pGFPuv E. coli fluorescent plasmid

Cat: MOFY-0123-FY45
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Species SourceE. coli
Size3 µg
CompositionProkaryotic resistance: Ampicillin Amp;
Screening markers: green fluorescent protein GFPuv;
Cloned strain: Escherichia coli DH5α;
Culture conditions: 37°C.
BufferRefer to COA

Plasmid Information

Product OverviewpGFPuv is an Escherichia coli green fluorescent expression plasmid. pGFPuv carries the "cycle 3" variant of GFP described by Crameri et al. (1). The gene was cloned between two MCSs of pUC19 derivative pPD16.43 (2). The GFPuv gene can be easily excised from pGFPuv. Alternatively, the GFPuv coding sequence can be amplified by PCR. The GFPuv gene was inserted in-frame with the lacZ initiation codon from pUC19 to allow expression of the β-galactosidase-GFPuv fusion protein from the lac promoter in E. coli. Note, however, that if you excise the GFPuv coding sequence using the restriction sites in the 5' MCS, the resulting fragment will encode the native (i.e. non-fused) GFPuv protein. The pUC backbone of pGFPuv provides a high copy number origin of replication and an ampicillin resistance gene for propagation in E. coli.
Regulatory StatusFor Research Use Only
ShippingDry ice
StorageStore at -20 °C.
References1. Ma, L., Zhang, G., & Doyle, M. P. (2011). Green fluorescent protein labeling of Listeria, Salmonella, and Escherichia coli O157: H7 for safety-related studies. PloS one, 6(4), e18083.
2. Salmon-Divon, M., Nitzan, Y., & Malik, Z. (2004). Mechanistic aspects of Escherichia coli photodynamic inactivation by cationic tetra-meso (N-methylpyridyl) porphine. Photochemical & Photobiological Sciences, 3(5), 423-429.
For Research Use Only | Not For Clinical Use.

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