pGilda yeast two-hybrid plasmid

Cat: MOFY-0123-FY20

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Specifications
Plasmid Information

Specifications

Species Source	Yeast
Size	2 µg
Composition	Prokaryotic resistance: Ampicillin Amp; Screening marker: HIS3; Cloned strain: Escherichia coli DH5α; Culture conditions: 37°C.
Buffer	Refer to COA

Plasmid Information

Product Overview	pGilda is a cloning vector used to generate fusions of a bait protein with the 202-residue LexA protein, which acts as a DNA-binding domain in the MATCHMAKER LexA Two-Hybrid System . The hybrid protein is expressed in yeast cells from the tightly regulated GAL1 promoter (PGAL1); transcription is repressed in the presence of glucose and induced in the the absence of glucose and presence of galactose. pGilda carries the HIS3 gene for selection in His- auxotrophic yeast strains and is compatible with MATCHMAKER LexA Libraries. This vector replicates autonomously in both E. coli (using the Col E1 ori) and in S. cerevisiae (using the CEN/ARS ori). Amp is used for selection in E. coli.
Regulatory Status	For Research Use Only
Shipping	Dry ice
Storage	Store at -20 °C.
References	1. Araya, R., Takahashi, R., & Nomura, Y. (2002). Yeast two-hybrid screening using constitutive-active caspase-7 as bait in the identification of PA28γ as an effector caspase substrate. Cell Death & Differentiation, 9(3), 322-328. 2. Lee, Y. Y., Chang, C. F., Kuo, C. L., Chen, M. C., Yu, C. H., Lin, P. I., & Wu, W. F. (2003). Subunit oligomerization and substrate recognition of the Escherichia coli ClpYQ (HslUV) protease implicated by in vivo protein-protein interactions in the yeast two-hybrid system. Journal of Bacteriology, 185(8), 2393-2401.

For Research Use Only | Not For Clinical Use.

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