenicol Chl;
Cloned strain: Escherichia coli DH5α.
BufferRefer to COA

Plasmid Information

Product OverviewThe pETcoco expression vector is designed to allow "on-command" vector copy number amplification from single copy (1-2 plasmid copies per cell) to medium copy (20-50 per cell). The vector is kept in a single-copy state by utilizing the oriS and repE elements of the F appendage and the parABC partition determinant. Media replication status was obtained by using the RK-2-derived trfA replicon acting on oriV. Switching from the single- to mid-copy state is achieved by inducing the expression of the trfA gene with arabinose. The dual-replicon nature of these vectors promotes enhanced stability of recombinant plasmids and very low basal expression levels in the single-copy state, while allowing robust target protein expression typical of pET vectors. Unique restriction sites are shown on the circle diagram (below). The sequence is numbered relative to the T7 transcription start site +1.
Regulatory StatusFor Research Use Only
ShippingDry ice
StorageStore at -20 °C.
References1. Wild, J., & Szybalski, W. (2004). Copy-control tightly regulated expression vectors based on pBAC/oriV. Recombinant Gene Expression: Reviews and Protocols, 155-167.
2. Saïda, F. (2007). Overview on the expression of toxic gene products in Escherichia coli. Current Protocols in Protein Science, 50(1), 5-19.
For Research Use Only | Not For Clinical Use.
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